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学科主题: 临床医学
题名:
Assessment of the Trueness and Inter-Laboratory Precision of Routine Uric Acid Assays Using 4 Frozen Pooled Serum Samples Measured by the Japan Society of Clinical Chemistry′s HPLC Method
作者: Jiang, Yaping1; Liu, Ou2; Xu, Guobing1
关键词: Uric acid ; HPLC ; Standardization
刊名: ANNALS OF LABORATORY MEDICINE
发表日期: 2014-03-01
DOI: 10.3343/alm.2014.34.2.104
卷: 34, 期:2, 页:104-110
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medical Laboratory Technology
研究领域[WOS]: Medical Laboratory Technology
关键词[WOS]: CANDIDATE REFERENCE METHOD ; DILUTION MASS-SPECTROMETRY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; HYPERURICEMIA
英文摘要:

Background: Reference procedures are required for evaluating the accuracy of routine analytical systems for uric acid (UA). External quality assessment (EQA) for UA has only been conducted with quality controls in China, and the results have not been published. This study was designed to investigate both the trueness and inter-laboratory precision of UA measurements among routine analytical systems using a candidate reference method.

Methods: We performed the HPLC method recommended by the Japan Society of Clinical Chemistry (JSCC). Next, we evaluated its analytical performance and validated its trueness. The performance of 4 routine analytical systems (5 instruments per system, n = 20) for UA was assessed by using 4 frozen pooled serum samples measured by the HPLC method according to biologically relevant quality goals.

Results: Within-run, between-run, inter-day, and total CV of the method were less than 0.3%, 0.4%, 1.8%, and 2.6%, respectively. The UA measurements were consistent with the target values of standard reference material (SRM) 909b, the sixth ring trial for Reference Laboratories (RELA-2008) specimen, and national primary reference materials. The 4 frozen pooled serum samples were homogeneous, stable, and commutable. All routine systems achieved the desirable performance goal (total error < 11.9%).

Conclusions: We successfully reproduced the JSCC′s HPLC method, which was simple, specific, precise, and accurate. We recommend this method as a reference method for UA measurement in human serum. Four routine analytical systems for UA measurement had acceptable traceability, and their UA results showed good concordance.

语种: 英语
所属项目编号: 2009-1012
项目资助者: Capital Medical Development Fund Projects of China
WOS记录号: WOS:000332681100004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64796
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Dept Clin Lab, Beijing, Peoples R China
2.Tsinghua Univ, Hosp 1, Dept Clin Lab, Beijing, Peoples R China

Recommended Citation:
Jiang, Yaping,Liu, Ou,Xu, Guobing. Assessment of the Trueness and Inter-Laboratory Precision of Routine Uric Acid Assays Using 4 Frozen Pooled Serum Samples Measured by the Japan Society of Clinical Chemistry&prime;s HPLC Method[J]. ANNALS OF LABORATORY MEDICINE,2014,34(2):104-110.
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