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学科主题: 临床医学
题名:
Functional alpha(1)-adrenoceptor subtypes in human submandibular glands
作者: Huang, Z; Wu, LL; Zhang, YY; Gao, Y; Yu, GY
关键词: alpha(1)-adrenoceptor ; receptor subtypes ; neurotransmitter ; submandibular gland ; tissue distribution
刊名: JOURNAL OF DENTAL RESEARCH
发表日期: 2006-03-01
卷: 85, 期:3, 页:251-256
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Dentistry, Oral Surgery & Medicine
关键词[WOS]: MESSENGER-RNA ; RECEPTOR STIMULATION ; SALIVARY-GLANDS ; ACINAR-CELLS ; RAT-TISSUES ; EXPRESSION ; CLONING ; MECHANISMS ; ADRENOCEPTORS ; ALPHA(1B)
英文摘要:

alpha(1)-Adrenoceptor has been discovered to exist in many human tissues and mediates important physiological functions. The purpose of this study was to detect the expression, distribution, and function of alpha(1)-adrenoceptor subtypes in human submandibular glands. alpha(1A)-and alpha(1B)-Adrenoceptor mRNAs were identified by reverse-trans-cription/polymerase chain-reaction (RT-PCR), and their proteins were detected by Western blotting. No expression of the alpha(1D)-adrenoceptor mRNA and protein was found. By in situ hybridization and immunohistochemistry, alpha(1A)- and alpha(1B)-adrenoceptor mRNAs and proteins were shown to be widespread in both ductal and acinar cells. By confocal microscopy, phenylephrine (stimulating both alpha(1A)- and alpha(1B)-adrenoceptors) or A61603 (alpha(1A)-selective agonist) induced an increase in intracellular calcium by 2.33 +/- 0.18-fold and 1.81 +/- 0.43-fold, respectively, while 5-methylurapidil (alpha(1A)- selective antagonist) partly blocked calcium mobility stimulated by phenylephrine. The results indicated that functional alpha(1A)- and alpha(1B)-adrenoceptors were expressed in human submandibular glands, and might contribute to the regulation of saliva synthesis and secretion.

语种: 英语
WOS记录号: WOS:000236588200009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64782
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Pathophysiol, Beijing 100871, Peoples R China
2.Peking Univ, Third Hosp, Inst Vasc Med, Beijing 100871, Peoples R China
3.Peking Univ, Dept Oral Pathol, Sch & Hosp Stomatol, Beijing 100081, Peoples R China
4.Peking Univ, Dept Oral & Maxillofacial Surg, Sch & Hosp Stomatol, Beijing 100081, Peoples R China

Recommended Citation:
Huang, Z,Wu, LL,Zhang, YY,et al. Functional alpha(1)-adrenoceptor subtypes in human submandibular glands[J]. JOURNAL OF DENTAL RESEARCH,2006,85(3):251-256.
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