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学科主题: 基础医学
题名:
Triptolide protects dopaminergic neurons from inflammation-mediated damage lipopolysaccharide intranigral induced by injection
作者: Zhou, HF; Liu, XY; Niu, DB; Li, FQ; He, QH; Wang, XM
关键词: triptolide ; Tripterygium wilfordii Hook F ; inflammation ; microglia ; Parkinson&prime ; s disease ; neurodegeneration ; proinflammatory cytokines ; neuroinflammation
刊名: NEUROBIOLOGY OF DISEASE
发表日期: 2005-04-01
卷: 18, 期:3, 页:441-449
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: WILFORDII HOOK F ; PARKINSONS-DISEASE ; TRIPTERYGIUM-WILFORDII ; TNF-ALPHA ; IN-VITRO ; MICROGLIA ; SYSTEM ; BRAIN ; MICE ; 6-HYDROXYDOPAMINE
英文摘要:

Converging lines of evidence suggest that neuroinflammatory processes may account for the progressive death of dopaminergic neurons in Parkinson′s disease (PD). Therefore, anti-inflammatory strategies have attracted much interest for their potential to prevent further deterioration of PD. Our previous study showed that triptolide, a traditional Chinese herbal compound with anti-inflammatory and immunosuppressive properties, protected dopaminergic neurons from lipopolysaccharide (LPS)-induced damage in primary embryonic midbrain cell cultures. To examine further if triptolide can protect dopaminergic neurons from inflammation-mediated damage in vivo, microglial activation and injury of dopaminergic neurons were induced by LPS intranigral injection, and the effects of triptolide treatment on microglial activation and survival ratio and function of dopaminergic neurons were investigated. Our results demonstrated that microglial activation induced by a single intranigral dose of 10 mu g of LPS reduced the survival ratio of tyrosine hydroxylase-immunoreactive (TH-ir) neurons in the substantia nigra pars compacta (SNpc) to 29% and the content of dopamine (DA) in striatum to 37%, of the non-injected side. Intriguingly, treatment with triptolide of 5 mu g/kg for 24 days once per day dramatically improved the survival rate of TH-ir neurons in the SNpc to 79% of the non-injected side. Meanwhile, treatment with triptolide of 1 or 5 mu g/kg for 24 days once per day significantly improved DA level in striatum to 70% and 68% of the non-injected side, respectively. Complement receptor 3 (CR3) immunohistochemical staining revealed that triptolide treatment potently inhibited LPS-elicited deleterious activation of microglia in SNpc. The excessive production of cytokines, such as tumor necrosis factor (TNF)-alpha and interleukin (IL)-1 beta, was significantly abolished by triptolide administration. These results, together with our previous data in vitro, highly suggest the effectiveness of triptolide in protecting dopaminergic neurons against inflammatory challenge. (c) 2004 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000227820500003
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64700
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: 1.Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
2.Capital Univ Med Sci, Beijing Inst Neurosci, Beijing 100054, Peoples R China

Recommended Citation:
Zhou, HF,Liu, XY,Niu, DB,et al. Triptolide protects dopaminergic neurons from inflammation-mediated damage lipopolysaccharide intranigral induced by injection[J]. NEUROBIOLOGY OF DISEASE,2005,18(3):441-449.
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