IR@PKUHSC  > 北京大学第三临床医学院  > 眼科
学科主题临床医学
Expression of Th-1 chemokines and chemokine receptors on the ocular surface of C57BL/6 mice: Effects of desiccating stress
Yoon, Kyung-Chul; De Paiva, Cintia S.; Qi, Hong; Chen, Zhuo; Farley, William J.; Li, De-Quan; Pflugfelder, Stephen C.
刊名INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
2007-06-01
DOI10.1167/iovs.07-0002
48期:6页:2561-2569
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]EXPERIMENTAL DRY EYE ; CORNEAL EPITHELIAL-CELLS ; CONJUNCTIVAL EPITHELIUM ; SJOGRENS-SYNDROME ; TEAR FLUID ; KERATOCONJUNCTIVITIS SICCA ; MATRIX METALLOPROTEINASES ; CYTOKINE EXPRESSION ; BARRIER DISRUPTION ; GENE-EXPRESSION
英文摘要

PURPOSE. To evaluate the effects of desiccating ocular surface stress on the expression of chemokines and their receptors by the corneal epithelium and conjunctiva of C57BL/6 and BALB/c mice.

METHODS. Experimental dry eye was created in C57BL/6 and BALB/c mice. The concentrations of macrophage inflammatory protein 1 alpha (MIP-1 alpha), MIP-1 beta, monokine induced by interferon (MIG)-gamma, and interferon-gamma-inducible protein (IP)-10 in the corneal epithelia and conjunctiva were measured by a multiplex immunobead assay. Expression of MIP-1 alpha; MIP-1 beta; regulated on activation, normal T-cell expressed and secreted (RANTES), MIG, IP-10; monocyte chemoattractant protein (MCP)-3; eotaxin-1; CCR5; CXCR3; and CCR3 in the cornea and conjunctiva were evaluated by real-time PCR and immunostaining.

RESULTS. Desiccating stress significantly increased concentrations of MIP-la, MIP-10, IP-10, and MIG proteins in the corneal epithelium and conjunctiva of C57BL/6 mice. Furthermore, it increased levels of MIP-la, MIP-1 beta, and CCR5 transcripts in the cornea and conjunctiva and RANTES, MIG, IP-10, and CXCR3 transcripts in the conjunctiva of C57BL/6 mice. In contrast, levels of MCP-3, eotaxin-1, and CCR3 transcripts increased in both tissues of BALB/c mice. In situ immunodetection of chemokines and their receptors was similar to their pattern of gene expression.

CONCLUSIONS. Specific patterns of Th-1 and -2 chemokines and their receptors are induced in the mouse ocular surface by desiccating stress in a strain-related fashion. Desiccating stress potently stimulates the expression of Th-1 cell-attracting chemokines and chemokine receptors on the ocular surface of C57BL/6 mice.

语种英语
WOS记录号WOS:000246967100014
引用统计
被引频次:98[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64639
专题北京大学第三临床医学院_眼科
作者单位1.Peking Univ, Ctr Eye, Hosp 3, Beijing 100871, Peoples R China
2.Baylor Univ, Cullen Eye Inst, Dept Ophthalmol, Ocular Surface Ctr, Houston, TX 77030 USA
3.Chonnam Natl Univ, Dept Ophthalmol, Med Sch & Hosp, Kwangju, South Korea
推荐引用方式
GB/T 7714
Yoon, Kyung-Chul,De Paiva, Cintia S.,Qi, Hong,et al. Expression of Th-1 chemokines and chemokine receptors on the ocular surface of C57BL/6 mice: Effects of desiccating stress[J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,2007,48(6):2561-2569.
APA Yoon, Kyung-Chul.,De Paiva, Cintia S..,Qi, Hong.,Chen, Zhuo.,Farley, William J..,...&Pflugfelder, Stephen C..(2007).Expression of Th-1 chemokines and chemokine receptors on the ocular surface of C57BL/6 mice: Effects of desiccating stress.INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,48(6),2561-2569.
MLA Yoon, Kyung-Chul,et al."Expression of Th-1 chemokines and chemokine receptors on the ocular surface of C57BL/6 mice: Effects of desiccating stress".INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 48.6(2007):2561-2569.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yoon, Kyung-Chul]的文章
[De Paiva, Cintia S.]的文章
[Qi, Hong]的文章
百度学术
百度学术中相似的文章
[Yoon, Kyung-Chul]的文章
[De Paiva, Cintia S.]的文章
[Qi, Hong]的文章
必应学术
必应学术中相似的文章
[Yoon, Kyung-Chul]的文章
[De Paiva, Cintia S.]的文章
[Qi, Hong]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。