IR@PKUHSC  > 北京大学第一临床医学院  > 普通外科
学科主题临床医学
MicroRNA-19a targets tissue factor to inhibit colon cancer cells migration and invasion
Yu, Ge; Li, Hui; Wang, Xin; Wu, Tao; Zhu, Jing; Huang, Shanjun; Wan, Yuanlian; Tang, Jianqiang
关键词Colon cancer MicroRNA Tissue factor Metastasis
刊名MOLECULAR AND CELLULAR BIOCHEMISTRY
2013-08-01
DOI10.1007/s11010-013-1679-6
380期:1-2页:239-247
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]COLORECTAL-CANCER ; FACTOR EXPRESSION ; HEPATOCELLULAR-CARCINOMA ; HEPATIC METASTASIS ; ANGIOGENESIS ; PROLIFERATION ; PATHOGENESIS ; PROGRESSION ; PHENOTYPE ; PROGNOSIS
英文摘要

The over-expression of tissue factor (TF) and its roles in colon cancer progression have attracted much attention. However, the mechanisms regulating TF expression have not yet been shown in detail. In this study, we over-expressed miR-19a, miR20a and miR-106b in colon cancer cells, and evaluated their impact on TF expression and cellular function. We provide evidence demonstrating that miR-19a inhibited TF expression in vitro. Luciferase reporter assay confirmed that TF was a direct target of miR-19a because the miR-19a mediated repression of luciferase activity was abolished by mutation of the putative binding site. Moreover, miR-19a suppressed colon cancer cell migration and invasion. This effect was due to the indirect down-regulation of matrix metalloproteinase 9. Finally, we investigated the relevance of TF and miR-19a expression in a total of 48 paired colon cancer samples and revealed that miR-19a was inversely correlated with TF expression in stages I and II cases. Therefore, our results suggested that miR-19a was capable of suppressing TF expression in vitro and inhibiting cell migration and invasion. Although it was not the unique mechanism responsible for the expression of TF in vivo, miR-19a was inversely correlated with TF expression in early stage colon cancer patients.

语种英语
WOS记录号WOS:000321131100025
引用统计
被引频次:31[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64594
专题北京大学第一临床医学院_普通外科
作者单位Peking Univ, Dept Gen Surg, Hosp 1, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Yu, Ge,Li, Hui,Wang, Xin,et al. MicroRNA-19a targets tissue factor to inhibit colon cancer cells migration and invasion[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY,2013,380(1-2):239-247.
APA Yu, Ge.,Li, Hui.,Wang, Xin.,Wu, Tao.,Zhu, Jing.,...&Tang, Jianqiang.(2013).MicroRNA-19a targets tissue factor to inhibit colon cancer cells migration and invasion.MOLECULAR AND CELLULAR BIOCHEMISTRY,380(1-2),239-247.
MLA Yu, Ge,et al."MicroRNA-19a targets tissue factor to inhibit colon cancer cells migration and invasion".MOLECULAR AND CELLULAR BIOCHEMISTRY 380.1-2(2013):239-247.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yu, Ge]的文章
[Li, Hui]的文章
[Wang, Xin]的文章
百度学术
百度学术中相似的文章
[Yu, Ge]的文章
[Li, Hui]的文章
[Wang, Xin]的文章
必应学术
必应学术中相似的文章
[Yu, Ge]的文章
[Li, Hui]的文章
[Wang, Xin]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。