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学科主题: 基础医学
题名:
Effects of Gekko sulfated polysaccharide-protein complex on human hepatoma SMMC-7721 cells: Inhibition of proliferation and migration
作者: Chen, Dan2; Yao, Wei-Juan2; Zhang, Xiao-Lan2; Han, Xiao-Qiang3; Qu, Xiao-Yuan3; Ka, Wei-Bo2; Sun, Da-Gong2; Wu, Xiong-Zhi1; Wen, Zong-Yao2
关键词: Gekko swinhonis Guenther ; Sulfated polysaccharide ; Hepatocellular carcinoma ; Calcium ; Actin ; Migration
刊名: JOURNAL OF ETHNOPHARMACOLOGY
发表日期: 2010-02-17
DOI: 10.1016/j.jep.2009.12.003
卷: 127, 期:3, 页:702-708
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
研究领域[WOS]: Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
关键词[WOS]: ACTIN REORGANIZATION ; HEPARANASE ACTIVITY ; TUMOR ANGIOGENESIS ; REPEATING UNIT ; IN-VITRO ; CANCER ; METASTASIS ; GROWTH ; CYTOSKELETON ; CARCINOMA
英文摘要:

Aim of the study: Gekko swinhonis Guenther has been used as an anti-cancer drug in traditional Chinese medicine for hundreds of years. Here we investigated the structural characterization and anti-cancer effects of sulfated polysaccharide-protein complex (GSPP) isolated from Gekko swinhonis Guenther.

Materials and methods: The structure of GSPP was characterized by high performance liquid chromatography, gas chromatography, gas chromatography-mass spectrometry, beta-elimination reaction, and NMR spectroscopy. SMMC-7721 cells were used to assess the influence of GSPP on hepatocellular carcinoma. Cell proliferation and survival was determined by trypan blue exclusion assay. Cell migration was performed by wound-healing and transwell assay. The secretion of IL-8 was detected by an enzyme-linked immumosorbent assay kit. Flow cytometry was used to analyze intracellular calcium concentration, as well as cell cycle distribution and apoptosis. Confocal microscopy was used to assess the localization and configuration of actin filaments.

Results: GSPP was chemically characterized as a sulfated polysaccharide-protein complex with O-glycopeptide linkages. Our results showed that GSPP inhibited the proliferation of SMMC-7721 cells and blocked cells in the S phase. No direct toxicity against cells was observed. Furthermore, GSPP inhibited the migration of SMMC-7721 cells with the reduction of intracellular calcium. Actin filaments were polymerized and accumulated in the cytoplasm of the treated cells, whereas the secretion of IL-8 was not significantly changed after GSPP exposure.

Conclusion: We describe an identified sulfated polysaccharide-protein complex, and demonstrate its direct effect on hepatocellular carcinoma cell migration via calcium-mediated regulation of the actin cytoskeleton reorganization. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
所属项目编号: 30770532 ; 30801495
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000275628600018
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64809
Appears in Collections:基础医学院_生物物理学系_期刊论文

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作者单位: 1.Tianjin Med Univ, Canc Inst & Hosp, Zhong Shan Men In Patient Dept, Tianjin 300060, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Med Phys, Sch Basic Med Sci,Hemorheol Ctr, Beijing 100191, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Modern Res Ctr Tradit Chinese Med, Beijing 100191, Peoples R China

Recommended Citation:
Chen, Dan,Yao, Wei-Juan,Zhang, Xiao-Lan,et al. Effects of Gekko sulfated polysaccharide-protein complex on human hepatoma SMMC-7721 cells: Inhibition of proliferation and migration[J]. JOURNAL OF ETHNOPHARMACOLOGY,2010,127(3):702-708.
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