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Assessment of the Trueness and Inter-Laboratory Precision of Routine Uric Acid Assays Using 4 Frozen Pooled Serum Samples Measured by the Japan Society of Clinical Chemistry′s HPLC Method
Jiang, Yaping1; Liu, Ou2; Xu, Guobing1
关键词Uric acid HPLC Standardization
刊名ANNALS OF LABORATORY MEDICINE
2014-03-01
DOI10.3343/alm.2014.34.2.104
34期:2页:104-110
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medical Laboratory Technology
资助者Capital Medical Development Fund Projects of China ; Capital Medical Development Fund Projects of China
研究领域[WOS]Medical Laboratory Technology
关键词[WOS]CANDIDATE REFERENCE METHOD ; DILUTION MASS-SPECTROMETRY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; HYPERURICEMIA
英文摘要

Background: Reference procedures are required for evaluating the accuracy of routine analytical systems for uric acid (UA). External quality assessment (EQA) for UA has only been conducted with quality controls in China, and the results have not been published. This study was designed to investigate both the trueness and inter-laboratory precision of UA measurements among routine analytical systems using a candidate reference method.

Methods: We performed the HPLC method recommended by the Japan Society of Clinical Chemistry (JSCC). Next, we evaluated its analytical performance and validated its trueness. The performance of 4 routine analytical systems (5 instruments per system, n = 20) for UA was assessed by using 4 frozen pooled serum samples measured by the HPLC method according to biologically relevant quality goals.

Results: Within-run, between-run, inter-day, and total CV of the method were less than 0.3%, 0.4%, 1.8%, and 2.6%, respectively. The UA measurements were consistent with the target values of standard reference material (SRM) 909b, the sixth ring trial for Reference Laboratories (RELA-2008) specimen, and national primary reference materials. The 4 frozen pooled serum samples were homogeneous, stable, and commutable. All routine systems achieved the desirable performance goal (total error < 11.9%).

Conclusions: We successfully reproduced the JSCC′s HPLC method, which was simple, specific, precise, and accurate. We recommend this method as a reference method for UA measurement in human serum. Four routine analytical systems for UA measurement had acceptable traceability, and their UA results showed good concordance.

语种英语
所属项目编号2009-1012
资助者Capital Medical Development Fund Projects of China ; Capital Medical Development Fund Projects of China
WOS记录号WOS:000332681100004
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64796
专题北京大学第一临床医学院
作者单位1.Peking Univ, Hosp 1, Dept Clin Lab, Beijing, Peoples R China
2.Tsinghua Univ, Hosp 1, Dept Clin Lab, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Jiang, Yaping,Liu, Ou,Xu, Guobing. Assessment of the Trueness and Inter-Laboratory Precision of Routine Uric Acid Assays Using 4 Frozen Pooled Serum Samples Measured by the Japan Society of Clinical Chemistry&prime;s HPLC Method[J]. ANNALS OF LABORATORY MEDICINE,2014,34(2):104-110.
APA Jiang, Yaping,Liu, Ou,&Xu, Guobing.(2014).Assessment of the Trueness and Inter-Laboratory Precision of Routine Uric Acid Assays Using 4 Frozen Pooled Serum Samples Measured by the Japan Society of Clinical Chemistry′s HPLC Method.ANNALS OF LABORATORY MEDICINE,34(2),104-110.
MLA Jiang, Yaping,et al."Assessment of the Trueness and Inter-Laboratory Precision of Routine Uric Acid Assays Using 4 Frozen Pooled Serum Samples Measured by the Japan Society of Clinical Chemistry′s HPLC Method".ANNALS OF LABORATORY MEDICINE 34.2(2014):104-110.
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