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学科主题: 基础医学
题名:
A Small Heat Shock Protein Enables Escherichia coli To Grow at a Lethal Temperature of 50 degrees C Conceivably by Maintaining Cell Envelope Integrity
作者: Ezemaduka, Anastasia N.1,2; Yu, Jiayu1,2; Shi, Xiaodong1,2,4; Zhang, Kaiming3; Yin, Chang-Cheng3; Fu, Xinmiao1,2; Chang, Zengyi1,2
刊名: JOURNAL OF BACTERIOLOGY
发表日期: 2014-06-01
DOI: 10.1128/JB.01473-14
卷: 196, 期:11, 页:2004-2011
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
关键词[WOS]: CHAPERONE-LIKE ACTIVITY ; MYCOBACTERIUM-TUBERCULOSIS ; CAENORHABDITIS-ELEGANS ; ENHANCES THERMOTOLERANCE ; ELEVATED-TEMPERATURES ; MOLECULAR CHAPERONES ; PLASMA-MEMBRANE ; GENE ; EVOLUTION ; HSP16.3
英文摘要:

It is essential for organisms to adapt to fluctuating growth temperatures. Escherichia coli, a model bacterium commonly used in research and industry, has been reported to grow at a temperature lower than 46.5 degrees C. Here we report that the heterologous expression of the 17-kDa small heat shock protein from the nematode Caenorhabditis elegans, CeHSP17, enables E. coli cells to grow at 50 degrees C, which is their highest growth temperature ever reported. Strikingly, CeHSP17 also rescues the thermal lethality of an E. coli mutant deficient in degP, which encodes a protein quality control factor localized in the periplasmic space. Mechanistically, we show that CeHSP17 is partially localized in the periplasmic space and associated with the inner membrane of E. coli, and it helps to maintain the cell envelope integrity of the E. coli cells at the lethal temperatures. Together, our data indicate that maintaining the cell envelope integrity is crucial for the E. coli cells to grow at high temperatures and also shed new light on the development of thermophilic bacteria for industrial application.

语种: 英语
所属项目编号: 2012CB917300 ; 31170738 ; 31100559 ; 31270804
项目资助者: National Basic Research Program of China (973 Program) ; National Natural Science Foundation of China
WOS记录号: WOS:000335909500009
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64772
Appears in Collections:基础医学院_生物物理学系_期刊论文

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作者单位: 1.Peking Univ, Ctr Prot Sci, Beijing 100871, Peoples R China
2.Peking Univ, Sch Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Biophys, Beijing 100871, Peoples R China
4.Xuzhou Med Coll, Jiangsu Prov Key Lab Anesthesiol, Xuzhou, Peoples R China

Recommended Citation:
Ezemaduka, Anastasia N.,Yu, Jiayu,Shi, Xiaodong,et al. A Small Heat Shock Protein Enables Escherichia coli To Grow at a Lethal Temperature of 50 degrees C Conceivably by Maintaining Cell Envelope Integrity[J]. JOURNAL OF BACTERIOLOGY,2014,196(11):2004-2011.
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