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Binding capacity of in vitro deglycosylated IgA1 to human mesangial cells
Zhang, JJ; Xu, LX; Zhang, Y; Zhao, MH
关键词binding IgA1 DesIgA1 DesIgA1 /DeGalIgA1 IgAN mesangial cell Neu5Ac gat GalNAc deglycosylation radioligand
刊名CLINICAL IMMUNOLOGY
2006-04-01
DOI10.1016/j.clim.2005.12.002
119期:1页:103-109
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology
研究领域[WOS]Immunology
关键词[WOS]ABERRANTLY GLYCOSYLATED IGA ; O-LINKED OLIGOSACCHARIDES ; HUMAN SERUM IGA1 ; IMMUNE-COMPLEXES ; NEPHROPATHY ; RECEPTOR ; MODULATION ; CHAIN ; RAT
英文摘要

IgA nephropathy (IgAN) is the most common glomerular disease and it is characterized by deposition of IgA1 molecules in mesangium. Recent studies had demonstrated that serum and mesangial IgA1 in IgAN were deglycosylated and IgA1 could bind to human mesangial cells (HMC) through a novel receptor. The aim of the current study is to investigate and compare the binding capacities of different in vitro deglycosylated IgA1 on human mesangial cells. Serum IgA1 was purified by jacalin affinity chromatography and then was desialylated (DesIgA1) and/or degalactosylated (Des/DeGalIgA1) with neuraminidase and/or 1 galactosidase. The efficacy of deglycosylations was assessed by Peanut agglutinin (PNA) and Vicia villosa (VV) lectin. The sizes of normal IgA1 and deglycosylated IgA1 were determined by Sephacryl S-300 chromatography and binding capacities to primary HMC were evaluated by radioligand binding assays. Normal IgA1 and deglycosylated IgA1 could bind to HMC in a dose-dependent, saturable manner. The maximal. binding capacities and binding sites/cell of DesIgA1 and Des/DeGalIgA were significantly higher than that of normal IgA1. However, more aggregated IgA1 was found in DesIgA1 and Des/DeGalIgA1. Scatchard analysis revealed a similar K-d of normal IgA1 and deglycosylated IgA1. The current study suggested that the binding capacities of DesIgA1 and Des/DeGalIgA1 to HMC were significantly higher than that of normal IgA1, which at least in part was due to more macromolecular IgA1 in deglycoslated IgA1. However, there were no significant differences in the affinities of normal IgA1, DesIgA1 and Des/DeGalIgA1 with HMC. Deglycosylated IgA1 might play an important role in pathogenesis of IgAN. (c) 2005 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000236138700013
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被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64703
专题北京大学第一临床医学院_肾脏内科
作者单位1.Peking Univ, Hosp 1, Div Renal, Beijing 100034, Peoples R China
2.Peking Univ, Hosp 1, Inst Nephrol, Beijing 100034, Peoples R China
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GB/T 7714
Zhang, JJ,Xu, LX,Zhang, Y,et al. Binding capacity of in vitro deglycosylated IgA1 to human mesangial cells[J]. CLINICAL IMMUNOLOGY,2006,119(1):103-109.
APA Zhang, JJ,Xu, LX,Zhang, Y,&Zhao, MH.(2006).Binding capacity of in vitro deglycosylated IgA1 to human mesangial cells.CLINICAL IMMUNOLOGY,119(1),103-109.
MLA Zhang, JJ,et al."Binding capacity of in vitro deglycosylated IgA1 to human mesangial cells".CLINICAL IMMUNOLOGY 119.1(2006):103-109.
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