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Effect of Robo1 on Retinal Pigment Epithelial Cells and Experimental Proliferative Vitreoretinopathy
Huang, Lvzhen1; Xu, Yongsheng1; Yu, Wenzhen1; Li, Yingjie3; Chu, Liqun1; Dong, Jianqiang2; Li, Xiaoxin1
刊名INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
2010-06-01
DOI10.1167/iovs.09-3779
51期:6页:3193-3204
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
资助者National Basic Research Program of China 973 Program ; Research Fund for the Doctoral Program of Higher Education of China Program ; People&prime ; s Hospital Research and Development Fund ; National Basic Research Program of China 973 Program ; Research Fund for the Doctoral Program of Higher Education of China Program ; People&prime ; s Hospital Research and Development Fund
研究领域[WOS]Ophthalmology
关键词[WOS]MASSIVE PERIRETINAL PROLIFERATION ; EPIRETINAL MEMBRANES ; AXON GUIDANCE ; CELLULAR PROLIFERATION ; DIABETIC-RETINOPATHY ; TUMOR ANGIOGENESIS ; EXPRESSION ; SLIT ; GROWTH ; INHIBITION
英文摘要

PURPOSE. The Roundabout (Robo) family of proteins is related to the transmembrane receptors and plays a major role in neurogenesis. However, the role of the Robo proteins in proliferative retinopathy has not yet been defined. This study was conducted to determine whether Robo1 is expressed in the retina of patients with proliferative retinal disease and whether it has a pathobiological role in the disease.

METHODS. Immunohistochemistry was used to determine the presence and distribution of Robo1 in the pathologic membranes in proliferative retinopathy. Small interfering (si) RNA technology was used to knockdown Robo1 expression and to study its effects on retinal pigment epithelial (RPE) cells in vitro. The impact on PVR development of blocking Robo1 expression was determined by applying specific siRNA in a PVR rabbit model. The prevalences of PVR and retinal detachment were determined by indirect ophthalmoscope on days 1, 3, 7, 14, 21, and 28 after the injection of RPE cells into the vitreous.

RESULTS. Immunohistochemistry showed that Robo1 expression was detected in GFAP-labeled glial cells and cytokeratin-labeled RPE cells in proliferative membranes. Robo1 expression was also detected in CD31-labeled vascular endothelial cells. Knockdown of Robo1 expression not only reduced human RPE cell proliferation in vitro but also effectively suppressed the development of PVR in a rabbit model.

CONCLUSIONS. Robo1 is present in the extracellular matrix of proliferative membranes and may be derived from dedifferentiated RPE cells. Silencing the expression of Robo1 in RPE cells inhibited cell proliferation and suppressed the development of PVR in an animal model, indicating a potential therapeutic usefulness in treating PVR. (Invest Ophthalmol Vis Sci. 2010;51:3193-3204) DOI:10.1167/iovs.09-3779

语种英语
所属项目编号2005CB724307 ; 20090001110083 ; RDB2009-43
资助者National Basic Research Program of China 973 Program ; Research Fund for the Doctoral Program of Higher Education of China Program ; People&prime ; s Hospital Research and Development Fund ; National Basic Research Program of China 973 Program ; Research Fund for the Doctoral Program of Higher Education of China Program ; People&prime ; s Hospital Research and Development Fund
WOS记录号WOS:000277846500050
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64768
专题北京大学第二临床医学院_眼科
作者单位1.Peking Univ Peoples Hosp, Dept Ophthalmol, Beijing 100044, Peoples R China
2.Peking Univ Peoples Hosp, Ctr Lab, Beijing 100044, Peoples R China
3.Peking Univ Beijing Canc Hosp Inst, Dept Hepatobiliary Surg, Beijing, Peoples R China
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GB/T 7714
Huang, Lvzhen,Xu, Yongsheng,Yu, Wenzhen,et al. Effect of Robo1 on Retinal Pigment Epithelial Cells and Experimental Proliferative Vitreoretinopathy[J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,2010,51(6):3193-3204.
APA Huang, Lvzhen.,Xu, Yongsheng.,Yu, Wenzhen.,Li, Yingjie.,Chu, Liqun.,...&Li, Xiaoxin.(2010).Effect of Robo1 on Retinal Pigment Epithelial Cells and Experimental Proliferative Vitreoretinopathy.INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,51(6),3193-3204.
MLA Huang, Lvzhen,et al."Effect of Robo1 on Retinal Pigment Epithelial Cells and Experimental Proliferative Vitreoretinopathy".INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 51.6(2010):3193-3204.
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