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学科主题: 基础医学
题名:
Molecular evolution of the vertebrate hexokinase gene family: Identification of a conserved fifth vertebrate hexokinase gene
作者: Irwin, David M.1; Tan, Huanran2
关键词: glucokinase ; gene duplication ; hexokinase ; gene evolution
刊名: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
发表日期: 2008-03-01
DOI: 10.1016/j.cbd.2007.11.002
卷: 3, 期:1, 页:96-107
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Genetics & Heredity
研究领域[WOS]: Biochemistry & Molecular Biology ; Genetics & Heredity
关键词[WOS]: C-TERMINAL HALVES ; GLUCOSE PHOSPHORYLATING ISOENZYMES ; HUMAN GLUCOKINASE GENE ; BETA-CELL GLUCOKINASE ; RAT-BRAIN HEXOKINASE ; MAMMALIAN HEXOKINASES ; FUNCTIONAL-ORGANIZATION ; DEVELOPMENTAL EXPRESSION ; CATALYTIC-ACTIVITY ; SUGAR SPECIFICITY
英文摘要:

Hexokinases (HK) phosphorylate sugar immediately upon its entry into cells allowing these sugars to be metabolized. A total of four hexokinases have been characterized in a diversity of vertebrates-HKI, HKII, HKIII, and HKIV HKIV is often called glucokinase (GCK) and has half the molecular weight of the other hexokinases, as it only has one hexokinase domain, while other vertebrate HKs have two. Differing hypothesis has been proposed to explain the diversification of the hexokinase gene family. We used a genomic approach to characterize hexokinase genes in a diverse array of vertebrate species and close relatives. Surprisingly we identified a fifth hexokinase-like gene, HKDC1 that exists and is expressed in diverse vertebrates. Analysis of the amino acid sequence of HKDC1 suggests that it may function as a hexokinase. To understand the evolution of the vertebrate hexokinase gene family we established a phylogeny of the hexokinase domain in all of the vertebrate hexokinase genes, as well as hexokinase genes from close relatives of the vertebrates. Our phylogeny demonstrates that duplication of the hexokinase domain, yielding a HK with two hexokinase domains, occurred prior to the diversification of the hexokinase gene family. We also establish that GCK evolved from a two hexokinase domain-containing gene, but has lost its N-terminal hexokinase domain. We also show that parallel changes in enzymatic function of HKI and HKIII have occurred. (C) 2007 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000254422700009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64830
Appears in Collections:基础医学院_药理学系_期刊论文

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作者单位: 1.Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON M5G 1L5, Canada
2.Peking Univ, Hlth Sci Ctr, Dept Pharmacol, Beijing 100871, Peoples R China

Recommended Citation:
Irwin, David M.,Tan, Huanran. Molecular evolution of the vertebrate hexokinase gene family: Identification of a conserved fifth vertebrate hexokinase gene[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2008,3(1):96-107.
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