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A Small Heat Shock Protein Enables Escherichia coli To Grow at a Lethal Temperature of 50 degrees C Conceivably by Maintaining Cell Envelope Integrity
Ezemaduka, Anastasia N.1,2; Yu, Jiayu1,2; Shi, Xiaodong1,2,4; Zhang, Kaiming3; Yin, Chang-Cheng3; Fu, Xinmiao1,2; Chang, Zengyi1,2
刊名JOURNAL OF BACTERIOLOGY
2014-06-01
DOI10.1128/JB.01473-14
196期:11页:2004-2011
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Microbiology
研究领域[WOS]Microbiology
关键词[WOS]CHAPERONE-LIKE ACTIVITY ; MYCOBACTERIUM-TUBERCULOSIS ; CAENORHABDITIS-ELEGANS ; ENHANCES THERMOTOLERANCE ; ELEVATED-TEMPERATURES ; MOLECULAR CHAPERONES ; PLASMA-MEMBRANE ; GENE ; EVOLUTION ; HSP16.3
英文摘要

It is essential for organisms to adapt to fluctuating growth temperatures. Escherichia coli, a model bacterium commonly used in research and industry, has been reported to grow at a temperature lower than 46.5 degrees C. Here we report that the heterologous expression of the 17-kDa small heat shock protein from the nematode Caenorhabditis elegans, CeHSP17, enables E. coli cells to grow at 50 degrees C, which is their highest growth temperature ever reported. Strikingly, CeHSP17 also rescues the thermal lethality of an E. coli mutant deficient in degP, which encodes a protein quality control factor localized in the periplasmic space. Mechanistically, we show that CeHSP17 is partially localized in the periplasmic space and associated with the inner membrane of E. coli, and it helps to maintain the cell envelope integrity of the E. coli cells at the lethal temperatures. Together, our data indicate that maintaining the cell envelope integrity is crucial for the E. coli cells to grow at high temperatures and also shed new light on the development of thermophilic bacteria for industrial application.

语种英语
WOS记录号WOS:000335909500009
项目编号2012CB917300 ; 31170738 ; 31100559 ; 31270804
资助机构National Basic Research Program of China (973 Program) ; National Natural Science Foundation of China
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64772
专题北京大学基础医学院_生物物理学系
作者单位1.Peking Univ, Ctr Prot Sci, Beijing 100871, Peoples R China
2.Peking Univ, Sch Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Biophys, Beijing 100871, Peoples R China
4.Xuzhou Med Coll, Jiangsu Prov Key Lab Anesthesiol, Xuzhou, Peoples R China
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GB/T 7714
Ezemaduka, Anastasia N.,Yu, Jiayu,Shi, Xiaodong,et al. A Small Heat Shock Protein Enables Escherichia coli To Grow at a Lethal Temperature of 50 degrees C Conceivably by Maintaining Cell Envelope Integrity[J]. JOURNAL OF BACTERIOLOGY,2014,196(11):2004-2011.
APA Ezemaduka, Anastasia N..,Yu, Jiayu.,Shi, Xiaodong.,Zhang, Kaiming.,Yin, Chang-Cheng.,...&Chang, Zengyi.(2014).A Small Heat Shock Protein Enables Escherichia coli To Grow at a Lethal Temperature of 50 degrees C Conceivably by Maintaining Cell Envelope Integrity.JOURNAL OF BACTERIOLOGY,196(11),2004-2011.
MLA Ezemaduka, Anastasia N.,et al."A Small Heat Shock Protein Enables Escherichia coli To Grow at a Lethal Temperature of 50 degrees C Conceivably by Maintaining Cell Envelope Integrity".JOURNAL OF BACTERIOLOGY 196.11(2014):2004-2011.
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