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Hepatocytes targeting of cationic liposomes modified with soybean sterylglucoside and polyethylene glycol
Qi, Xian-Rong; Yan, Wen-Wei; Shi, Jing
关键词Hepatocytes targeting Cationic liposomes Soybean sterylglucoside
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2005-08-28
11期:32页:4947-4952
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]HEPATITIS-B ; ANTISENSE OLIGODEOXYNUCLEOTIDES ; GENE-EXPRESSION ; DIPALMITOYLPHOSPHATIDYLCHOLINE LIPOSOMES ; IN-VITRO ; DELIVERY ; DNA ; INHIBITION ; MIXTURE ; LIGANDS
英文摘要

AIM: In this study, a hepatocyte-specific targeting technology was developed by modifying cationic liposomes with soybean sterylglucoside (SG) and polyethylene glycol (PEG) (C/SG/PEG-liposomes).

METHODS: The liposomal transfection efficiencies in HepG(2) 2.2.15 cells were estimated with the use of fluorescein sodium (FS) as a model drug, by flow cytometry. The antisense activity of C/SG/PEG-liposomes entrapped antisense oligonucleotides (ODN) was determined as HBsAg and HBeAg in HepG(2) 2.2.15 cells by ELISA. The liposome uptake by liver and liver cells in mice was carried out after intravenous injection of H-3-labeled liposomes.

RESULTS: C/SG-liposomes entrapped FS were effectively transfected into HepG(2) 2.2.15 cells in vitro. C/SG/PEG-liposomes entrapped ODN, reduced the secretion of both HBsAg and HBeAg in HepG(2) 2.2.15 cells when compared to free ODN. After in vivo injection of H-3-labeled C/SG/PEG-liposomes, higher radiation accumulation was observed in the hepatocytes than non-parenchymal cells of the liver.

CONCLUSION: C/SG/PEG-liposomes mediated gene transfer to the liver is an effective gene-delivery method for hepatocytes-specific targeting, which appears to have a potential for gene therapy of HBV infections. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000208099600003
项目编号30371265
资助机构National Natural Science Foundation of China
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64660
专题北京大学药学院_药剂学系
北京大学第三临床医学院_动物实验中心
作者单位Peking Univ, Dept Pharmaceut, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Qi, Xian-Rong,Yan, Wen-Wei,Shi, Jing. Hepatocytes targeting of cationic liposomes modified with soybean sterylglucoside and polyethylene glycol[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2005,11(32):4947-4952.
APA Qi, Xian-Rong,Yan, Wen-Wei,&Shi, Jing.(2005).Hepatocytes targeting of cationic liposomes modified with soybean sterylglucoside and polyethylene glycol.WORLD JOURNAL OF GASTROENTEROLOGY,11(32),4947-4952.
MLA Qi, Xian-Rong,et al."Hepatocytes targeting of cationic liposomes modified with soybean sterylglucoside and polyethylene glycol".WORLD JOURNAL OF GASTROENTEROLOGY 11.32(2005):4947-4952.
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