IR@PKUHSC  > 北京大学深圳医院
学科主题临床医学
The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line
Xu, Xun1; Nagarajan, Harish2; Lewis, Nathan E.2; Pan, Shengkai1; Cai, Zhiming3; Liu, Xin1; Chen, Wenbin1; Xie, Min1; Wang, Wenliang1; Hammond, Stephanie4,5; Andersen, Mikael R.6; Neff, Norma7,8; Passarelli, Benedetto7,8; Koh, Winston7,8; Fan, H. Christina7,8; Wang, Jianbin7,8; Gui, Yaoting3; Lee, Kelvin H.4,5; Betenbaugh, Michael J.9,10; Quake, Stephen R.7,8; Famili, Iman2; Palsson, Bernhard O.2,10; Wang, Jun1,11,12
刊名NATURE BIOTECHNOLOGY
2011-08-01
DOI10.1038/nbt.1932
29期:8页:735-U131
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology
资助者National Natural Science Foundation (NSFC) of China ; Shenzhen government ; Guangdong Innovation Team ; National Basic Research Program of China (973 program) ; US National Institutes of Health (NIH) ; National Cancer Institute ; Danish Agency for Science, Technology and Innovation ; National Natural Science Foundation (NSFC) of China ; Shenzhen government ; Guangdong Innovation Team ; National Basic Research Program of China (973 program) ; US National Institutes of Health (NIH) ; National Cancer Institute ; Danish Agency for Science, Technology and Innovation
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]HERPES-SIMPLEX-VIRUS ; HEPARAN-SULFATE 2-SULFOTRANSFERASE ; MAMMALIAN-CELLS ; THERAPEUTIC ANTIBODIES ; ESCHERICHIA-COLI ; SIALIC ACID ; CHO-CELLS ; GLYCOSYLATION ; EXPRESSION ; PROTEINS
英文摘要

Chinese hamster ovary (CHO)-derived cell lines are the preferred host cells for the production of therapeutic proteins. Here we present a draft genomic sequence of the CHO-K1 ancestral cell line. The assembly comprises 2.45 Gb of genomic sequence, with 24,383 predicted genes. We associate most of the assembled scaffolds with 21 chromosomes isolated by microfluidics to identify chromosomal locations of genes. Furthermore, we investigate genes involved in glycosylation, which affect therapeutic protein quality, and viral susceptibility genes, which are relevant to cell engineering and regulatory concerns. Homologs of most human glycosylation-associated genes are present in the CHO-K1 genome, although 141 of these homologs are not expressed under exponential growth conditions. Many important viral entry genes are also present in the genome but not expressed, which may explain the unusual viral resistance property of CHO cell lines. We discuss how the availability of this genome sequence may facilitate genome-scale science for the optimization of biopharmaceutical protein production.

语种英语
所属项目编号30725008 ; ZYC200903240077A ; 2007CB815703 ; 2P20RR016472-10 ; NIH R44CA139977 ; 07-015498 ; CXB200903110066A
资助者National Natural Science Foundation (NSFC) of China ; Shenzhen government ; Guangdong Innovation Team ; National Basic Research Program of China (973 program) ; US National Institutes of Health (NIH) ; National Cancer Institute ; Danish Agency for Science, Technology and Innovation ; National Natural Science Foundation (NSFC) of China ; Shenzhen government ; Guangdong Innovation Team ; National Basic Research Program of China (973 program) ; US National Institutes of Health (NIH) ; National Cancer Institute ; Danish Agency for Science, Technology and Innovation
WOS记录号WOS:000293696500026
引用统计
被引频次:325[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64793
专题北京大学深圳医院
作者单位1.BGI Shenzhen, Shenzhen, Peoples R China
2.GT Life Sci, San Diego, CA USA
3.Peking Univ, Shenzhen PKU HKUST Med Ctr, Guangdong Key Lab Male Reprod Med & Genet, Shenzhen Hosp, Shenzhen, Peoples R China
4.Univ Delaware, Dept Chem Engn, Newark, DE USA
5.Univ Delaware, Delaware Biotechnol Inst, Newark, DE USA
6.Tech Univ Denmark, Ctr Microbial Biotechnol, Dept Syst Biol, DK-2800 Lyngby, Denmark
7.Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA
8.Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA
9.Johns Hopkins Univ, Dept Chem & Biomol Engn, Baltimore, MD USA
10.Tech Univ Denmark, Novo Nordisk Fdn, Ctr Biosustainabil, Horsholm, Denmark
11.Univ Copenhagen, Novo Nordisk Fdn, Ctr Basic Metab Res, Copenhagen, Denmark
12.Univ Copenhagen, Dept Biol, Copenhagen, Denmark
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GB/T 7714
Xu, Xun,Nagarajan, Harish,Lewis, Nathan E.,et al. The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line[J]. NATURE BIOTECHNOLOGY,2011,29(8):735-U131.
APA Xu, Xun.,Nagarajan, Harish.,Lewis, Nathan E..,Pan, Shengkai.,Cai, Zhiming.,...&Wang, Jun.(2011).The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line.NATURE BIOTECHNOLOGY,29(8),735-U131.
MLA Xu, Xun,et al."The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line".NATURE BIOTECHNOLOGY 29.8(2011):735-U131.
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