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Down-regulation of mitotic checkpoint in transformed human embryo lung fibroblasts induced by N-methyl-N-′-nitro-N-nitrosoguaridine
Yi, ZC; Zhang, M; Fu, JL; Wang, Z; Zhou, ZC
关键词cell transformation chromosome instability hBub1 gene mitotic checkpoint
刊名CHINESE MEDICAL JOURNAL
2004-07-01
117期:7页:1079-1083
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]SPINDLE CHECKPOINT ; HUMAN CANCERS ; SOLID TUMORS ; PROTEIN ; GENES ; CELL ; IDENTIFICATION ; INSTABILITY ; EXPRESSION ; MITOSIS
英文摘要

Background Mutations in mitotic checkpoint genes have been detected in several human cancers, which exhibit chromosome instability. We wanted to know whether mutation of hBub1 could occur in transformed human embryo lung fibroblasts (HELF) cells induced by a chemical carcinogen.

Methods HELF cells were transformed by N-methyl-N′-nitro-N- nitrosoguaridine ( MNNG), and three flasks of transformed HELF cells (named as T1, T2, and T3) were selected as amplifiers, and mutations of hBub1 in these transformed cells were analyzed by PCR-SSCP and sequencing.

Results It was found that any one of three transformed cell lines exhibited aneuploidy with a low mitotic checkpoint function. Subsequent PCR-SSCP and sequence analysis showed an AGT to CGT or ATT mutation at codon 80 in hBub1 gene in T1 cells with a resultant change in amino acid sequence.

Conclusion Our study demonstrated that the mitotic checkpoint genes could be targets of MNNG.

语种英语
WOS记录号WOS:000223074200022
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64695
专题北京大学公共卫生学院_毒理学系
作者单位1.Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
2.Peking Univ, Dept Toxicol, Hlth Sci Ctr, Beijing 100083, Peoples R China
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Yi, ZC,Zhang, M,Fu, JL,et al. Down-regulation of mitotic checkpoint in transformed human embryo lung fibroblasts induced by N-methyl-N-′-nitro-N-nitrosoguaridine[J]. CHINESE MEDICAL JOURNAL,2004,117(7):1079-1083.
APA Yi, ZC,Zhang, M,Fu, JL,Wang, Z,&Zhou, ZC.(2004).Down-regulation of mitotic checkpoint in transformed human embryo lung fibroblasts induced by N-methyl-N-′-nitro-N-nitrosoguaridine.CHINESE MEDICAL JOURNAL,117(7),1079-1083.
MLA Yi, ZC,et al."Down-regulation of mitotic checkpoint in transformed human embryo lung fibroblasts induced by N-methyl-N-′-nitro-N-nitrosoguaridine".CHINESE MEDICAL JOURNAL 117.7(2004):1079-1083.
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