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学科主题: 基础医学
题名:
A method to detect major serotypes of foot-and-mouth disease virus
作者: Collins, RA; Ko, LS; Fung, KY; Lau, LT; Xing, J; Yu, ACH
关键词: nucleic acid sequence-based amplification ; foot-and-mouth disease ; electrochemiluminescence ; internal ribosome entry site
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2002-09-20
卷: 297, 期:2, 页:267-274
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: SEQUENCE-BASED AMPLIFICATION ; POLYMERASE CHAIN-REACTION ; RT-PCR ; CYTOMEGALOVIRUS-INFECTION ; REVERSE-TRANSCRIPTASE ; NASBA TECHNOLOGY ; MESSENGER-RNA ; BLOOD-SAMPLES ; PROTEIN ; DIAGNOSIS
英文摘要:

Nucleic acid sequence-based amplification (NASBA) is an isothermal technique that allows the rapid amplification of specific regions of nucleic acid obtained from a diverse range of sources. It is especially suitable for amplifying RNA sequences. A rapid and specific NASBA technique was developed, allowing the detection of foot-and-mouth disease virus genetic material in a range of sample material, including preserved skin biopsy material from infected animals, vaccines prepared from denatured cell-free material, and cell-free antigen-based detection kits. A single pair of DNA oligonuelcotide primers was able to amplify examples of all major FMD virus subtypes. The amplified viral RNA was detected by electrochemiluminescence. The method was at least as sensitive as existing cell-free antigen detection methods. (C) 2002 Elsevier Science (USA). All rights reserved.

语种: 英语
WOS记录号: WOS:000178380400017
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64828
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: 1.Hong Kong DNA Chips Ltd, Kowloon, Hong Kong, Peoples R China
2.Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
3.Peking Univ, Dept Neurobiol, Beijing 100083, Peoples R China

Recommended Citation:
Collins, RA,Ko, LS,Fung, KY,et al. A method to detect major serotypes of foot-and-mouth disease virus[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2002,297(2):267-274.
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