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学科主题临床医学
Human Embryonic Stem Cells - a Potential Vaccine for Ovarian Cancer
Zhang, Zu-Juan; Chen, Xin-Hua; Chang, Xiao-Hong; Ye, Xue; Li, Yi; Cui, Heng
关键词Ovarian cancer human embryonic stem cell antitumor immunity cancer vaccine
刊名ASIAN PACIFIC JOURNAL OF CANCER PREVENTION
2012
DOI10.7314/APJCP.2012.13.9.4295
13期:9页:4295-4300
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
资助者National Natural Science Foundation of China ; Research and Development foundation in Peking University People&prime ; s Hospital ; National Natural Science Foundation of China ; Research and Development foundation in Peking University People&prime ; s Hospital
研究领域[WOS]Oncology
关键词[WOS]ANTIGENS ; IMMUNOTHERAPY ; EXPRESSION ; RELEVANCE ; IMMUNITY ; BIOLOGY ; MOUSE ; MODEL
英文摘要

Objective: To investigate the therapeutic potential of human embryonic stem cells (hESCs) as a vaccine to induce an immune response and provide antitumor protection in a rat model. Methods: Cross-reactivity of antigens between hESCs and tumour cells was screened by immunohistochemistry. Fischer 344 rats were divided into 7 groups, with 6 rats in each, immunized with: Group 1, hESC; Group 2, pre-inactivated mitotic NuTu-19; Group 3 PBS; Group 4, hESC; Group 5, pre-inactivated mitotic NuTu-19; Group 6, PBS; Group 7, hESC only. At 1 (Groups 1-3) or 4 weeks (Groups 4-6) after the last vaccination, each rat was challenged intraperitoneally with NuTu-19. Tumor growth and animal survival were closely monitored. Rats immunized with H9 and NuTu-19 were tested by Western blot analysis of rat orbital venous blood for cytokines produced by Th1 and Th2 cells. Results: hESCs presented tumour antigens, markers, and genes related to tumour growth, metastasis, and signal pathway interactions. The vaccine administered to rats in Group 1 led to significant antitumor responses and enhanced tumor rejection in rats with intraperitoneal inoculation of NuTu-19 cells compared to control groups. In contrast, rats in Group 4 did not display any elevation of antitumour responses. Western blot analysis found cross-reactivity among antibodies generated between H9 and NuTu-19. However, the cytokines did not show significant differences, and no side effects were detected. Conclusion: hESC-based vaccination is a promising modality for immunotherapy of ovarian cancer.

语种英语
所属项目编号81072141 ; RDB2010-09
资助者National Natural Science Foundation of China ; Research and Development foundation in Peking University People&prime ; s Hospital ; National Natural Science Foundation of China ; Research and Development foundation in Peking University People&prime ; s Hospital
WOS记录号WOS:000312267800015
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64666
专题北京大学第二临床医学院_妇科肿瘤中心
作者单位Peking Univ, Peoples Hosp, Gynecol Oncol Ctr, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Zu-Juan,Chen, Xin-Hua,Chang, Xiao-Hong,et al. Human Embryonic Stem Cells - a Potential Vaccine for Ovarian Cancer[J]. ASIAN PACIFIC JOURNAL OF CANCER PREVENTION,2012,13(9):4295-4300.
APA Zhang, Zu-Juan,Chen, Xin-Hua,Chang, Xiao-Hong,Ye, Xue,Li, Yi,&Cui, Heng.(2012).Human Embryonic Stem Cells - a Potential Vaccine for Ovarian Cancer.ASIAN PACIFIC JOURNAL OF CANCER PREVENTION,13(9),4295-4300.
MLA Zhang, Zu-Juan,et al."Human Embryonic Stem Cells - a Potential Vaccine for Ovarian Cancer".ASIAN PACIFIC JOURNAL OF CANCER PREVENTION 13.9(2012):4295-4300.
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