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学科主题: 基础医学
题名:
Adenovirus vector-mediated FAM176A overexpression induces cell death in human H1299 non-small cell lung cancer cells
作者: Xie, Hong1; Hu, Jia1,2; Pan, Huan2; Lou, Yaxin2,3; Lv, Ping1,2; Chen, Yingyu1,2
关键词: Anti-tumor activity ; Apoptosis ; Autophagy ; Cell cycle ; FAM176A
刊名: BMB REPORTS
发表日期: 2014-02-28
DOI: 10.5483/BMBRep.2014.47.2.090
卷: 47, 期:2, 页:104-109
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: TRANSMEMBRANE PROTEIN ; EXPRESSION ; P53 ; APOPTOSIS ; AUTOPHAGY ; TMEM166 ; CARCINOMA ; CHINA
英文摘要:

FAM176A (family with sequence similarity 176 member A) is a novel molecule related to programmed cell death. A decreased expression of FAM176A has been found in several types of human tumors in including lung cancers. In the present study, we investigated the biological activities of FAM176A on the human non-small cell lung cancer cell line H1299 cells. We constructed a recombinant adenovirus 5-FAM176A vector (Ad5-FAM176A) and evaluated the expression and anti-tumor activities in vitro. Cell viability analysis revealed that the adenovirus-mediated increase of FAM176A inhibited the growth of the tumor cells in a dose-and time-dependent manner. This inhibitory effect was mediated by both autophagy and apoptosis that involved caspase activation. In addition, cell cycle analysis suggested that Ad5-FAM176A could induce cell cycle arrest at the G2/M phase, all of which suggested that adenovirus-mediated FAM176A gene transfer might present a new therapeutic approach for lung cancer treatment.

语种: 英语
所属项目编号: 2011CB910103 ; 30771057 ; J1030831/J0108
项目资助者: National Key Project for Basic Research of China (973) ; National Natural Science Foundation of China ; National Science Foundation for Fostering Talents in Basic Research of the National
WOS记录号: WOS:000332148200008
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64727
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Minist Hlth, Key Lab Med Immunol, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Ctr Human Dis Genom, Beijing 100191, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Med & Hlth Analyt Ctr, Prote Lab, Beijing 100191, Peoples R China

Recommended Citation:
Xie, Hong,Hu, Jia,Pan, Huan,et al. Adenovirus vector-mediated FAM176A overexpression induces cell death in human H1299 non-small cell lung cancer cells[J]. BMB REPORTS,2014,47(2):104-109.
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