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学科主题: 临床医学
题名:
MicroRNA-19a targets tissue factor to inhibit colon cancer cells migration and invasion
作者: Yu, Ge; Li, Hui; Wang, Xin; Wu, Tao; Zhu, Jing; Huang, Shanjun; Wan, Yuanlian; Tang, Jianqiang
关键词: Colon cancer ; MicroRNA ; Tissue factor ; Metastasis
刊名: MOLECULAR AND CELLULAR BIOCHEMISTRY
发表日期: 2013-08-01
DOI: 10.1007/s11010-013-1679-6
卷: 380, 期:1-2, 页:239-247
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: COLORECTAL-CANCER ; FACTOR EXPRESSION ; HEPATOCELLULAR-CARCINOMA ; HEPATIC METASTASIS ; ANGIOGENESIS ; PROLIFERATION ; PATHOGENESIS ; PROGRESSION ; PHENOTYPE ; PROGNOSIS
英文摘要:

The over-expression of tissue factor (TF) and its roles in colon cancer progression have attracted much attention. However, the mechanisms regulating TF expression have not yet been shown in detail. In this study, we over-expressed miR-19a, miR20a and miR-106b in colon cancer cells, and evaluated their impact on TF expression and cellular function. We provide evidence demonstrating that miR-19a inhibited TF expression in vitro. Luciferase reporter assay confirmed that TF was a direct target of miR-19a because the miR-19a mediated repression of luciferase activity was abolished by mutation of the putative binding site. Moreover, miR-19a suppressed colon cancer cell migration and invasion. This effect was due to the indirect down-regulation of matrix metalloproteinase 9. Finally, we investigated the relevance of TF and miR-19a expression in a total of 48 paired colon cancer samples and revealed that miR-19a was inversely correlated with TF expression in stages I and II cases. Therefore, our results suggested that miR-19a was capable of suppressing TF expression in vitro and inhibiting cell migration and invasion. Although it was not the unique mechanism responsible for the expression of TF in vivo, miR-19a was inversely correlated with TF expression in early stage colon cancer patients.

语种: 英语
所属项目编号: 30801092 ; 81272710 ; 30872469
项目资助者: National Natural and Science Foundation of China ; National Natural and Science of Youth Foundation of China
WOS记录号: WOS:000321131100025
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64594
Appears in Collections:北京大学第一临床医学院_普通外科_期刊论文

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作者单位: Peking Univ, Dept Gen Surg, Hosp 1, Beijing 100034, Peoples R China

Recommended Citation:
Yu, Ge,Li, Hui,Wang, Xin,et al. MicroRNA-19a targets tissue factor to inhibit colon cancer cells migration and invasion[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY,2013,380(1-2):239-247.
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