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学科主题口腔医学
Role of Polymorphonuclear Neutrophils in the Clearance of Enterococcus faecalis Derived from Saliva and Infected Root Canals
Ma, Zeyun2; Wang, Yixiang3; Zhu, Xiaofei2; Zhang, Chengfei1,2; Li, Shenglin3; Jin, Lijian; Shen, Ya4; Haapasalo, Markus4
关键词Enterococcus faecalis immunostaining inflammatory cytokines polymorphonuclear neutrophils
刊名JOURNAL OF ENDODONTICS
2011-03-01
DOI10.1016/j.joen.2010.11.033
37期:3页:346-352
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]MATRIX METALLOPROTEINASE-8 ; ENDODONTIC FAILURE ; VIRULENCE FACTORS ; CATHEPSIN-G ; PERIODONTITIS ; ELASTASE ; SUSCEPTIBILITY ; MICROORGANISMS ; INFLAMMATION ; COMPLEMENT
英文摘要

Objectives: The goal of this study was to measure (1) the ability of polymorphonuclear neutrophil leukocytes (PMNs) to kill oral Enterococcus faecalis strains, (2) up-regulation of inflammatory mediators by PMNs in interaction with E. faecalis, and (3) the ability of E. faecalis to cause inflammation in mouse muscle tissue. Methods: Fifteen endodontic and nine saliva strains of E. faecalis were isolated and identified by specific 165 ribosomal RNA (165 rRNA) primers. The bacteria were grown in BHI broth and incubated with mouse PMN in appropriate media to determine the ability of the PMNs to kill the bacteria. In other experiments up-regulation of interleukin (IL)-1 alpha, tumor necrosis factor alpha (TNF-alpha), matrix metalloproteinase-8 (MMP-8), and cyclooxygenase (COX)-2 messenger RNA in the PMNs was measured after exposure of the leukocytes to the bacteria using real-time polymerase chain reaction. Finally, the inflammatory potential of and PMN response to E. faecalis suspension in mouse muscle tissue was examined from histological sections using hematoxylin-eosin staining and immunostaining. Results: Murine PMNs killed about 80% of the E. faecalis cells in 1 hour, irrespective of the source of isolation of the strains. Quantitative PCR results showed that IL-1 alpha, TNF-alpha, MMP-8, and COX-2 messenger RNA were markedly up-regulated in E. faecalis stimulated PMNs or in E. faecalis invaded muscular tissues. MMP-8 messenger RNA level was positively related to COX-2 messenger RNA level. Histological evaluation and immunostaining disclosed that all E. faecalis strains could recruit PMNs to the local infectious sites and cause abscess formation. Conclusion: E. faecalis strains from saliva and infected root canals have the potential to recruit PMNs in the infectious sites leading to inflammation via up-regulation of PMN IL-1 alpha, TNF-alpha, MMP-8, and COX-2. PMNs can play an important role in killing of E. faecalis. (J Endod 2011;37:346-352)

语种英语
WOS记录号WOS:000288290000009
项目编号2009DFA32950
资助机构China International Science and Technology Cooperation Grant
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64668
专题北京大学口腔医学院
北京大学口腔医学院_特诊科
北京大学口腔医学院_中心实验室
作者单位1.Univ Hong Kong, Fac Dent, Area Endodont, Hong Kong, Hong Kong, Peoples R China
2.Peking Univ Sch & Hosp Stomatol, Dept Special Dent, Beijing, Peoples R China
3.Peking Univ Sch & Hosp Stomatol, Cent Lab, Beijing, Peoples R China
4.Univ British Columbia, Fac Dent, Dept Oral Biol & Med Sci, Div Endodont, Vancouver, BC V5Z 1M9, Canada
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GB/T 7714
Ma, Zeyun,Wang, Yixiang,Zhu, Xiaofei,et al. Role of Polymorphonuclear Neutrophils in the Clearance of Enterococcus faecalis Derived from Saliva and Infected Root Canals[J]. JOURNAL OF ENDODONTICS,2011,37(3):346-352.
APA Ma, Zeyun.,Wang, Yixiang.,Zhu, Xiaofei.,Zhang, Chengfei.,Li, Shenglin.,...&Haapasalo, Markus.(2011).Role of Polymorphonuclear Neutrophils in the Clearance of Enterococcus faecalis Derived from Saliva and Infected Root Canals.JOURNAL OF ENDODONTICS,37(3),346-352.
MLA Ma, Zeyun,et al."Role of Polymorphonuclear Neutrophils in the Clearance of Enterococcus faecalis Derived from Saliva and Infected Root Canals".JOURNAL OF ENDODONTICS 37.3(2011):346-352.
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