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学科主题: 临床医学
题名:
Heterotopic ossification induced by Achilles tenotomy via endochondral bone formation: Expression of bone and cartilage related genes
作者: Lin, Lin1; Shen, Qi2; Xue, Tao1; Yu, Changlong1
关键词: Heterotopic ossification ; Chondrogenesis ; Endochondral ossification ; HIF-1 alpha ; Achilles tenotomy
刊名: BONE
发表日期: 2010-02-01
DOI: 10.1016/j.bone.2009.08.057
卷: 46, 期:2, 页:425-431
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Endocrinology & Metabolism
研究领域[WOS]: Endocrinology & Metabolism
关键词[WOS]: GROWTH-FACTOR-BETA ; ANIMAL-MODEL ; IN-VITRO ; TGF-BETA ; OSTEOBLAST DIFFERENTIATION ; TRANSCRIPTIONAL ACTIVATION ; MORPHOGENETIC PROTEIN-2 ; DISTINCT MECHANISMS ; MESENCHYMAL CELLS ; HYPOXIA
英文摘要:

Animal model for heterotopic ossification (HO) induced by Achilles tenotomy in rats has been used in the literature. However, the molecular mechanism remains unclear. Here, we studied bone and cartilage related genes and their possible roles in this model. Thirty rats underwent bilateral midpoint Achilles tenotomy through a posterior approach under aseptic condition. At 3, 5 and 10 weeks post-operation, Xray and histological examinations were carried out to investigate the formation of HO. At different phases of HO formation, mRNA levels of transforming growth factor (TGF)-beta 1, TGF-beta 2, TGF-beta 3, bone morphogenetic proteins (BMP)-2, BMP-4, BMP-7, hypoxia inducible factor (HIF)-1 alpha, Sox9, Runx2, vascular endothelial growth factor (VEGF), aggrecan and collagen type I, II and X were evaluated by real-time RTPCR. Protein levels of TGF-beta 1, TGF-beta 2, TGF-beta 3. BMP-2. BMP-4, BMP-7, HIF-1 alpha, Sox9 and Runx2 were also examined by immunohistochemical staining. During the chondrogenic phase, the expressions of HIF-1 alpha and Sox9 were significantly up-regulated. Runx2 expression was significantly up-regulated during osteogenic phase, while HIF-1 alpha and Sox9 expression was significant decreased. TGF-beta 1 mRNA levels were rather constant, and the mRNA levels of TGF-beta 2, TGF-beta 3 and BMPs were changed throughout HO formation. The presences of the proteins of HIF-1 alpha, Sox9, Runx2, TGF-beta s and BMPs within the HO tissues were confirmed by immunohistochemical staining. Our study indicates that HO induced by Achilles tenotomy is by endochondral bone formation, and HIF-1 alpha activation plays an important role during chondrogenesis in this model. Furthermore, the model provides a new experimental system to study endochondral ossification. (C) 2009 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 20081304
项目资助者: Beijing Municipal Science and Technology Commission and Doctoral Program Foundation of Institutions of Higher Education of China
WOS记录号: WOS:000274702800025
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64806
Appears in Collections:北京大学第三临床医学院_运动医学研究所_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Inst Urol, Beijing 100034, Peoples R China
2.Peking Univ, Hosp 3, Inst Sports Med, Beijing 100191, Peoples R China

Recommended Citation:
Lin, Lin,Shen, Qi,Xue, Tao,et al. Heterotopic ossification induced by Achilles tenotomy via endochondral bone formation: Expression of bone and cartilage related genes[J]. BONE,2010,46(2):425-431.
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