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学科主题: 基础医学
题名:
Cross-sectional shape of rat mesenteric arterioles at branching studied by confocal laser microscopy
作者: Nakano, A; Minamiyama, M; Niimi, H
关键词: arteriole ; branching ; confocal laser scanning microscopy ; cross-section ; 3D reconstruction ; mesentery ; microcirculation
刊名: JSME INTERNATIONAL JOURNAL SERIES C-MECHANICAL SYSTEMS MACHINE ELEMENTS AND MANUFACTURING
发表日期: 2006-03-01
卷: 49, 期:1, 页:240-246
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Engineering, Manufacturing ; Engineering, Mechanical
研究领域[WOS]: Engineering
关键词[WOS]: DIAMETER VARIABILITY ; RABBIT MESENTERY ; FLOW RESISTANCE ; CAPILLARY-FLOW ; HETEROGENEITY ; MUSCLE ; MICROCIRCULATION ; ARCHITECTURE ; PERICYTES ; VESSELS
英文摘要:

This study was aimed to investigate the cross-sectional shape of mesenteric arterioles at branching, using confocal laser microscopy. Wistar rats (8 weeks, male) were anesthetized with thiobutabarbital sodium. Blood flow and microvascular network in the mesentery were observed using video microscopy. The rat intestine with mesentery was extracted and the intestinal vasculature was perfused with Krebs-Ringer and then fixed with paraformaldehyde under a static pressure of 100mmHg. A section of mesentery was isolated from the intestine, and spread up to the in vivo geometry based on the intravital microscopic observation. The mesentery section was stained with tetramethyl rhodamine isothiocyanate (TRITC)-phalloidin. The samples were observed under a confocal laser microscope. The cross-sectional image was re-sliced to measure the cross-sectional area and major/minor axes of the best fitting ellipse. The aspect ratio was defined in terms of the minor/major diameter ratio. The extended focus image of mesenteric arterioles showed that the cross-sectional shape was not circular but elliptic-like. The cross-sectional area of the parent vessel decreased from proximal to distal positions. The mean aspect ratio of the parent vessel was approximately 0.5, while that of the branching vessel was approximately 0.8. The flattened shape and variation of the cross-sectional area of arterioles requires some correction of in vivo data of the two-dimensional mesenteric microvasculature obtained using intravital microscopy.

语种: 英语
WOS记录号: WOS:000237168800033
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64572
Appears in Collections:基础医学院_北大医学部天士力微循环研究中心_期刊论文

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作者单位: 1.Natl Cardiovasc Ctr, Res Inst, Dept Vasc Physiol, Suita, Osaka 5658565, Japan
2.Hiroshima Int Univ, Dept Clin Engn, Higashihiroshima 7240695, Japan
3.Peking Univ, Tasly Microcirculat Res Ctr, Ctr Hlth Sci, Beijing 100083, Peoples R China

Recommended Citation:
Nakano, A,Minamiyama, M,Niimi, H. Cross-sectional shape of rat mesenteric arterioles at branching studied by confocal laser microscopy[J]. JSME INTERNATIONAL JOURNAL SERIES C-MECHANICAL SYSTEMS MACHINE ELEMENTS AND MANUFACTURING,2006,49(1):240-246.
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