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学科主题基础医学
1A6/DRIM, the human UTP20 functions in 28S and 5.8S rRNA processing
Kong RuiRui2,3; Han Wei3; Weidle, Ulrich H.4; Ning Tao2; Du XiaoJuan1,3; Ke Yang2,3
关键词1A6/DRIM UTP20 small-subunit (SSU) processome 28S and 5.8S rRNA processing U8 small nucleolar RNA (U8 snoRNA)
刊名CHINESE SCIENCE BULLETIN
2010-06-01
DOI10.1007/s11434-010-3166-8
55期:17页:1770-1776
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]SMALL NUCLEOLAR RNA ; SMALL-SUBUNIT PROCESSOME ; CELL-CYCLE PROGRESSION ; XENOPUS-OOCYTES ; PEBOW-COMPLEX ; U3 SNORNP ; PROTEIN ; BIOGENESIS ; MATURATION ; BOP1
英文摘要

1A6/DRIM has been identified as UTP20, a small subunit processome component, functioning in 18S rRNA processing. In the present study, the maturation of 28S rRNA and 5.8S rRNA was inhibited when 1A6/DRIM was silenced in HeLa cells; and co-incidently, an accumulation of 32S rRNA precursor was observed. Immunoprecipitation was performed with the anti-1A6/DRIM antibody, followed by Northern blot with the ITS2 probe. The results showed that 1A6/DRIM was associated with both 32S and 12S rRNA precursors in vivo. The expression profile of 1A6/DRIM during rRNA processing was investigated by sucrose density gradient fractionation in combination with Western blot analysis. The results demonstrated that 1A6/DRIM was involved in the pre-60S particles in addition to the pre-40S particles and co-sediment with the 32S and 12S rRNA precursors in the nucleolus. Furthermore, the interaction of U8 snoRNA with 1A6/DRIM was revealed by immunoprecipitation. These results demonstrated that 1A6/DRIM interacted with both 32S rRNA and U8 snoRNA, being involved in 28S rRNA and 5.8S rRNA processing.

语种英语
WOS记录号WOS:000278900800012
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64724
专题北京大学基础医学院_细胞生物学系
北京大学基础医学院
医学人文研究院/公共教学部_哲学与社会科学系
北京大学临床肿瘤学院_遗传学研究室
作者单位1.Roche Diagnost GmbH, Div Pharma, D-82372 Penzberg, Germany
2.Peking Univ, Hlth Sci Ctr, Dept Cell Biol, Beijing 100191, Peoples R China
3.Peking Univ, Sch Oncol, Key Lab Carcinogenesis & Translat Res,Minist Educ, Genet Lab,Beijing Canc Hosp & Inst, Beijing 100142, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Canc Res Ctr, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Kong RuiRui,Han Wei,Weidle, Ulrich H.,et al. 1A6/DRIM, the human UTP20 functions in 28S and 5.8S rRNA processing[J]. CHINESE SCIENCE BULLETIN,2010,55(17):1770-1776.
APA Kong RuiRui,Han Wei,Weidle, Ulrich H.,Ning Tao,Du XiaoJuan,&Ke Yang.(2010).1A6/DRIM, the human UTP20 functions in 28S and 5.8S rRNA processing.CHINESE SCIENCE BULLETIN,55(17),1770-1776.
MLA Kong RuiRui,et al."1A6/DRIM, the human UTP20 functions in 28S and 5.8S rRNA processing".CHINESE SCIENCE BULLETIN 55.17(2010):1770-1776.
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