学科主题基础医学
Cross-sectional shape of rat mesenteric arterioles at branching studied by confocal laser microscopy
Nakano, A; Minamiyama, M; Niimi, H
关键词arteriole branching confocal laser scanning microscopy cross-section 3D reconstruction mesentery microcirculation
刊名JSME INTERNATIONAL JOURNAL SERIES C-MECHANICAL SYSTEMS MACHINE ELEMENTS AND MANUFACTURING
2006-03-01
49期:1页:240-246
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Manufacturing ; Engineering, Mechanical
研究领域[WOS]Engineering
关键词[WOS]DIAMETER VARIABILITY ; RABBIT MESENTERY ; FLOW RESISTANCE ; CAPILLARY-FLOW ; HETEROGENEITY ; MUSCLE ; MICROCIRCULATION ; ARCHITECTURE ; PERICYTES ; VESSELS
英文摘要

This study was aimed to investigate the cross-sectional shape of mesenteric arterioles at branching, using confocal laser microscopy. Wistar rats (8 weeks, male) were anesthetized with thiobutabarbital sodium. Blood flow and microvascular network in the mesentery were observed using video microscopy. The rat intestine with mesentery was extracted and the intestinal vasculature was perfused with Krebs-Ringer and then fixed with paraformaldehyde under a static pressure of 100mmHg. A section of mesentery was isolated from the intestine, and spread up to the in vivo geometry based on the intravital microscopic observation. The mesentery section was stained with tetramethyl rhodamine isothiocyanate (TRITC)-phalloidin. The samples were observed under a confocal laser microscope. The cross-sectional image was re-sliced to measure the cross-sectional area and major/minor axes of the best fitting ellipse. The aspect ratio was defined in terms of the minor/major diameter ratio. The extended focus image of mesenteric arterioles showed that the cross-sectional shape was not circular but elliptic-like. The cross-sectional area of the parent vessel decreased from proximal to distal positions. The mean aspect ratio of the parent vessel was approximately 0.5, while that of the branching vessel was approximately 0.8. The flattened shape and variation of the cross-sectional area of arterioles requires some correction of in vivo data of the two-dimensional mesenteric microvasculature obtained using intravital microscopy.

语种英语
WOS记录号WOS:000237168800033
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64572
专题北京大学基础医学院_北大医学部天士力微循环研究中心
作者单位1.Natl Cardiovasc Ctr, Res Inst, Dept Vasc Physiol, Suita, Osaka 5658565, Japan
2.Hiroshima Int Univ, Dept Clin Engn, Higashihiroshima 7240695, Japan
3.Peking Univ, Tasly Microcirculat Res Ctr, Ctr Hlth Sci, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Nakano, A,Minamiyama, M,Niimi, H. Cross-sectional shape of rat mesenteric arterioles at branching studied by confocal laser microscopy[J]. JSME INTERNATIONAL JOURNAL SERIES C-MECHANICAL SYSTEMS MACHINE ELEMENTS AND MANUFACTURING,2006,49(1):240-246.
APA Nakano, A,Minamiyama, M,&Niimi, H.(2006).Cross-sectional shape of rat mesenteric arterioles at branching studied by confocal laser microscopy.JSME INTERNATIONAL JOURNAL SERIES C-MECHANICAL SYSTEMS MACHINE ELEMENTS AND MANUFACTURING,49(1),240-246.
MLA Nakano, A,et al."Cross-sectional shape of rat mesenteric arterioles at branching studied by confocal laser microscopy".JSME INTERNATIONAL JOURNAL SERIES C-MECHANICAL SYSTEMS MACHINE ELEMENTS AND MANUFACTURING 49.1(2006):240-246.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
49_240.pdf(1438KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Nakano, A]的文章
[Minamiyama, M]的文章
[Niimi, H]的文章
百度学术
百度学术中相似的文章
[Nakano, A]的文章
[Minamiyama, M]的文章
[Niimi, H]的文章
必应学术
必应学术中相似的文章
[Nakano, A]的文章
[Minamiyama, M]的文章
[Niimi, H]的文章
相关权益政策
暂无数据
收藏/分享
文件名: 49_240.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。