IR@PKUHSC  > 北京大学第三临床医学院
学科主题临床医学
MicroRNA-376c Impairs Transforming Growth Factor-beta and Nodal Signaling to Promote Trophoblast Cell Proliferation and Invasion
Fu, Guodong1; Ye, Gang1; Nadeem, Lubna1; Ji, Lei2; Manchanda, Tanita1; Wang, Yongqing3; Zhao, Yangyu3; Qiao, Jie3; Wang, Yan-Ling2; Lye, Stephen4; Yang, Burton B.5; Peng, Chun1
关键词ALK5 ALK7 miR-376c Nodal placenta preeclampsia TGF-beta
刊名HYPERTENSION
2013-04-01
DOI10.1161/HYPERTENSIONAHA.111.203489
61期:4页:864-+
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Peripheral Vascular Disease
资助者Canadian Institute of Health Research ; National Natural Sciences Foundation from China ; Ontario Graduate Scholarship ; Ontario Women&prime ; s Health Council/CIHR ; Canadian Institute of Health Research ; National Natural Sciences Foundation from China ; Ontario Graduate Scholarship ; Ontario Women&prime ; s Health Council/CIHR
研究领域[WOS]Cardiovascular System & Cardiology
关键词[WOS]PREECLAMPSIA ; EXPRESSION ; MIGRATION ; PLACENTA ; RECEPTOR ; PLASMA ; CANCER ; DIFFERENTIATION ; TRANSCRIPTION ; ANGIOGENESIS
英文摘要

Preeclampsia is a major disorder of pregnancy and a leading cause of maternal and perinatal morbidity and mortality. MicroRNAs are small noncoding RNAs that regulate gene expression posttranscriptionally. In this study, we examined the expression of miR-376c and found that miR-376c levels were downregulated in both placental and plasma samples collected from preeclamptic patients, when compared with the normal pregnant women at the same gestational stage. Overexpression of miR-376c induced trophoblast cell proliferation, migration, and invasion in HTR8/SVneo cells and promoted placental explant outgrowth. In contrast, inhibition of endogenous miR-376c resulted in a decrease in trophoblast cell invasion and placental explant outgrowth. We identified activin receptor-like kinase 5 (ALK5), a type I receptor for transforming growth factor-beta, and ALK7, a type I receptor for Nodal, as targets of miR-376c. Overexpression of miR-376c repressed transforming growth factor-beta and Nodal functions, whereas overexpression of ALK5 and ALK7 reversed the effects of miR-376c. These results demonstrate that miR-376c inhibits both ALK5 and ALK7 expression to impair transforming growth factor-beta/Nodal signaling, leading to increases in cell proliferation and invasion. An unbalanced Nodal/transforming growth factor-beta and miR-376c expression may lead to the development of preeclampsia. (Hypertension. 2013;61:864-872.) circle Online Data Supplement

语种英语
所属项目编号CIHR MOP-81370 ; CCI-92222 ; 81025004 ; 3081120430
资助者Canadian Institute of Health Research ; National Natural Sciences Foundation from China ; Ontario Graduate Scholarship ; Ontario Women&prime ; s Health Council/CIHR ; Canadian Institute of Health Research ; National Natural Sciences Foundation from China ; Ontario Graduate Scholarship ; Ontario Women&prime ; s Health Council/CIHR
WOS记录号WOS:000316112800031
Citation statistics
Cited Times:58[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64725
Collection北京大学第三临床医学院
作者单位1.York Univ, Dept Biol, Toronto, ON M3J 2R7, Canada
2.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China
3.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Beijing 100871, Peoples R China
4.Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Res Ctr Womens & Infants Hlth, Toronto, ON M5G 1X5, Canada
5.Sunnybrook Hlth Sci Ctr, Sunnybrook Res Inst, Toronto, ON M4N 3M5, Canada
Recommended Citation
GB/T 7714
Fu, Guodong,Ye, Gang,Nadeem, Lubna,et al. MicroRNA-376c Impairs Transforming Growth Factor-beta and Nodal Signaling to Promote Trophoblast Cell Proliferation and Invasion[J]. HYPERTENSION,2013,61(4):864-+.
APA Fu, Guodong.,Ye, Gang.,Nadeem, Lubna.,Ji, Lei.,Manchanda, Tanita.,...&Peng, Chun.(2013).MicroRNA-376c Impairs Transforming Growth Factor-beta and Nodal Signaling to Promote Trophoblast Cell Proliferation and Invasion.HYPERTENSION,61(4),864-+.
MLA Fu, Guodong,et al."MicroRNA-376c Impairs Transforming Growth Factor-beta and Nodal Signaling to Promote Trophoblast Cell Proliferation and Invasion".HYPERTENSION 61.4(2013):864-+.
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