IR@PKUHSC  > 北京大学第一临床医学院  > 临床药理研究所
学科主题临床医学
A novel gene, optrA, that confers transferable resistance to oxazolidinones and phenicols and its presence in Enterococcus faecalis and Enterococcus faecium of human and animal origin
Wang, Yang1; Lv, Yuan2; Cai, Jiachang3; Schwarz, Stefan4; Cui, Lanqing2; Hu, Zhidong5; Zhang, Rong3; Li, Jun1; Zhao, Qin1; He, Tao1; Wang, Dacheng6; Wang, Zheng1; Shen, Yingbo1; Li, Yun2; Fessler, Andrea T.4; Wu, Congming1; Yu, Hao6; Deng, Xuming6; Xia, Xi7; Shen, Jianzhong1
关键词linezolid tedizolid florfenicol plasmids interspecies transfer enterococci
刊名JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
2015-08-01
DOI10.1093/jac/dkv116
70期:8页:2182-2190
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Infectious Diseases ; Microbiology ; Pharmacology & Pharmacy
资助者National Basic Research Program of China ; National Natural Science Foundation of China ; German Federal Ministry of Education and Research (BMBF) through the German Aerospace Center (DLR) ; National Basic Research Program of China ; National Natural Science Foundation of China ; German Federal Ministry of Education and Research (BMBF) through the German Aerospace Center (DLR)
研究领域[WOS]Infectious Diseases ; Microbiology ; Pharmacology & Pharmacy
关键词[WOS]STAPHYLOCOCCUS-AUREUS ; METHICILLIN-RESISTANT ; LINEZOLID-RESISTANT ; VANCOMYCIN ; INFECTIONS ; ANTIBIOTICS ; CFR ; PLEUROMUTILINS ; IDENTIFICATION ; LINCOSAMIDES
英文摘要

Objectives: The oxazolidinone-resistant Enterococcus faecalis E349 from a human patient tested negative for the cfr gene and 23S rRNA mutations. Here we report the identification of a novel oxazolidinone resistance gene, optrA, and a first investigation of the extent to which this gene was present in E. faecalis and Enterococcus faecium from humans and food-producing animals.

Methods: The resistance gene optrA was identified by whole-plasmid sequencing and subsequent cloning and expression in a susceptible Enterococcus host. Transformation and conjugation assays served to investigate the transferability of optrA. All optrA-positive E. faecalis and E. faecium isolates of human and animal origin were analysed for their MICs and their genotype, as well as the location of optrA.

Results: The novel plasmid-borne ABC transporter gene optrA from E. faecalis E349 conferred combined resistance or elevated MICs (when no clinical breakpoints were available) to oxazolidinones (linezolid and tedizolid) and phenicols (chloramphenicol and florfenicol). The corresponding conjugative plasmid pE349, on which optrA was located, had a size of 36331 bp and also carried the phenicol exporter gene fexA. The optrA gene was functionally expressed in E. faecalis, E. faecium and Staphylococcus aureus. It was detected more frequently in E. faecalis and E. faecium from food-producing animals (20.3% and 5.7%, respectively) than from humans (4.2% and 0.6%, respectively).

Conclusions: Enterococci with elevated MICs of linezolid and tedizolid should be tested not only for 23S rRNA mutations and the gene cfr, but also for the novel resistance gene optrA.

语种英语
所属项目编号2013CB127200 ; 31370046 ; 01KI1301D
资助者National Basic Research Program of China ; National Natural Science Foundation of China ; German Federal Ministry of Education and Research (BMBF) through the German Aerospace Center (DLR) ; National Basic Research Program of China ; National Natural Science Foundation of China ; German Federal Ministry of Education and Research (BMBF) through the German Aerospace Center (DLR)
WOS记录号WOS:000359723600002
引用统计
被引频次:57[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64547
专题北京大学第一临床医学院_临床药理研究所
作者单位1.Peking Univ, Hosp 1, Inst Clin Pharmacol, Beijing 100871, Peoples R China
2.FLI, Inst Farm Anim Genet, Neustadt, Germany
3.Tianjin Med Univ Gen Hosp, Tianjin, Peoples R China
4.China Agr Univ, Coll Vet Med, Dept Vet Pharmacol, Beijing 100094, Peoples R China
5.Zhejiang Univ, Affiliated Hosp 2, Hangzhou 310003, Zhejiang, Peoples R China
6.Jilin Univ, Coll Vet Med, Inst Zoonosis, Changchun 130023, Peoples R China
7.China Agr Univ, Beijing Key Lab Detect Technol Anim Food Safety, Beijing 100094, Peoples R China
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GB/T 7714
Wang, Yang,Lv, Yuan,Cai, Jiachang,et al. A novel gene, optrA, that confers transferable resistance to oxazolidinones and phenicols and its presence in Enterococcus faecalis and Enterococcus faecium of human and animal origin[J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY,2015,70(8):2182-2190.
APA Wang, Yang.,Lv, Yuan.,Cai, Jiachang.,Schwarz, Stefan.,Cui, Lanqing.,...&Shen, Jianzhong.(2015).A novel gene, optrA, that confers transferable resistance to oxazolidinones and phenicols and its presence in Enterococcus faecalis and Enterococcus faecium of human and animal origin.JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY,70(8),2182-2190.
MLA Wang, Yang,et al."A novel gene, optrA, that confers transferable resistance to oxazolidinones and phenicols and its presence in Enterococcus faecalis and Enterococcus faecium of human and animal origin".JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY 70.8(2015):2182-2190.
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