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Ghrelin promotes differentiation of human embryonic stem cells into cardiomyocytes
Yang, Jin2,3; Liu, Guo-qiang2,3; Wei, Rui2,3; Hou, Wen-fang2,3; Gao, Mei-juan2,3; Zhu, Ming-xia1,3; Wang, Hai-ning2,3; Chen, Gui-an1,3; Hong, Tian-pei2,3
关键词cardiomyocytes differentiation ghrelin embryonic stem cells
刊名ACTA PHARMACOLOGICA SINICA
2011-10-01
DOI10.1038/aps.2011.79
32期:10页:1239-1245
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
资助者Chinese National 863 Program ; National Natural Sciences Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education ; Chinese National 863 Program ; National Natural Sciences Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]HORMONE SECRETAGOGUE RECEPTOR ; HEART-FAILURE ; PEPTIDE ; ADIPOGENESIS ; STOMACH ; LINES ; RATS ; 1A
英文摘要

Aim: Ghrelin is involved in regulating the differentiation of mesoderm-derived precursor cells. The aim of this study was to investigate whether ghrelin modulated the differentiation of human embryonic stem (hES) cells into cardiomyocytes and, if so, whether the effect was mediated by growth hormone secretagogue receptor 1 alpha (GHS-R1 alpha).

Methods: Cardiomyocyte differentiation from hES cells was performed according to an embryoid body (EB)-based protocol. The cumulative percentage of beating EBs was calculated. The expression of cardiac-specific markers including cardiac troponin I (cTnI) and a-myosin heavy chain (alpha-MHC) was detected using RT-PCR, real-time PCR and Western blot. The dispersed beating EBs were examined using immunofluorescent staining.

Results: The percentage of beating EBs and the expression of cTnI were significantly increased after ghrelin (0.1 and 1 nmol/L) added into the differentiation medium. From 6 to 18 d of differentiation, the increased expression of cTnI and alpha-MHC by ghrelin (1 nmol/L) was time-dependent, and in line with the alteration of the percentages of beating EBs. Furthermore, the dispersed beating EBs were double-positively immunostained with antibodies against cTnI and alpha-actinin. However, blockage of GHS-R1 alpha with its specific antagonist D-[lys(3)]-GHRP-6 (1 mu mol/L) did not alter the effects of ghrelin on cardiomyocyte differentiation.

Conclusion: Our data show that ghrelin enhances the generation of cardiomyocytes from hES cells, which is not mediated via GHS-R1 alpha.

语种英语
所属项目编号2006AA02A112 ; 30771032 ; 30700879 ; 81070701 ; 81000315 ; 20100001110083
资助者Chinese National 863 Program ; National Natural Sciences Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education ; Chinese National 863 Program ; National Natural Sciences Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education
WOS记录号WOS:000295921300008
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64603
专题北京大学第三临床医学院_生殖医学中心
作者单位1.Peking Univ, Hosp 3, Reprod Med Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Dept Endocrinol & Metab, Beijing 100191, Peoples R China
3.Peking Univ, Hosp 3, Clin Stem Cell Res Ctr, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Yang, Jin,Liu, Guo-qiang,Wei, Rui,et al. Ghrelin promotes differentiation of human embryonic stem cells into cardiomyocytes[J]. ACTA PHARMACOLOGICA SINICA,2011,32(10):1239-1245.
APA Yang, Jin.,Liu, Guo-qiang.,Wei, Rui.,Hou, Wen-fang.,Gao, Mei-juan.,...&Hong, Tian-pei.(2011).Ghrelin promotes differentiation of human embryonic stem cells into cardiomyocytes.ACTA PHARMACOLOGICA SINICA,32(10),1239-1245.
MLA Yang, Jin,et al."Ghrelin promotes differentiation of human embryonic stem cells into cardiomyocytes".ACTA PHARMACOLOGICA SINICA 32.10(2011):1239-1245.
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