学科主题基础医学
Cloning of renal cell carcinoma relation gene: Construction of a cDNA subtractive library of human renal cell carcinoma and its significance.
Zhang, Q; Mao, ZB; Zhang, ZW; Xin, DQ; Guo, YL
关键词kidney neoplasms carcinoma suppression subtractive hybridization library
刊名PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
2000-06-01
27期:3页:301-304
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]C-MYC ; SUPPRESSION ; HYBRIDIZATION
英文摘要

To construct a cDNA subtractive library of human renal cell carcinoma (RCC) with technique called suppression subtractive hybridization. The library only contains the differently expressing cDNAs between RCC and normal kidney. Poly (A)(+) RNA were isolated from cell lines of RCC and normal kidney respectively. Moreover, single-strand cDNAs and double-strand cDNAs were synthesized in turn. After enzyme restriction, cDNAs between 400 similar to 600 bp were obtained. RCC cDNAs then were divided into two groups and ligated to. the specific adaptor 1 and adaptor 2 respectively. After RCC cDNAs hybridized with normal kidney cDNA twice and underwent two times of nested PCR, then with arms of T/A plasmid vectors to set up the subtractive library. Amplification of the library was carried out with the E. coli strain Top 10F′. Human RCC subtrctive library with high subtractive efficiency was set up sucessfully. The amplified library contains 6 500 positive clones. Random analysis of 350 clones with enzyme restriction shows that all plasmids in the clones contain 400 similar to 600 bp inserts. The constructed cDNA subtractive library of human RCC is a highly efficient one and lays solid foundation for screening and cloning new and specific oncogenes or tumor suppressor genes of RCC.

语种中文
WOS记录号WOS:000087859200020
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64567
专题北京大学基础医学院_生理学与病理生理学系
作者单位1.Beijing Med Univ, Dept Physiol, Beijing 100083, Peoples R China
2.Beijing Med Univ, Teaching Hosp 1, Dept Urol, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Q,Mao, ZB,Zhang, ZW,et al. Cloning of renal cell carcinoma relation gene: Construction of a cDNA subtractive library of human renal cell carcinoma and its significance.[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2000,27(3):301-304.
APA Zhang, Q,Mao, ZB,Zhang, ZW,Xin, DQ,&Guo, YL.(2000).Cloning of renal cell carcinoma relation gene: Construction of a cDNA subtractive library of human renal cell carcinoma and its significance..PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,27(3),301-304.
MLA Zhang, Q,et al."Cloning of renal cell carcinoma relation gene: Construction of a cDNA subtractive library of human renal cell carcinoma and its significance.".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 27.3(2000):301-304.
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