IR@PKUHSC  > 北京大学基础医学院  > 药理学系
学科主题基础医学
Differential expression of genes and changes in glucose metabolism in the liver of liver-specific glucokinase gene knockout mice
Wang, Rong1; Gao, Hui1; Xu, Wei1; Li, Hui1; Mao, Yiqing1; Wang, Yi1; Guo, Tingting1; Wang, Xi1; Song, Rongjing1; Li, Zhixin2; Irwin, David M.1,3; Niu, Gang4; Tan, Huanran1
关键词Glucokinase (GCK) MODY2 Differentially expressed genes Liver
刊名GENE
2013-03-10
DOI10.1016/j.gene.2012.12.036
516期:2页:248-254
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Genetics & Heredity
研究领域[WOS]Genetics & Heredity
关键词[WOS]DEPENDENT DIABETES-MELLITUS ; HEPATIC GLYCOGEN-SYNTHESIS ; BETA-CELL GLUCOKINASE ; PHOSPHOENOLPYRUVATE CARBOXYKINASE ; CHOLESTEROL ACYLTRANSFERASE ; NEONATAL-RAT ; ANIMAL-MODEL ; INSULIN ; MUTATIONS ; YOUNG
英文摘要

To investigate the role of liver-specific expression of glucokinase (GCK) in the pathogenesis of hyperglycemia and to identify candidate genes involved in mechanisms of the onset and progression of maturity onset diabetes of the young, type 2 (MODY-2), we examined changes in biochemical parameters and gene expression in GCK knockout (gck(w/-)) and wild-type (gck(w/w)) mice as they aged. Fasting blood glucose levels were found to be significantly higher in the gck(w/-) mice, compared to age-matched gck(w/w) mice, at all ages (P<0.05), except at 2 weeks. GCK activity of gck(w/-) mice was about 50% of that of wild type (gck(w/w)) mice (P<0.05). Glycogen content at 4 and 40 weeks of age was lower in gck(w/-) mice compared to gck(w/w) mice. Differentially expressed genes in the livers of 2 and 26 week-old liver-specific GCK knockout (gck(w/-)) mice were identified by suppression subtractive hybridization (SSH), which resulted in the identification of phosphoenolpyruvatecarboxykinase (PEPCK, also called PCK1) and Sterol O-acyltransferase 2 (SOAT2) as candidate genes involved in pathogenesis. The expressions of PEPCK and SOAT2 along with glycogen phosphorylase (GP) and glycogen synthase (GS) were then examined in GCK knockout (gck(w/-)) and wild-type (gck(w/w)) mice at different ages. Changes in PEPCK mRNA levels were confirmed by real-time RT-PCR, while no differences in the levels of expression of SOAT2 or GS were observed in age-matched GCK knockout (gck(w/-)) and wild-type (gck(w/w)) mice. GP mRNA levels were decreased in 40-week old gck(w/-) mice compared to age-matched gck(w/w) mice. Changes in gluconeogenesis, delayed development of GCK and impaired hepatic glycogen synthesis in the liver potentially lead to the onset and progression of MODY2. (C) 2012 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000315430900008
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64669
专题北京大学基础医学院_药理学系
北京大学基础医学院
北京大学第一临床医学院_检验科
北京大学第二临床医学院_药剂科
北京大学第三临床医学院_骨科
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Pharmacol, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Integrated Tradit Chinese & Western Med, Beijing 100191, Peoples R China
3.Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON M5S 1A8, Canada
4.Beijing N&N Genetech Co, Beijing 100082, Peoples R China
推荐引用方式
GB/T 7714
Wang, Rong,Gao, Hui,Xu, Wei,et al. Differential expression of genes and changes in glucose metabolism in the liver of liver-specific glucokinase gene knockout mice[J]. GENE,2013,516(2):248-254.
APA Wang, Rong.,Gao, Hui.,Xu, Wei.,Li, Hui.,Mao, Yiqing.,...&Tan, Huanran.(2013).Differential expression of genes and changes in glucose metabolism in the liver of liver-specific glucokinase gene knockout mice.GENE,516(2),248-254.
MLA Wang, Rong,et al."Differential expression of genes and changes in glucose metabolism in the liver of liver-specific glucokinase gene knockout mice".GENE 516.2(2013):248-254.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, Rong]的文章
[Gao, Hui]的文章
[Xu, Wei]的文章
百度学术
百度学术中相似的文章
[Wang, Rong]的文章
[Gao, Hui]的文章
[Xu, Wei]的文章
必应学术
必应学术中相似的文章
[Wang, Rong]的文章
[Gao, Hui]的文章
[Xu, Wei]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。