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学科主题: 临床医学
题名:
Assessment of a five-color flow cytometric assay for verifying automated white blood cell differentials
作者: Huang Chun-mei1,2; Yu Lian-hui1,2; Pu Cheng-wei3; Wang Xin1,2; Wang Geng1,2; Shen Li-song4; Wang Jian-zhong3; Cui Wei1,2
关键词: flow cytometry ; white blood cell count ; hematology ; manual microscopy
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2013-02-20
DOI: 10.3760/cma.j.issn.0366-6999.20122255
卷: 126, 期:4, 页:716-721
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: HEMATOLOGY ANALYZER ; FLUORESCENCE ; SAMPLES ; COUNT
英文摘要:

Background White blood cell (WBC) counts and differentials performed using an automated cell counter typically require manual microscopic review. However, this last step is time consuming and requires experienced personnel. We evaluated the clinical efficiency of using flow cytometry (FCM) employing a six-antibody/five-color reagent for verifying automated WBC differentials.

Methods A total of 56 apparently healthy samples were assessed using a five-color flow cytometer to verify the normal reference ranges of WBC differentials. WBC differentials of 622 samples were also determined using both a cell counter and FCM. These results were then confirmed using manual microscopic methods.

Results The probabilities for all of the parameters of WBC differentials exceeded the corresponding normal reference ranges by no more than 7.5%. The resulting WBC differentials were well correlated between FCM and the cell counter (r >0.88, P <0.001), except in the case of basophils. Neutrophils, lymphocytes, and eosinophils were well correlated between FCM and standard microscopic cytology assessment (r >0.80, P <0.001). The sensitivities of FCM for identification of immature granulocytes and blast cells (72.03% and 22.22%, respectively) were higher than those of the cell counter method (44.92% and 11.11%, respectively). The specificities of FCM were all above 85%, substantially better than those of the cell counter method.

Conclusion These five-color FCM assays could be applied to accurately verify abnormal results of automated assessment of WBC differentials.

语种: 英语
WOS记录号: WOS:000319793800021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64863
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: 1.Chinese Acad Med Sci, Dept Clin Lab, Beijing 100730, Peoples R China
2.Beijing Union Med Coll Hosp, Beijing 100730, Peoples R China
3.Peking Univ, Hosp 1, Dept Clin Lab, Beijing 100034, Peoples R China
4.Jiao Tong Univ, Sch Med, Xin Hua Hosp Affiliated, Dept Clin Lab, Shanghai 200092, Peoples R China

Recommended Citation:
Huang Chun-mei,Yu Lian-hui,Pu Cheng-wei,et al. Assessment of a five-color flow cytometric assay for verifying automated white blood cell differentials[J]. CHINESE MEDICAL JOURNAL,2013,126(4):716-721.
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