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学科主题: 临床医学
题名:
The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line
作者: Xu, Xun1; Nagarajan, Harish2; Lewis, Nathan E.2; Pan, Shengkai1; Cai, Zhiming3; Liu, Xin1; Chen, Wenbin1; Xie, Min1; Wang, Wenliang1; Hammond, Stephanie4,5; Andersen, Mikael R.6; Neff, Norma7,8; Passarelli, Benedetto7,8; Koh, Winston7,8; Fan, H. Christina7,8; Wang, Jianbin7,8; Gui, Yaoting3; Lee, Kelvin H.4,5; Betenbaugh, Michael J.9,10; Quake, Stephen R.7,8; Famili, Iman2; Palsson, Bernhard O.2,10; Wang, Jun1,11,12
刊名: NATURE BIOTECHNOLOGY
发表日期: 2011-08-01
DOI: 10.1038/nbt.1932
卷: 29, 期:8, 页:735-U131
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
关键词[WOS]: HERPES-SIMPLEX-VIRUS ; HEPARAN-SULFATE 2-SULFOTRANSFERASE ; MAMMALIAN-CELLS ; THERAPEUTIC ANTIBODIES ; ESCHERICHIA-COLI ; SIALIC ACID ; CHO-CELLS ; GLYCOSYLATION ; EXPRESSION ; PROTEINS
英文摘要:

Chinese hamster ovary (CHO)-derived cell lines are the preferred host cells for the production of therapeutic proteins. Here we present a draft genomic sequence of the CHO-K1 ancestral cell line. The assembly comprises 2.45 Gb of genomic sequence, with 24,383 predicted genes. We associate most of the assembled scaffolds with 21 chromosomes isolated by microfluidics to identify chromosomal locations of genes. Furthermore, we investigate genes involved in glycosylation, which affect therapeutic protein quality, and viral susceptibility genes, which are relevant to cell engineering and regulatory concerns. Homologs of most human glycosylation-associated genes are present in the CHO-K1 genome, although 141 of these homologs are not expressed under exponential growth conditions. Many important viral entry genes are also present in the genome but not expressed, which may explain the unusual viral resistance property of CHO cell lines. We discuss how the availability of this genome sequence may facilitate genome-scale science for the optimization of biopharmaceutical protein production.

语种: 英语
所属项目编号: 30725008 ; ZYC200903240077A ; 2007CB815703 ; 2P20RR016472-10 ; NIH R44CA139977 ; 07-015498 ; CXB200903110066A
项目资助者: National Natural Science Foundation (NSFC) of China ; Shenzhen government ; Guangdong Innovation Team ; National Basic Research Program of China (973 program) ; US National Institutes of Health (NIH) ; National Cancer Institute ; Danish Agency for Science, Technology and Innovation
WOS记录号: WOS:000293696500026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64793
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.BGI Shenzhen, Shenzhen, Peoples R China
2.GT Life Sci, San Diego, CA USA
3.Peking Univ, Shenzhen PKU HKUST Med Ctr, Guangdong Key Lab Male Reprod Med & Genet, Shenzhen Hosp, Shenzhen, Peoples R China
4.Univ Delaware, Dept Chem Engn, Newark, DE USA
5.Univ Delaware, Delaware Biotechnol Inst, Newark, DE USA
6.Tech Univ Denmark, Ctr Microbial Biotechnol, Dept Syst Biol, DK-2800 Lyngby, Denmark
7.Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA
8.Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA
9.Johns Hopkins Univ, Dept Chem & Biomol Engn, Baltimore, MD USA
10.Tech Univ Denmark, Novo Nordisk Fdn, Ctr Biosustainabil, Horsholm, Denmark
11.Univ Copenhagen, Novo Nordisk Fdn, Ctr Basic Metab Res, Copenhagen, Denmark
12.Univ Copenhagen, Dept Biol, Copenhagen, Denmark

Recommended Citation:
Xu, Xun,Nagarajan, Harish,Lewis, Nathan E.,et al. The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line[J]. NATURE BIOTECHNOLOGY,2011,29(8):735-U131.
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