北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 生理学与病理生理学系  > 期刊论文
学科主题: 基础医学
题名:
Kidney regeneration by non-platelet RNA-containing particle-derived cells
作者: Kong, Wuyi1; Nuo, Mu1; Zhu, Xiao Ping1; Han, Xiu Juan1; Wang, Xian2
关键词: acute ischaemia ; DEAD box polypeptide 4 ; glomerulus ; kidney tubules ; non-platelet RNA-containing particles ; octamer binding transcription factor 4 ; sex-determining region Y 2 ; stem cells
刊名: CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
发表日期: 2013-11-01
DOI: 10.1111/1440-1681.12164
卷: 40, 期:11, 页:724-734
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy ; Physiology
研究领域[WOS]: Pharmacology & Pharmacy ; Physiology
关键词[WOS]: HEMATOPOIETIC STEM-CELLS ; BONE-MARROW ; PULMONARY-HYPERTENSION ; PURKINJE NEURONS ; STROMAL CELLS ; IN-VIVO ; FUSION ; TRANSDIFFERENTIATION ; DIFFERENTIATION ; CARDIOMYOCYTES
英文摘要:

We found a group of non-platelet RNA-containing particles (NPRCPs) in human umbilical cord blood. These particles can aggregate, fuse and become non-nucleated cells when cocultured with nucleated cells in vitro. The non-nucleated cells further differentiate into nucleated cells expressing octamer binding transcription factor 4 (OCT4). The NPRCPs are approximately 1-5m in diameter, have a thin bilayer membrane, contain short RNAs and microRNAs and express OCT4, sex-determining region Y 2 (SOX2) and DEAD box polypeptide 4 (DDX4). To confirm the function of NPRCPs in vivo, we examined the effects of tail vein-injected green fluorescent protein (GFP)-labelled NPRCPs on mouse kidneys damaged by prior ischaemia and reperfusion from Day 1 to Week 6. Within 1day of injection of NPRCPs, immunofluorescence and immunohistochemistry revealed a large number of extravasated NPRCPs in the renal calyces, damaged glomeruli and duct tubules. During the course of regeneration, NPRCPs fused into large, non-nucleated cellular structures that further became large nucleated cells to regenerate multicellular kidney tubules. In addition, many NPRCPs became tiny nucleated cellular structures that further differentiated into interstitial cells in connective tissue. The extravasated NPRCPs also arranged themselves into non-cell glomerular structures before further regenerating into nucleated cells of the glomerulus. In conclusion, the results demonstrate that, via different patterns of differentiation, NPRCP-derived cells can regenerate mouse kidney tissue damaged by ischaemia.

语种: 英语
项目资助者: Department of Technology, Inner Mongolia Government, China
WOS记录号: WOS:000326072000002
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64627
Appears in Collections:基础医学院_生理学与病理生理学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Kidney regeneration by non-platelet RNA-containing particle-derived cells.pdf(4529KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Beijing Univ, Beijing Khasar Med Technol, Beijing 100871, Peoples R China
2.Beijing Univ, Dept Physiol, Beijing 100871, Peoples R China

Recommended Citation:
Kong, Wuyi,Nuo, Mu,Zhu, Xiao Ping,et al. Kidney regeneration by non-platelet RNA-containing particle-derived cells[J]. CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY,2013,40(11):724-734.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Kong, Wuyi]'s Articles
[Nuo, Mu]'s Articles
[Zhu, Xiao Ping]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Kong, Wuyi]‘s Articles
[Nuo, Mu]‘s Articles
[Zhu, Xiao Ping]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace