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学科主题: 基础医学
题名:
Cloning of renal cell carcinoma relation gene: Construction of a cDNA subtractive library of human renal cell carcinoma and its significance.
作者: Zhang, Q; Mao, ZB; Zhang, ZW; Xin, DQ; Guo, YL
关键词: kidney neoplasms carcinoma ; suppression subtractive hybridization ; library
刊名: PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
发表日期: 2000-06-01
卷: 27, 期:3, 页:301-304
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: C-MYC ; SUPPRESSION ; HYBRIDIZATION
英文摘要:

To construct a cDNA subtractive library of human renal cell carcinoma (RCC) with technique called suppression subtractive hybridization. The library only contains the differently expressing cDNAs between RCC and normal kidney. Poly (A)(+) RNA were isolated from cell lines of RCC and normal kidney respectively. Moreover, single-strand cDNAs and double-strand cDNAs were synthesized in turn. After enzyme restriction, cDNAs between 400 similar to 600 bp were obtained. RCC cDNAs then were divided into two groups and ligated to. the specific adaptor 1 and adaptor 2 respectively. After RCC cDNAs hybridized with normal kidney cDNA twice and underwent two times of nested PCR, then with arms of T/A plasmid vectors to set up the subtractive library. Amplification of the library was carried out with the E. coli strain Top 10F′. Human RCC subtrctive library with high subtractive efficiency was set up sucessfully. The amplified library contains 6 500 positive clones. Random analysis of 350 clones with enzyme restriction shows that all plasmids in the clones contain 400 similar to 600 bp inserts. The constructed cDNA subtractive library of human RCC is a highly efficient one and lays solid foundation for screening and cloning new and specific oncogenes or tumor suppressor genes of RCC.

语种: 中文
WOS记录号: WOS:000087859200020
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64567
Appears in Collections:基础医学院_生理学与病理生理学系_期刊论文

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作者单位: 1.Beijing Med Univ, Dept Physiol, Beijing 100083, Peoples R China
2.Beijing Med Univ, Teaching Hosp 1, Dept Urol, Beijing 100034, Peoples R China

Recommended Citation:
Zhang, Q,Mao, ZB,Zhang, ZW,et al. Cloning of renal cell carcinoma relation gene: Construction of a cDNA subtractive library of human renal cell carcinoma and its significance.[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2000,27(3):301-304.
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