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Design and Synthesis of In-111-CCPM-RGD Nanoparticles for Dual-modality Molecular Imaging
Zhu Hua1; Li Nan1; Lin Xinfeng1; Hong Ye2; Yang Zhi1
关键词core-crosslinked polymeric micelles tumor target dual-modality imaging indium-111
刊名ACTA CHIMICA SINICA
2014-04-15
DOI10.6023/A13121248
72期:4页:427-432
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]POLYMERIC MICELLES ; GOLD NANOPARTICLES ; PEPTIDE ; TOMOGRAPHY ; SPECT
英文摘要

The multi-modality probes are essential for multi-modality imaging. Dual labeled imaging probes allow the same target to be evaluated with two different modalities. Based on the structure of c-RGD peptide motif; some examples of multimodality agents have been reported in design of RGD for alpha(v)beta(3) intergin positive tumor imaging. The aim of this study was to develop a dual-labeled nanoparticles for both single photon emission computed tomography (SPECT) and near-infrared fluorescence (NIRF) imaging of alpha(v)beta(3). In this paper, c-RGD was conjugated to near-infrared fluorescence fluorophores (Cy7) entrapped polyethylene glycol-coated, core-crosslinked polymeric micelles (NIRF-CCPM) labeled with a radioisotope indium 111. The labeling efficiency was over 96%, the radio chemical purity was over 99% after purification. The in vitro stability tests were presented in 5% bull serum albumin (BSA) for 72 h at 25 degrees C. Less than 10% In-111 dissociation was detected by Radio-Thin-Layer Chromatography. In-111-CCPM-RGD seems to be a promising candidate for dual optical and nuclear imaging applications in tumor detection. Its structure was fully characterized. Serial SPECT imaging of human glioma U87 tumor-bearing nude mice revealed that tumor uptake of In-111-CCPM-RGD got a substantial growth from 24 h to 72 h after post-injection of 11.1 MBq nanoparticles, corroborated by in vivo NIRF imaging. Tumor uptake as measured by in vivo NIRF imaging by region of interest (ROT) with the unit of average radiant efficiency. The ROT of tumor NIRF imaging were 4.81 X 10(7) and 6.91 X 10(7) [p/sec/cm(2)/sr]/[mu W/cm(2)] at 24 h, 72 h respectively. The in vivo SPECT imaging was general concordance with NIRF imaging. The combination of these nuclear and NIRF imaging technologies will significantly improve the accuracy in tumor diagnosis. The persistent, excellent, and alpha(v)beta(3)-specific uptake of In-111-CCPM-RGD in the tumor, observed by both SPECT and NIRF imaging, warrants further investigation and future clinical translation of such nanoparticles-based imaging agents.

语种中文
WOS记录号WOS:000336561800002
项目编号81071198 ; 81172082 ; 81371592 ; 7132040
资助机构National Natural Science Foundation of China ; Beijing Natural Science Foundation
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64792
专题北京大学临床肿瘤学院_核医学科
作者单位1.Peking Univ, Canc Hosp & Inst, Dept Nucl Med, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100142, Peoples R China
2.China Inst Atom Energy, Dept Isotope, Beijing 102413, Peoples R China
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Zhu Hua,Li Nan,Lin Xinfeng,et al. Design and Synthesis of In-111-CCPM-RGD Nanoparticles for Dual-modality Molecular Imaging[J]. ACTA CHIMICA SINICA,2014,72(4):427-432.
APA Zhu Hua,Li Nan,Lin Xinfeng,Hong Ye,&Yang Zhi.(2014).Design and Synthesis of In-111-CCPM-RGD Nanoparticles for Dual-modality Molecular Imaging.ACTA CHIMICA SINICA,72(4),427-432.
MLA Zhu Hua,et al."Design and Synthesis of In-111-CCPM-RGD Nanoparticles for Dual-modality Molecular Imaging".ACTA CHIMICA SINICA 72.4(2014):427-432.
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