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学科主题: 药学
题名:
Hepatocytes targeting of cationic liposomes modified with soybean sterylglucoside and polyethylene glycol
作者: Qi, Xian-Rong; Yan, Wen-Wei; Shi, Jing
关键词: Hepatocytes targeting ; Cationic liposomes ; Soybean sterylglucoside
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2005-08-28
卷: 11, 期:32, 页:4947-4952
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: HEPATITIS-B ; ANTISENSE OLIGODEOXYNUCLEOTIDES ; GENE-EXPRESSION ; DIPALMITOYLPHOSPHATIDYLCHOLINE LIPOSOMES ; IN-VITRO ; DELIVERY ; DNA ; INHIBITION ; MIXTURE ; LIGANDS
英文摘要:

AIM: In this study, a hepatocyte-specific targeting technology was developed by modifying cationic liposomes with soybean sterylglucoside (SG) and polyethylene glycol (PEG) (C/SG/PEG-liposomes).

METHODS: The liposomal transfection efficiencies in HepG(2) 2.2.15 cells were estimated with the use of fluorescein sodium (FS) as a model drug, by flow cytometry. The antisense activity of C/SG/PEG-liposomes entrapped antisense oligonucleotides (ODN) was determined as HBsAg and HBeAg in HepG(2) 2.2.15 cells by ELISA. The liposome uptake by liver and liver cells in mice was carried out after intravenous injection of H-3-labeled liposomes.

RESULTS: C/SG-liposomes entrapped FS were effectively transfected into HepG(2) 2.2.15 cells in vitro. C/SG/PEG-liposomes entrapped ODN, reduced the secretion of both HBsAg and HBeAg in HepG(2) 2.2.15 cells when compared to free ODN. After in vivo injection of H-3-labeled C/SG/PEG-liposomes, higher radiation accumulation was observed in the hepatocytes than non-parenchymal cells of the liver.

CONCLUSION: C/SG/PEG-liposomes mediated gene transfer to the liver is an effective gene-delivery method for hepatocytes-specific targeting, which appears to have a potential for gene therapy of HBV infections. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 30371265
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000208099600003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64660
Appears in Collections:北京大学药学院_药剂学系_期刊论文

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作者单位: Peking Univ, Dept Pharmaceut, Sch Pharmaceut Sci, Beijing 100083, Peoples R China

Recommended Citation:
Qi, Xian-Rong,Yan, Wen-Wei,Shi, Jing. Hepatocytes targeting of cationic liposomes modified with soybean sterylglucoside and polyethylene glycol[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2005,11(32):4947-4952.
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