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学科主题: 基础医学
题名:
The role of human ADA2a in the regulation of p53 acetylation and stability
作者: Huang Jing1; Zhang Li1; Xiao Lin1; Xu LanJun1; Hu FanLei1; Shao WenWei1; Liu Wei1; Mo XiaoNing1; Shi TaiPing1,2; Qiu XiaoYan1
关键词: hADA2a ; p53 ; acetylation ; PCAF
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2011-02-01
DOI: 10.1007/s11434-010-4311-0
卷: 56, 期:4-5, 页:397-405
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: ACIDIC ACTIVATION DOMAINS ; DNA-DAMAGE ; TRANSCRIPTIONAL ADAPTER ; GCN5 ; COMPLEXES ; PROTEIN ; GENE ; HOMOLOGS ; BINDING ; GROWTH
英文摘要:

The tumor suppressor protein p53 is a well-known transcription factor that functions as a critical component of the genotoxic stress response via regulating the expression of effector proteins that control cellular fate following DNA damage. The human p300/CBP-associated factor (PCAF)-containing histone acetyltransferase (HAT) complex is important for the stability and activity of p53 via its acetylation. The human homolog of yeast alteration/deficiency in activation 2a (ADA2a) is a stable component of the human PCAF-containing HAT complex. In this study, we demonstrated that p53 and hADA2a physically interact with each other in human HEK 293T cells. Using overexpression and small interfering RNA-mediated knockdown, we demonstrated that hADA2a stabilizes p53 via promoting its acetylation at lysine 320 - a PCAF-dependent acetylation site. Furthermore, hADA2a can potentiate the transcriptional activity of p53 at the BAX and p21 promoters to induce cell apoptosis and cell cycle arrest. Overall, our results establish that hADA2a, a component of the PCAF-containing histone acetyltransferase co-activator complex, is a mediator of acetylation-dependent stabilization and activation of p53 in mammalian cells.

语种: 英语
所属项目编号: 2009ZX09503-004 ; 30901303 ; BMU20090459
项目资助者: Ministry of Science and Technology for Drug Development ; National Natural Sciences Foundation of China ; Foundation for New Youth Scholars of Peking University Health Science Center
WOS记录号: WOS:000287853100008
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64649
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Chinese Natl Human Genome Ctr, Beijing 100176, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Lab Med Immunol, Beijing 100191, Peoples R China

Recommended Citation:
Huang Jing,Zhang Li,Xiao Lin,et al. The role of human ADA2a in the regulation of p53 acetylation and stability[J]. CHINESE SCIENCE BULLETIN,2011,56(4-5):397-405.
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