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学科主题: 临床医学
题名:
Label-free detection of target DNA sequence and single-base mismatch in hepatitis C virus corresponding to oligonucleotide by resonance light scattering technique
作者: Chen, Zhanguang1; Qian, Sihua1; Chen, Xi2; Chen, Junhui3; Lin, Yuejuan1; Liu, Jinbin4
刊名: RSC ADVANCES
发表日期: 2012
DOI: 10.1039/c2ra01141g
卷: 2, 期:6, 页:2562-2567
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
关键词[WOS]: ATOMIC-FORCE MICROSCOPY ; FLOW-INJECTION ANALYSIS ; NUCLEIC-ACIDS ; RAYLEIGH-SCATTERING ; CARBON NANOTUBES ; SENSITIVE ASSAY ; HOECHST-33258 ; HYBRIDIZATION ; ELECTRODES ; PARTICLES
英文摘要:

A highly sensitive assay to detect sequence-specific DNA by the resonance light scattering (RLS) technique has been developed based on the enhanced RLS intensities of the RLS spectra at 399.5 nm. It was found that Hoechst 33258, when bound to dsDNA and ssDNA in electrolyte solution, displayed different RLS signals. This encouraged us to perform a direct and unlabelled sequence-specific DNA assay. At optimal conditions, this RLS assay can detect complementary target DNA over a range of 4.0 x 10(-9) -3.8 x 10(-7) mol L-1, and the limit of detection was around 1.7 x 10(-9) mol L-1. Additionally, the results of experiments showed that different RLS signals reflected a different degree of mismatch between probe DNA and target DNA, and mismatched variants of target DNA with single-base difference can even be well discriminated. Herein, we moved the RLS technique one-step further toward sensitivity, rapidity, simplicity and non-toxicity. The RLS assay results were identified with a fluorescence method, fluorescence polarization assay and atomic force microscope measurement.

语种: 英语
所属项目编号: 9151022501000019
项目资助者: Natural Science Foundation of Guangdong Province
WOS记录号: WOS:000300828400063
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64811
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Shantou Univ, Dept Chem, Shantou 515063, Peoples R China
2.Univ Texas Dallas, Dept Chem, Richardson, TX 75080 USA
3.Guangdong Pharmaceut Univ, Guangzhou 510006, Guangdong, Peoples R China
4.Peking Univ, Shenzhen Hosp, Intervent Oncol & Minimally Invas Therapies Dept, Shenzhen 518036, Peoples R China

Recommended Citation:
Chen, Zhanguang,Qian, Sihua,Chen, Xi,et al. Label-free detection of target DNA sequence and single-base mismatch in hepatitis C virus corresponding to oligonucleotide by resonance light scattering technique[J]. RSC ADVANCES,2012,2(6):2562-2567.
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