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Label-free detection of target DNA sequence and single-base mismatch in hepatitis C virus corresponding to oligonucleotide by resonance light scattering technique
Chen, Zhanguang1; Qian, Sihua1; Chen, Xi2; Chen, Junhui3; Lin, Yuejuan1; Liu, Jinbin4
刊名RSC ADVANCES
2012
DOI10.1039/c2ra01141g
2期:6页:2562-2567
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]ATOMIC-FORCE MICROSCOPY ; FLOW-INJECTION ANALYSIS ; NUCLEIC-ACIDS ; RAYLEIGH-SCATTERING ; CARBON NANOTUBES ; SENSITIVE ASSAY ; HOECHST-33258 ; HYBRIDIZATION ; ELECTRODES ; PARTICLES
英文摘要

A highly sensitive assay to detect sequence-specific DNA by the resonance light scattering (RLS) technique has been developed based on the enhanced RLS intensities of the RLS spectra at 399.5 nm. It was found that Hoechst 33258, when bound to dsDNA and ssDNA in electrolyte solution, displayed different RLS signals. This encouraged us to perform a direct and unlabelled sequence-specific DNA assay. At optimal conditions, this RLS assay can detect complementary target DNA over a range of 4.0 x 10(-9) -3.8 x 10(-7) mol L-1, and the limit of detection was around 1.7 x 10(-9) mol L-1. Additionally, the results of experiments showed that different RLS signals reflected a different degree of mismatch between probe DNA and target DNA, and mismatched variants of target DNA with single-base difference can even be well discriminated. Herein, we moved the RLS technique one-step further toward sensitivity, rapidity, simplicity and non-toxicity. The RLS assay results were identified with a fluorescence method, fluorescence polarization assay and atomic force microscope measurement.

语种英语
WOS记录号WOS:000300828400063
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64811
专题北京大学深圳医院
作者单位1.Shantou Univ, Dept Chem, Shantou 515063, Peoples R China
2.Univ Texas Dallas, Dept Chem, Richardson, TX 75080 USA
3.Guangdong Pharmaceut Univ, Guangzhou 510006, Guangdong, Peoples R China
4.Peking Univ, Shenzhen Hosp, Intervent Oncol & Minimally Invas Therapies Dept, Shenzhen 518036, Peoples R China
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GB/T 7714
Chen, Zhanguang,Qian, Sihua,Chen, Xi,et al. Label-free detection of target DNA sequence and single-base mismatch in hepatitis C virus corresponding to oligonucleotide by resonance light scattering technique[J]. RSC ADVANCES,2012,2(6):2562-2567.
APA Chen, Zhanguang,Qian, Sihua,Chen, Xi,Chen, Junhui,Lin, Yuejuan,&Liu, Jinbin.(2012).Label-free detection of target DNA sequence and single-base mismatch in hepatitis C virus corresponding to oligonucleotide by resonance light scattering technique.RSC ADVANCES,2(6),2562-2567.
MLA Chen, Zhanguang,et al."Label-free detection of target DNA sequence and single-base mismatch in hepatitis C virus corresponding to oligonucleotide by resonance light scattering technique".RSC ADVANCES 2.6(2012):2562-2567.
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