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Endothelium-independent hypoxic contraction of porcine coronary arteries may be mediated by activation of phosphoinositide 3-kinase/Akt pathway
Liu, Huixia1,3; Chen, Zhengju1; Liu, Juan1; Liu, Limei1,2; Gao, Yuansheng1,2; Dou, Dou1,2
关键词Hypoxia Vasospasm Coronary circulation Contractility Myosin
刊名VASCULAR PHARMACOLOGY
2014-05-01
DOI10.1016/j.vph.2014.03.005
61期:2-3页:56-62
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
资助者National Natural Science Foundation of China ; Doctoral Fund of Ministry of Education for New Teachers ; Beijing Higher Education Young Elite Teacher Project ; National Natural Science Foundation of China ; Doctoral Fund of Ministry of Education for New Teachers ; Beijing Higher Education Young Elite Teacher Project
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]CA2+ CHANNELS ; SMOOTH-MUSCLE ; PULMONARY VASOCONSTRICTION ; DEPENDENT PHOSPHORYLATION ; MYOSIN PHOSPHATASE ; KINASE ACTIVATION ; PROTEIN-KINASE ; RHO-KINASE ; MECHANISM ; ANOXIA
英文摘要

Phosphoinositide 3-kinase (PI3K)/Akt signaling pathway plays an essential role in the regulation of vascular tone. The present study aimed to determine its role in hypoxic coronary vasoconstriction. Isometric tension of isolated porcine coronary arteries was measured with organ chamber technique; the protein levels of phosphorylated and total MLC were examined by Western blotting; the activities of PI3K and Rho kinase were determined by the phosphorylation of their respective target protein Akt and MTPT1. Acute hypoxia induced a rapid contraction followed by a short-term relaxation and then a sustained contraction in porcine coronary arteries. The rapid but not the sustained contraction was abolished by endothelium removal. The sustained contraction was attenuated by inhibitors of PI3K (LY294002) and Akt (Akt-I). The attenuation effect caused by LY294002 was not affected by nifedipine, but was abolished by Y27632, an inhibitor of Rho kinase. The sustained hypoxic contraction was associated with altered phosphorylation of MLC and Akt, which was inhibited by LY294002. The sustained hypoxic contraction was also accompanied with increased phosphorylation of MYPT1, which was inhibited by LY294002 and Y27632. This study demonstrates that sustained hypoxia causes porcine coronary artery to contract in an endothelium-independent manner. An increased PI3K/Akt/Rho kinase signaling may be involved. (C) 2014 Elsevier Inc. All rights reserved.

语种英语
所属项目编号81001433 ; 81270341 ; 81373404 ; 20100001120037 ; YETP0054
资助者National Natural Science Foundation of China ; Doctoral Fund of Ministry of Education for New Teachers ; Beijing Higher Education Young Elite Teacher Project ; National Natural Science Foundation of China ; Doctoral Fund of Ministry of Education for New Teachers ; Beijing Higher Education Young Elite Teacher Project
WOS记录号WOS:000336557600003
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64842
专题基础医学院_心血管所
作者单位1.Heze Med Coll, Dept Physiol, Heze, Shandong, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100191, Peoples R China
3.Peking Univ, Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Liu, Huixia,Chen, Zhengju,Liu, Juan,et al. Endothelium-independent hypoxic contraction of porcine coronary arteries may be mediated by activation of phosphoinositide 3-kinase/Akt pathway[J]. VASCULAR PHARMACOLOGY,2014,61(2-3):56-62.
APA Liu, Huixia,Chen, Zhengju,Liu, Juan,Liu, Limei,Gao, Yuansheng,&Dou, Dou.(2014).Endothelium-independent hypoxic contraction of porcine coronary arteries may be mediated by activation of phosphoinositide 3-kinase/Akt pathway.VASCULAR PHARMACOLOGY,61(2-3),56-62.
MLA Liu, Huixia,et al."Endothelium-independent hypoxic contraction of porcine coronary arteries may be mediated by activation of phosphoinositide 3-kinase/Akt pathway".VASCULAR PHARMACOLOGY 61.2-3(2014):56-62.
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