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学科主题: 基础医学
题名:
1A6/DRIM, the human UTP20 functions in 28S and 5.8S rRNA processing
作者: Kong RuiRui2,3; Han Wei3; Weidle, Ulrich H.4; Ning Tao2; Du XiaoJuan1,3; Ke Yang2,3
关键词: 1A6/DRIM ; UTP20 ; small-subunit (SSU) processome ; 28S and 5.8S rRNA processing ; U8 small nucleolar RNA (U8 snoRNA)
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2010-06-01
DOI: 10.1007/s11434-010-3166-8
卷: 55, 期:17, 页:1770-1776
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: SMALL NUCLEOLAR RNA ; SMALL-SUBUNIT PROCESSOME ; CELL-CYCLE PROGRESSION ; XENOPUS-OOCYTES ; PEBOW-COMPLEX ; U3 SNORNP ; PROTEIN ; BIOGENESIS ; MATURATION ; BOP1
英文摘要:

1A6/DRIM has been identified as UTP20, a small subunit processome component, functioning in 18S rRNA processing. In the present study, the maturation of 28S rRNA and 5.8S rRNA was inhibited when 1A6/DRIM was silenced in HeLa cells; and co-incidently, an accumulation of 32S rRNA precursor was observed. Immunoprecipitation was performed with the anti-1A6/DRIM antibody, followed by Northern blot with the ITS2 probe. The results showed that 1A6/DRIM was associated with both 32S and 12S rRNA precursors in vivo. The expression profile of 1A6/DRIM during rRNA processing was investigated by sucrose density gradient fractionation in combination with Western blot analysis. The results demonstrated that 1A6/DRIM was involved in the pre-60S particles in addition to the pre-40S particles and co-sediment with the 32S and 12S rRNA precursors in the nucleolus. Furthermore, the interaction of U8 snoRNA with 1A6/DRIM was revealed by immunoprecipitation. These results demonstrated that 1A6/DRIM interacted with both 32S rRNA and U8 snoRNA, being involved in 28S rRNA and 5.8S rRNA processing.

语种: 英语
所属项目编号: 2006AA02A402 ; 2008AA02Z131 ; 30771224 ; 985-2-016-24 ; 2006CB943603
项目资助者: National High-Tech Research and Development Program of China ; National Natural Science Foundation of China ; Ministry of Education of China ; National Key Basic Research Program of China
WOS记录号: WOS:000278900800012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64724
Appears in Collections:基础医学院_细胞生物学系_期刊论文

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作者单位: 1.Roche Diagnost GmbH, Div Pharma, D-82372 Penzberg, Germany
2.Peking Univ, Hlth Sci Ctr, Dept Cell Biol, Beijing 100191, Peoples R China
3.Peking Univ, Sch Oncol, Key Lab Carcinogenesis & Translat Res,Minist Educ, Genet Lab,Beijing Canc Hosp & Inst, Beijing 100142, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Canc Res Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Kong RuiRui,Han Wei,Weidle, Ulrich H.,et al. 1A6/DRIM, the human UTP20 functions in 28S and 5.8S rRNA processing[J]. CHINESE SCIENCE BULLETIN,2010,55(17):1770-1776.
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