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学科主题: 临床医学
题名:
Ghrelin promotes differentiation of human embryonic stem cells into cardiomyocytes
作者: Yang, Jin2,3; Liu, Guo-qiang2,3; Wei, Rui2,3; Hou, Wen-fang2,3; Gao, Mei-juan2,3; Zhu, Ming-xia1,3; Wang, Hai-ning2,3; Chen, Gui-an1,3; Hong, Tian-pei2,3
关键词: cardiomyocytes ; differentiation ; ghrelin ; embryonic stem cells
刊名: ACTA PHARMACOLOGICA SINICA
发表日期: 2011-10-01
DOI: 10.1038/aps.2011.79
卷: 32, 期:10, 页:1239-1245
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: HORMONE SECRETAGOGUE RECEPTOR ; HEART-FAILURE ; PEPTIDE ; ADIPOGENESIS ; STOMACH ; LINES ; RATS ; 1A
英文摘要:

Aim: Ghrelin is involved in regulating the differentiation of mesoderm-derived precursor cells. The aim of this study was to investigate whether ghrelin modulated the differentiation of human embryonic stem (hES) cells into cardiomyocytes and, if so, whether the effect was mediated by growth hormone secretagogue receptor 1 alpha (GHS-R1 alpha).

Methods: Cardiomyocyte differentiation from hES cells was performed according to an embryoid body (EB)-based protocol. The cumulative percentage of beating EBs was calculated. The expression of cardiac-specific markers including cardiac troponin I (cTnI) and a-myosin heavy chain (alpha-MHC) was detected using RT-PCR, real-time PCR and Western blot. The dispersed beating EBs were examined using immunofluorescent staining.

Results: The percentage of beating EBs and the expression of cTnI were significantly increased after ghrelin (0.1 and 1 nmol/L) added into the differentiation medium. From 6 to 18 d of differentiation, the increased expression of cTnI and alpha-MHC by ghrelin (1 nmol/L) was time-dependent, and in line with the alteration of the percentages of beating EBs. Furthermore, the dispersed beating EBs were double-positively immunostained with antibodies against cTnI and alpha-actinin. However, blockage of GHS-R1 alpha with its specific antagonist D-[lys(3)]-GHRP-6 (1 mu mol/L) did not alter the effects of ghrelin on cardiomyocyte differentiation.

Conclusion: Our data show that ghrelin enhances the generation of cardiomyocytes from hES cells, which is not mediated via GHS-R1 alpha.

语种: 英语
所属项目编号: 2006AA02A112 ; 30771032 ; 30700879 ; 81070701 ; 81000315 ; 20100001110083
项目资助者: Chinese National 863 Program ; National Natural Sciences Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education
WOS记录号: WOS:000295921300008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64603
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Reprod Med Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Dept Endocrinol & Metab, Beijing 100191, Peoples R China
3.Peking Univ, Hosp 3, Clin Stem Cell Res Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Yang, Jin,Liu, Guo-qiang,Wei, Rui,et al. Ghrelin promotes differentiation of human embryonic stem cells into cardiomyocytes[J]. ACTA PHARMACOLOGICA SINICA,2011,32(10):1239-1245.
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