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学科主题: 临床医学
题名:
Expression of Th-1 chemokines and chemokine receptors on the ocular surface of C57BL/6 mice: Effects of desiccating stress
作者: Yoon, Kyung-Chul; De Paiva, Cintia S.; Qi, Hong; Chen, Zhuo; Farley, William J.; Li, De-Quan; Pflugfelder, Stephen C.
刊名: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
发表日期: 2007-06-01
DOI: 10.1167/iovs.07-0002
卷: 48, 期:6, 页:2561-2569
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Ophthalmology
研究领域[WOS]: Ophthalmology
关键词[WOS]: EXPERIMENTAL DRY EYE ; CORNEAL EPITHELIAL-CELLS ; CONJUNCTIVAL EPITHELIUM ; SJOGRENS-SYNDROME ; TEAR FLUID ; KERATOCONJUNCTIVITIS SICCA ; MATRIX METALLOPROTEINASES ; CYTOKINE EXPRESSION ; BARRIER DISRUPTION ; GENE-EXPRESSION
英文摘要:

PURPOSE. To evaluate the effects of desiccating ocular surface stress on the expression of chemokines and their receptors by the corneal epithelium and conjunctiva of C57BL/6 and BALB/c mice.

METHODS. Experimental dry eye was created in C57BL/6 and BALB/c mice. The concentrations of macrophage inflammatory protein 1 alpha (MIP-1 alpha), MIP-1 beta, monokine induced by interferon (MIG)-gamma, and interferon-gamma-inducible protein (IP)-10 in the corneal epithelia and conjunctiva were measured by a multiplex immunobead assay. Expression of MIP-1 alpha; MIP-1 beta; regulated on activation, normal T-cell expressed and secreted (RANTES), MIG, IP-10; monocyte chemoattractant protein (MCP)-3; eotaxin-1; CCR5; CXCR3; and CCR3 in the cornea and conjunctiva were evaluated by real-time PCR and immunostaining.

RESULTS. Desiccating stress significantly increased concentrations of MIP-la, MIP-10, IP-10, and MIG proteins in the corneal epithelium and conjunctiva of C57BL/6 mice. Furthermore, it increased levels of MIP-la, MIP-1 beta, and CCR5 transcripts in the cornea and conjunctiva and RANTES, MIG, IP-10, and CXCR3 transcripts in the conjunctiva of C57BL/6 mice. In contrast, levels of MCP-3, eotaxin-1, and CCR3 transcripts increased in both tissues of BALB/c mice. In situ immunodetection of chemokines and their receptors was similar to their pattern of gene expression.

CONCLUSIONS. Specific patterns of Th-1 and -2 chemokines and their receptors are induced in the mouse ocular surface by desiccating stress in a strain-related fashion. Desiccating stress potently stimulates the expression of Th-1 cell-attracting chemokines and chemokine receptors on the ocular surface of C57BL/6 mice.

语种: 英语
WOS记录号: WOS:000246967100014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64639
Appears in Collections:北京大学第三临床医学院_眼科_期刊论文

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作者单位: 1.Peking Univ, Ctr Eye, Hosp 3, Beijing 100871, Peoples R China
2.Baylor Univ, Cullen Eye Inst, Dept Ophthalmol, Ocular Surface Ctr, Houston, TX 77030 USA
3.Chonnam Natl Univ, Dept Ophthalmol, Med Sch & Hosp, Kwangju, South Korea

Recommended Citation:
Yoon, Kyung-Chul,De Paiva, Cintia S.,Qi, Hong,et al. Expression of Th-1 chemokines and chemokine receptors on the ocular surface of C57BL/6 mice: Effects of desiccating stress[J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,2007,48(6):2561-2569.
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