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学科主题基础医学
A method to detect major serotypes of foot-and-mouth disease virus
Collins, RA; Ko, LS; Fung, KY; Lau, LT; Xing, J; Yu, ACH
关键词nucleic acid sequence-based amplification foot-and-mouth disease electrochemiluminescence internal ribosome entry site
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2002-09-20
297期:2页:267-274
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]SEQUENCE-BASED AMPLIFICATION ; POLYMERASE CHAIN-REACTION ; RT-PCR ; CYTOMEGALOVIRUS-INFECTION ; REVERSE-TRANSCRIPTASE ; NASBA TECHNOLOGY ; MESSENGER-RNA ; BLOOD-SAMPLES ; PROTEIN ; DIAGNOSIS
英文摘要

Nucleic acid sequence-based amplification (NASBA) is an isothermal technique that allows the rapid amplification of specific regions of nucleic acid obtained from a diverse range of sources. It is especially suitable for amplifying RNA sequences. A rapid and specific NASBA technique was developed, allowing the detection of foot-and-mouth disease virus genetic material in a range of sample material, including preserved skin biopsy material from infected animals, vaccines prepared from denatured cell-free material, and cell-free antigen-based detection kits. A single pair of DNA oligonuelcotide primers was able to amplify examples of all major FMD virus subtypes. The amplified viral RNA was detected by electrochemiluminescence. The method was at least as sensitive as existing cell-free antigen detection methods. (C) 2002 Elsevier Science (USA). All rights reserved.

语种英语
WOS记录号WOS:000178380400017
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被引频次:23[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64828
专题北京大学基础医学院_神经生物学系
作者单位1.Hong Kong DNA Chips Ltd, Kowloon, Hong Kong, Peoples R China
2.Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
3.Peking Univ, Dept Neurobiol, Beijing 100083, Peoples R China
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Collins, RA,Ko, LS,Fung, KY,et al. A method to detect major serotypes of foot-and-mouth disease virus[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2002,297(2):267-274.
APA Collins, RA,Ko, LS,Fung, KY,Lau, LT,Xing, J,&Yu, ACH.(2002).A method to detect major serotypes of foot-and-mouth disease virus.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,297(2),267-274.
MLA Collins, RA,et al."A method to detect major serotypes of foot-and-mouth disease virus".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 297.2(2002):267-274.
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