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学科主题: 临床医学
题名:
The transport of alpha(1A)-adrenergic receptor with 33-nm step size in live cells
作者: Liang, Zhang-Yi; Xu, Ning; Guan, Ying-Hua; Xu, Ming; He, Qi-Hua; Han, Qi-De; Zhang, You-Yi; Zhao, Xin-Sheng
关键词: alpha(1A)-adrenergic receptor ; transport ; living cell ; actin ; real time ; single particle tracking ; myosin
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2007-02-09
DOI: 10.1016/j.bbrc.2006.11.116
卷: 353, 期:2, 页:231-237
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: PROTEIN-COUPLED RECEPTORS ; HAND-OVER-HAND ; MYOSIN-V ; ALPHA(1)-ADRENOCEPTOR SUBTYPES ; MEDIATED INTERNALIZATION ; ACTIN ; ALPHA(1B)-ADRENOCEPTOR ; DESENSITIZATION ; LOCALIZATION ; MECHANISM
英文摘要:

We used the technique of single particle tracking (SPT) with high tempo-spatial resolution to efficiently explore the route and mechanism for the transport Of alpha(1A)-adrenergic receptor (alpha(1A)-AR) in real time in living cells. We found that the initial transport Of alpha(1A)-AR in cells depended on actin filaments with the velocity of 0.2 mu m/s and exhibited discrete 33-nm steps. It was noted that the step size, the rate constant, and the velocities were in accordance with the character of single myosin in vitro, implying that while transporting each endosome myosins did not work in the "tug-of-war" mode and that they did not adopt the strategy to boost up transporting speed by working coordinately. These results provided insight into the mechanism of GPCR transport ill vivo. (c) 2006 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000243543100003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64803
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: 1.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China
2.Peking Univ, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100871, Peoples R China
3.Peking Univ, Dept Biol Chem, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
4.Peking Univ Third Hosp, Inst Vasc Med, Beijing 100083, Peoples R China
5.Peking Univ, Ctr Med Anal, Hlth Sci Ctr, Beijing 100083, Peoples R China

Recommended Citation:
Liang, Zhang-Yi,Xu, Ning,Guan, Ying-Hua,et al. The transport of alpha(1A)-adrenergic receptor with 33-nm step size in live cells[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2007,353(2):231-237.
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