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学科主题: 临床医学
题名:
Binding capacity of in vitro deglycosylated IgA1 to human mesangial cells
作者: Zhang, JJ; Xu, LX; Zhang, Y; Zhao, MH
关键词: binding ; IgA1 ; DesIgA1 ; DesIgA1 /DeGalIgA1 ; IgAN ; mesangial cell ; Neu5Ac ; gat ; GalNAc ; deglycosylation ; radioligand
刊名: CLINICAL IMMUNOLOGY
发表日期: 2006-04-01
DOI: 10.1016/j.clim.2005.12.002
卷: 119, 期:1, 页:103-109
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: ABERRANTLY GLYCOSYLATED IGA ; O-LINKED OLIGOSACCHARIDES ; HUMAN SERUM IGA1 ; IMMUNE-COMPLEXES ; NEPHROPATHY ; RECEPTOR ; MODULATION ; CHAIN ; RAT
英文摘要:

IgA nephropathy (IgAN) is the most common glomerular disease and it is characterized by deposition of IgA1 molecules in mesangium. Recent studies had demonstrated that serum and mesangial IgA1 in IgAN were deglycosylated and IgA1 could bind to human mesangial cells (HMC) through a novel receptor. The aim of the current study is to investigate and compare the binding capacities of different in vitro deglycosylated IgA1 on human mesangial cells. Serum IgA1 was purified by jacalin affinity chromatography and then was desialylated (DesIgA1) and/or degalactosylated (Des/DeGalIgA1) with neuraminidase and/or 1 galactosidase. The efficacy of deglycosylations was assessed by Peanut agglutinin (PNA) and Vicia villosa (VV) lectin. The sizes of normal IgA1 and deglycosylated IgA1 were determined by Sephacryl S-300 chromatography and binding capacities to primary HMC were evaluated by radioligand binding assays. Normal IgA1 and deglycosylated IgA1 could bind to HMC in a dose-dependent, saturable manner. The maximal. binding capacities and binding sites/cell of DesIgA1 and Des/DeGalIgA were significantly higher than that of normal IgA1. However, more aggregated IgA1 was found in DesIgA1 and Des/DeGalIgA1. Scatchard analysis revealed a similar K-d of normal IgA1 and deglycosylated IgA1. The current study suggested that the binding capacities of DesIgA1 and Des/DeGalIgA1 to HMC were significantly higher than that of normal IgA1, which at least in part was due to more macromolecular IgA1 in deglycoslated IgA1. However, there were no significant differences in the affinities of normal IgA1, DesIgA1 and Des/DeGalIgA1 with HMC. Deglycosylated IgA1 might play an important role in pathogenesis of IgAN. (c) 2005 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000236138700013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64703
Appears in Collections:北京大学第一临床医学院_肾脏内科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Div Renal, Beijing 100034, Peoples R China
2.Peking Univ, Hosp 1, Inst Nephrol, Beijing 100034, Peoples R China

Recommended Citation:
Zhang, JJ,Xu, LX,Zhang, Y,et al. Binding capacity of in vitro deglycosylated IgA1 to human mesangial cells[J]. CLINICAL IMMUNOLOGY,2006,119(1):103-109.
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