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学科主题基础医学
Effects of Gekko sulfated polysaccharide-protein complex on human hepatoma SMMC-7721 cells: Inhibition of proliferation and migration
Chen, Dan2; Yao, Wei-Juan2; Zhang, Xiao-Lan2; Han, Xiao-Qiang3; Qu, Xiao-Yuan3; Ka, Wei-Bo2; Sun, Da-Gong2; Wu, Xiong-Zhi1; Wen, Zong-Yao2
关键词Gekko swinhonis Guenther Sulfated polysaccharide Hepatocellular carcinoma Calcium Actin Migration
刊名JOURNAL OF ETHNOPHARMACOLOGY
2010-02-17
DOI10.1016/j.jep.2009.12.003
127期:3页:702-708
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
研究领域[WOS]Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
关键词[WOS]ACTIN REORGANIZATION ; HEPARANASE ACTIVITY ; TUMOR ANGIOGENESIS ; REPEATING UNIT ; IN-VITRO ; CANCER ; METASTASIS ; GROWTH ; CYTOSKELETON ; CARCINOMA
英文摘要

Aim of the study: Gekko swinhonis Guenther has been used as an anti-cancer drug in traditional Chinese medicine for hundreds of years. Here we investigated the structural characterization and anti-cancer effects of sulfated polysaccharide-protein complex (GSPP) isolated from Gekko swinhonis Guenther.

Materials and methods: The structure of GSPP was characterized by high performance liquid chromatography, gas chromatography, gas chromatography-mass spectrometry, beta-elimination reaction, and NMR spectroscopy. SMMC-7721 cells were used to assess the influence of GSPP on hepatocellular carcinoma. Cell proliferation and survival was determined by trypan blue exclusion assay. Cell migration was performed by wound-healing and transwell assay. The secretion of IL-8 was detected by an enzyme-linked immumosorbent assay kit. Flow cytometry was used to analyze intracellular calcium concentration, as well as cell cycle distribution and apoptosis. Confocal microscopy was used to assess the localization and configuration of actin filaments.

Results: GSPP was chemically characterized as a sulfated polysaccharide-protein complex with O-glycopeptide linkages. Our results showed that GSPP inhibited the proliferation of SMMC-7721 cells and blocked cells in the S phase. No direct toxicity against cells was observed. Furthermore, GSPP inhibited the migration of SMMC-7721 cells with the reduction of intracellular calcium. Actin filaments were polymerized and accumulated in the cytoplasm of the treated cells, whereas the secretion of IL-8 was not significantly changed after GSPP exposure.

Conclusion: We describe an identified sulfated polysaccharide-protein complex, and demonstrate its direct effect on hepatocellular carcinoma cell migration via calcium-mediated regulation of the actin cytoskeleton reorganization. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000275628600018
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被引频次:32[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64809
专题北京大学基础医学院_生物物理学系
北京大学基础医学院
医学人文研究院/公共教学部_哲学与社会科学系
作者单位1.Tianjin Med Univ, Canc Inst & Hosp, Zhong Shan Men In Patient Dept, Tianjin 300060, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Med Phys, Sch Basic Med Sci,Hemorheol Ctr, Beijing 100191, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Modern Res Ctr Tradit Chinese Med, Beijing 100191, Peoples R China
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Chen, Dan,Yao, Wei-Juan,Zhang, Xiao-Lan,et al. Effects of Gekko sulfated polysaccharide-protein complex on human hepatoma SMMC-7721 cells: Inhibition of proliferation and migration[J]. JOURNAL OF ETHNOPHARMACOLOGY,2010,127(3):702-708.
APA Chen, Dan.,Yao, Wei-Juan.,Zhang, Xiao-Lan.,Han, Xiao-Qiang.,Qu, Xiao-Yuan.,...&Wen, Zong-Yao.(2010).Effects of Gekko sulfated polysaccharide-protein complex on human hepatoma SMMC-7721 cells: Inhibition of proliferation and migration.JOURNAL OF ETHNOPHARMACOLOGY,127(3),702-708.
MLA Chen, Dan,et al."Effects of Gekko sulfated polysaccharide-protein complex on human hepatoma SMMC-7721 cells: Inhibition of proliferation and migration".JOURNAL OF ETHNOPHARMACOLOGY 127.3(2010):702-708.
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