|Sulfur dioxide derivatives depress L-type calcium channel in rat cardiomyocytes|
|Zhang, Rong-Yuan1,5; Du, Jun-Bao1,4,5; Sun, Yan1; Chen, Stella6; Tsai, Hao-Jan2; Yuan, Lan5; Li, Lin2; Tang, Chao-Shu3,4; Jin, Hong-Fang1|
|关键词||cardiomyocytes confocal microscopy intracellular calcium L-type calcium channels sulfur dioxide whole-cell patch-clamp|
|刊名||CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Pharmacology & Pharmacy ; Physiology|
|研究领域[WOS]||Pharmacology & Pharmacy ; Physiology|
|关键词[WOS]||CARDIAC MYOCYTES ; BLOOD-PRESSURE ; HEART|
1. Sulfur dioxide (SO(2)) has recently been found to have various biological effects on the cardiovascular system. The present study was designed to explore the effects of SO(2) derivatives on the L-type calcium current (I (Ca, L)) in isolated rat ventricular cardiomyocytes.
2. A Langendorf system was used to dissociate single ventricular cells. SO(2) derivatives from 5 to 1000 mu mol/L were incubated with cardiomyocytes. The whole-cell patch-clamp technique was used to record I (Ca, L). The effect of SO(2) derivatives on intracellular calcium concentration ([Ca(2+)](i)) was detected by confocal microscopy.
3. Concentrations of 5 or 10 mu mol/L SO(2) derivatives could not change I (Ca, L) evoked by a single pulse from -40 to 0 mV for 200 ms in rat ventricular cardiomyocytes; however, 50, 100, 500 or 1000 mu mol/L SO(2) derivatives could depress the peak amplitudes of calcium currents in 6 min, and the I (Ca, L) was attenuated by 13.19%, 16.59%, 21.23% and 24.72%, respectively, as compared with corresponding controls (P < 0.05). The 50, 100, 500 or 1000 mu mol/L SO(2) derivatives also depressed the peak I-V curves, without altering the reversal potential and the voltage dependence of the peak I (Ca, L). Therefore, 1000 lmol/L SO(2) derivatives could reduce [Ca(2+)](i) in cardiomyocytes.
4. The results of the present study suggest that SO(2) derivatives can depress I (Ca, L) in cardiomyocytes, which might have a protective effect in cardiovascular diseases.
|项目编号||2011CB503904 ; 30821001 ; 81070111 ; 7112130 ; 7082095|
|资助机构||Major Basic Research Program of China ; National Natural Science Foundation of China ; Beijing Natural Science Foundation|
|作者单位||1.Peking Univ, Dept Pediat, Hosp 1, Beijing 100034, Peoples R China|
2.Peking Univ, Dept Cent Lab, Hosp 1, Beijing 100034, Peoples R China
3.Peking Univ, Inst Cardiovasc Res, Hosp 1, Beijing 100034, Peoples R China
4.Peking Univ, Key Lab Mol Cardiovasc Dis, Minist Educ, Beijing 100034, Peoples R China
5.Peking Univ, Lab Laser Scanning Confocal Microscope, Hlth Sci Ctr, Beijing 100034, Peoples R China
6.Univ Calif San Diego, Dept Biochem & Cellular Biol, San Diego, CA 92103 USA
|Zhang, Rong-Yuan,Du, Jun-Bao,Sun, Yan,et al. Sulfur dioxide derivatives depress L-type calcium channel in rat cardiomyocytes[J]. CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY,2011,38(7):366-372.|
|APA||Zhang, Rong-Yuan.,Du, Jun-Bao.,Sun, Yan.,Chen, Stella.,Tsai, Hao-Jan.,...&Jin, Hong-Fang.(2011).Sulfur dioxide derivatives depress L-type calcium channel in rat cardiomyocytes.CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY,38(7),366-372.|
|MLA||Zhang, Rong-Yuan,et al."Sulfur dioxide derivatives depress L-type calcium channel in rat cardiomyocytes".CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY 38.7(2011):366-372.|