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Determination of Zygosity in Adult Chinese Twins Using the 450K Methylation Array versus Questionnaire Data
Wang, Biqi1; Gao, Wenjing1; Yu, Canqing1; Cao, Weihua1; Lv, Jun1; Wang, Shengfeng1; Pang, Zengchang2; Cong, Liming3; Wang, Hua4; Wu, Xianping5; Li, Liming1
刊名PLOS ONE
2015-04-30
DOI10.1371/journal.pone.0123992
10期:4
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者National Natural Science Foundation of China ; Specific Research Project of Health Public Service, Ministry of Health, China ; National Natural Science Foundation of China ; Specific Research Project of Health Public Service, Ministry of Health, China
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]GENOME-WIDE ASSOCIATION ; DNA METHYLATION ; REGISTRY ; DIAGNOSIS ; UPDATE ; BIRTH ; YOUNG ; PAIRS ; AGE
英文摘要

Previous studies have shown that both single nucleotide polymorphisms (SNPs) and questionnaires-based method can be used for twin zygosity determination, but few validation studies have been conducted using Chinese populations. In the current study, we recruited 192 same sex Chinese adult twin pairs to evaluate the validity of using genetic markers-based method and questionnaire-based method in zygosity determination. We considered the relatedness analysis based on more than 0.6 million SNPs genotyping as the golden standards for zygosity determination. After quality control, qualified twins were left for relatedness analysis based on identical by descent calculation. Then those same sex twin pairs were included in the zygosity questionnaire validation analysis. Logistic regression model was applied to assess the discriminant ability of age, sex and the three questions in zygosity determination. Leave one out cross-validation was used as a measurement of internal validation. The results of zygosity determination based on 65 SNPs in 450k methylation array were all consistent with genotyping. Age, gender, questions of appearance confused by strangers and previously perceived zygosity consisted of the most predictable model with a consistency rate of 0.8698, cross validation predictive error of 0.1347. For twin studies with genotyping and\or 450k methylation array, there would be no need to conduct other zygosity testing for the sake of costs consideration.

语种英语
所属项目编号81202264 ; 201002007
资助者National Natural Science Foundation of China ; Specific Research Project of Health Public Service, Ministry of Health, China ; National Natural Science Foundation of China ; Specific Research Project of Health Public Service, Ministry of Health, China
WOS记录号WOS:000353713100026
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64764
专题北京大学公共卫生学院
作者单位1.Peking Univ, Sch Publ Hlth, Dept Epidemiol & Biostat, Beijing 100191, Peoples R China
2.Qingdao Ctr Dis Control & Prevent, Qingdao 266033, Peoples R China
3.Zhejiang Ctr Dis Control & Prevent, Hangzhou 310051, Zhejiang, Peoples R China
4.Jiangsu Ctr Dis Control & Prevent, Nanjing 210009, Jiangsu, Peoples R China
5.Sichuan Ctr Dis Control & Prevent, Chengdu 610041, Peoples R China
推荐引用方式
GB/T 7714
Wang, Biqi,Gao, Wenjing,Yu, Canqing,et al. Determination of Zygosity in Adult Chinese Twins Using the 450K Methylation Array versus Questionnaire Data[J]. PLOS ONE,2015,10(4).
APA Wang, Biqi.,Gao, Wenjing.,Yu, Canqing.,Cao, Weihua.,Lv, Jun.,...&Li, Liming.(2015).Determination of Zygosity in Adult Chinese Twins Using the 450K Methylation Array versus Questionnaire Data.PLOS ONE,10(4).
MLA Wang, Biqi,et al."Determination of Zygosity in Adult Chinese Twins Using the 450K Methylation Array versus Questionnaire Data".PLOS ONE 10.4(2015).
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