IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Telomerase activity and expression of the telomerase catalytic subunit gene in non-small cell lung cancer: correlation with decreased apoptosis and clinical prognosis
Wang, J; Liu, XY; Jiang, W; Liang, L
关键词telomerase activity telomerase catalytic subunit gene apoptosis bcl-2 non-small cell lung cancer
刊名CHINESE MEDICAL JOURNAL
2000-11-01
113期:11页:985-990
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
英文摘要

Objective To investigate the level of telomerase activation (TA) and telomerase catalytic subunit (hEST(2)) gene mRNA in patients with non-small cell lung cancer, and determine whether they are associated with tumor cell apoptosis, stage, clinical outcome.

Methods Primary tumor specimens from 58 patients untreated with chemotherapy and 10 cases of histologically benign and adjacent lung tissue were analyzed. TA and hEST2 were measured by means of a modified telomerase repeat amplification protocol (TRAP) assay and in situ hybridization (ISH), respectively. Terminal deoxynucleotidyl transferase ( TdT)-mediated biotin dUTP nick end-labeling (TUNEL) was used to evaluate apoptotic cells. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect bcl-2 mRNA expression.

Results TA and hEST2 were detected in 45 (77.6%) and 43 (74.1%) of 58 tumor specimens, respectively, and not detected in specimens of adjacent and benign lung tissue. One case expressed hEST(2), as a weak positive. Statistically significant positive association was found between the level of TA and hEST(2) (r=0.85, P=0.001). TA and hEST(2) were associated with tumor stage, but not associated with tumor grade, gender and patient age. Positive rate of bcl-2 mRNA was 38 (65.5%) of 58 tumor specimens. The mean apoptotic index in the bcl-2 positive group (9.5+/-1.3) was lower than that in the bcl-2 negative one (19.8+/-2.1, P<0.05), suggesting that apoptotic index may be inversely associated with bcl-2 expression (r= - 0.48, P = 0.041). Bcl-2 expression in the TA and hEST(2) positive group (92.1% and 89.4%) was higher than that in the negative one (50.0% and 45.0%, P = 0.043 and P = 0.032, respectively). The apoptotic index was lower in the TA or hEST2 positive group (8.2 +/- 1.4, 10.7 +/- 1.1) then in the negative one (20.5 +/- 1.6, 24.2+/-2.1, P<0.05). A statistically significant inverse association was found between TA or hEST(2) and apoptotic index (r = - 0.45, P = 0.02 and r = -0.51, P=0.001, respectively). Positive correlation was also detected between TA or hEST2 and bcl-2 expression (r = 0.86, P = 0.01 and r = 0.73, P = 0.024, respectively). The level of hEST(2) mRNA and apoptotic index were associated with clinical outcome in a multivariate cox regression analysis.

Conclusions High TA and hEST(2) were frequently detected in primary non-small cell lung cancer untreated with chemotherapy, having high bcl-2 expression and a low tumor cell apoptotic rate. This suggests that both TA and hEST(2) are correlated with the deregulation of apoptosis. hEST(2) and apoptotic index have prognostic significance in patients with non-small cell lung cancer.

语种英语
WOS记录号WOS:000165752600006
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64870
专题北京大学临床肿瘤学院
作者单位Beijing Univ, Sch Oncol, Dept Internal Med, Beijing 100036, Peoples R China
推荐引用方式
GB/T 7714
Wang, J,Liu, XY,Jiang, W,et al. Telomerase activity and expression of the telomerase catalytic subunit gene in non-small cell lung cancer: correlation with decreased apoptosis and clinical prognosis[J]. CHINESE MEDICAL JOURNAL,2000,113(11):985-990.
APA Wang, J,Liu, XY,Jiang, W,&Liang, L.(2000).Telomerase activity and expression of the telomerase catalytic subunit gene in non-small cell lung cancer: correlation with decreased apoptosis and clinical prognosis.CHINESE MEDICAL JOURNAL,113(11),985-990.
MLA Wang, J,et al."Telomerase activity and expression of the telomerase catalytic subunit gene in non-small cell lung cancer: correlation with decreased apoptosis and clinical prognosis".CHINESE MEDICAL JOURNAL 113.11(2000):985-990.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, J]的文章
[Liu, XY]的文章
[Jiang, W]的文章
百度学术
百度学术中相似的文章
[Wang, J]的文章
[Liu, XY]的文章
[Jiang, W]的文章
必应学术
必应学术中相似的文章
[Wang, J]的文章
[Liu, XY]的文章
[Jiang, W]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。