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Role of protein tyrosine kinase in IL-1 beta induced activation of mitogen-activated protein kinase in fibroblast-like synoviocytes of rheumatoid arthritis
Lu, HS; Sun, TH; Yao, LB; Zhang, YJ
关键词arthritis fibroblasts IL-1beta mitogen-activated protein kinase protein tyrosine kinase genistein
刊名CHINESE MEDICAL JOURNAL
2000-10-01
113期:10页:872-876
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]GROWTH-FACTOR ; INTERLEUKIN-1 ; PROLIFERATION
英文摘要

Objectives To study mitogen-activated protein kinase (MAPKs) activation in fibroblast-like synoviocytes (FLS) of rheumatoid arthritis (RA) under the stimulation of IL-1 beta, and to elucidate the role of protein tyrosine kinase (PTK) in the activation of MAPKs.

Methods Primary cultures of RA FLS were used. Western blot was applied to examine transient changes in protein tyrosine phosphorylation status and MAPKs activation in RA FLS stimulated with IL-1 beta at various doses, and over different periods. Genistein, the specific PTK inhibitor, was used to evaluate the inhibitory role in activation of MAPKs by IL-1 beta.

Results IL-1 beta transiently increased protein tyrosine phosphorylation, and activated the MAPKs cascades (mainly ERK2, JNK(2) and P-38) in RA FLS. There was no obvious difference in MAPKs activation among different doses of IL-1 beta (1 IU/ml, 10 IU/ml, 100 IU/ml), but the peak activation of ERK2, JNK(2) and P-38 took place at 5 min, 15 min and 1 min, respectively, after stimulation with IL-1 beta. The activation of ERK2 was inhibited by genistein, but the inhibitory role on that of JNK and P-38 was relatively weak.

Conclusions During signal transduction of IL-1 beta in RA FLS, tyrosine phosphorylation was increased transiently, the MAPKs cascade was activated in a few minutes, and there was heterogenicity in the activation among three subfamily members. PTK had a role in the activation of ERK, but had weak effects on that of JNK and P-38.

语种英语
WOS记录号WOS:000089840800002
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被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64709
专题北京大学第二临床医学院
作者单位1.Beijing Med Univ, Peoples Hosp, Arthritis Clin, Beijing 100044, Peoples R China
2.Beijing Med Univ, Peoples Hosp, Res Ctr, Beijing 100044, Peoples R China
3.Beijing Med Univ, Peoples Hosp, Dept Gynecol, Beijing 100044, Peoples R China
4.Fourth Mil Med Univ, Dept Biochem, Xian 710032, Peoples R China
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GB/T 7714
Lu, HS,Sun, TH,Yao, LB,et al. Role of protein tyrosine kinase in IL-1 beta induced activation of mitogen-activated protein kinase in fibroblast-like synoviocytes of rheumatoid arthritis[J]. CHINESE MEDICAL JOURNAL,2000,113(10):872-876.
APA Lu, HS,Sun, TH,Yao, LB,&Zhang, YJ.(2000).Role of protein tyrosine kinase in IL-1 beta induced activation of mitogen-activated protein kinase in fibroblast-like synoviocytes of rheumatoid arthritis.CHINESE MEDICAL JOURNAL,113(10),872-876.
MLA Lu, HS,et al."Role of protein tyrosine kinase in IL-1 beta induced activation of mitogen-activated protein kinase in fibroblast-like synoviocytes of rheumatoid arthritis".CHINESE MEDICAL JOURNAL 113.10(2000):872-876.
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