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学科主题临床医学
Resonance light scattering technique as a new tool to determine the binding mode of anticancer drug oridonin to DNA
Chen, Zhanguang1; Wang, Zhen1; Chen, Junhui2; Chen, Xi1,3,4; Wu, Jiahui1; Wu, Yanyu1; Liang, Jieyao1
关键词Oridonin Resonance light scattering DNA Intracellular target Binding mode
刊名EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
2013-08-01
DOI10.1016/j.ejmech.2013.05.042
66页:380-387
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Medicinal
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]IN-VITRO ; CANCER CELLS ; APOPTOSIS ; NANOPARTICLES ; AGGREGATION ; DERIVATIVES ; COMPLEXES ; COMPOUND ; PROTEINS ; PATHWAYS
英文摘要

In this study, a sensitive and reliable assay has been developed to determine the binding mode of oridonin to DNA in vitro using resonance light scattering (RLS) technique. The binding mode of ORI with DNA has been confirmed by measuring the changes of RLS intensity under different pH and ionic strength. It is the ORI that is intercalated into the double helix DNA to destroy its template function and inhibit the synthesis of DNA. Moreover, the RLS assay result was validated by seasoned vitro methods. The proposed RLS assay is not only an unprecedented one for primarily determine the binding mode of oridonin to DNA, but also a simple, sensitive, objective and straightforward method for the detection of ORI. (C) 2013 Elsevier Masson SAS. All rights reserved.

语种英语
WOS记录号WOS:000323628500040
项目编号9151022501000019
资助机构Natural Science Foundation of Guangdong Province
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64817
专题北京大学深圳医院
作者单位1.Shantou Univ, Dept Chem, Shantou 515063, Peoples R China
2.Peking Univ, Shenzhen Hosp, Intervent Oncol & Minimally Invas Therapies Dept, Shenzhen 518036, Peoples R China
3.Guangdong Pharmaceut Univ, Coll Tradit Med, Guangzhou 510006, Guangdong, Peoples R China
4.Sun Yat Sen Univ, Affiliated Shantou Hosp, Shantou Cent Hosp, Shantou 515031, Peoples R China
推荐引用方式
GB/T 7714
Chen, Zhanguang,Wang, Zhen,Chen, Junhui,et al. Resonance light scattering technique as a new tool to determine the binding mode of anticancer drug oridonin to DNA[J]. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY,2013,66:380-387.
APA Chen, Zhanguang.,Wang, Zhen.,Chen, Junhui.,Chen, Xi.,Wu, Jiahui.,...&Liang, Jieyao.(2013).Resonance light scattering technique as a new tool to determine the binding mode of anticancer drug oridonin to DNA.EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY,66,380-387.
MLA Chen, Zhanguang,et al."Resonance light scattering technique as a new tool to determine the binding mode of anticancer drug oridonin to DNA".EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY 66(2013):380-387.
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