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学科主题: 口腔医学
题名:
Role of Polymorphonuclear Neutrophils in the Clearance of Enterococcus faecalis Derived from Saliva and Infected Root Canals
作者: Ma, Zeyun2; Wang, Yixiang3; Zhu, Xiaofei2; Zhang, Chengfei1,2; Li, Shenglin3; Jin, Lijian; Shen, Ya4; Haapasalo, Markus4
关键词: Enterococcus faecalis ; immunostaining ; inflammatory cytokines ; polymorphonuclear neutrophils
刊名: JOURNAL OF ENDODONTICS
发表日期: 2011-03-01
DOI: 10.1016/j.joen.2010.11.033
卷: 37, 期:3, 页:346-352
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Dentistry, Oral Surgery & Medicine
关键词[WOS]: MATRIX METALLOPROTEINASE-8 ; ENDODONTIC FAILURE ; VIRULENCE FACTORS ; CATHEPSIN-G ; PERIODONTITIS ; ELASTASE ; SUSCEPTIBILITY ; MICROORGANISMS ; INFLAMMATION ; COMPLEMENT
英文摘要:

Objectives: The goal of this study was to measure (1) the ability of polymorphonuclear neutrophil leukocytes (PMNs) to kill oral Enterococcus faecalis strains, (2) up-regulation of inflammatory mediators by PMNs in interaction with E. faecalis, and (3) the ability of E. faecalis to cause inflammation in mouse muscle tissue. Methods: Fifteen endodontic and nine saliva strains of E. faecalis were isolated and identified by specific 165 ribosomal RNA (165 rRNA) primers. The bacteria were grown in BHI broth and incubated with mouse PMN in appropriate media to determine the ability of the PMNs to kill the bacteria. In other experiments up-regulation of interleukin (IL)-1 alpha, tumor necrosis factor alpha (TNF-alpha), matrix metalloproteinase-8 (MMP-8), and cyclooxygenase (COX)-2 messenger RNA in the PMNs was measured after exposure of the leukocytes to the bacteria using real-time polymerase chain reaction. Finally, the inflammatory potential of and PMN response to E. faecalis suspension in mouse muscle tissue was examined from histological sections using hematoxylin-eosin staining and immunostaining. Results: Murine PMNs killed about 80% of the E. faecalis cells in 1 hour, irrespective of the source of isolation of the strains. Quantitative PCR results showed that IL-1 alpha, TNF-alpha, MMP-8, and COX-2 messenger RNA were markedly up-regulated in E. faecalis stimulated PMNs or in E. faecalis invaded muscular tissues. MMP-8 messenger RNA level was positively related to COX-2 messenger RNA level. Histological evaluation and immunostaining disclosed that all E. faecalis strains could recruit PMNs to the local infectious sites and cause abscess formation. Conclusion: E. faecalis strains from saliva and infected root canals have the potential to recruit PMNs in the infectious sites leading to inflammation via up-regulation of PMN IL-1 alpha, TNF-alpha, MMP-8, and COX-2. PMNs can play an important role in killing of E. faecalis. (J Endod 2011;37:346-352)

语种: 英语
所属项目编号: 2009DFA32950
项目资助者: China International Science and Technology Cooperation Grant
WOS记录号: WOS:000288290000009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64668
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Univ Hong Kong, Fac Dent, Area Endodont, Hong Kong, Hong Kong, Peoples R China
2.Peking Univ Sch & Hosp Stomatol, Dept Special Dent, Beijing, Peoples R China
3.Peking Univ Sch & Hosp Stomatol, Cent Lab, Beijing, Peoples R China
4.Univ British Columbia, Fac Dent, Dept Oral Biol & Med Sci, Div Endodont, Vancouver, BC V5Z 1M9, Canada

Recommended Citation:
Ma, Zeyun,Wang, Yixiang,Zhu, Xiaofei,et al. Role of Polymorphonuclear Neutrophils in the Clearance of Enterococcus faecalis Derived from Saliva and Infected Root Canals[J]. JOURNAL OF ENDODONTICS,2011,37(3):346-352.
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