|Telomerase activity and expression of the telomerase catalytic subunit gene in non-small cell lung cancer: correlation with decreased apoptosis and clinical prognosis|
|Wang, J; Liu, XY; Jiang, W; Liang, L|
|关键词||telomerase activity telomerase catalytic subunit gene apoptosis bcl-2 non-small cell lung cancer|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
Objective To investigate the level of telomerase activation (TA) and telomerase catalytic subunit (hEST(2)) gene mRNA in patients with non-small cell lung cancer, and determine whether they are associated with tumor cell apoptosis, stage, clinical outcome.
Methods Primary tumor specimens from 58 patients untreated with chemotherapy and 10 cases of histologically benign and adjacent lung tissue were analyzed. TA and hEST2 were measured by means of a modified telomerase repeat amplification protocol (TRAP) assay and in situ hybridization (ISH), respectively. Terminal deoxynucleotidyl transferase ( TdT)-mediated biotin dUTP nick end-labeling (TUNEL) was used to evaluate apoptotic cells. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect bcl-2 mRNA expression.
Results TA and hEST2 were detected in 45 (77.6%) and 43 (74.1%) of 58 tumor specimens, respectively, and not detected in specimens of adjacent and benign lung tissue. One case expressed hEST(2), as a weak positive. Statistically significant positive association was found between the level of TA and hEST(2) (r=0.85, P=0.001). TA and hEST(2) were associated with tumor stage, but not associated with tumor grade, gender and patient age. Positive rate of bcl-2 mRNA was 38 (65.5%) of 58 tumor specimens. The mean apoptotic index in the bcl-2 positive group (9.5+/-1.3) was lower than that in the bcl-2 negative one (19.8+/-2.1, P<0.05), suggesting that apoptotic index may be inversely associated with bcl-2 expression (r= - 0.48, P = 0.041). Bcl-2 expression in the TA and hEST(2) positive group (92.1% and 89.4%) was higher than that in the negative one (50.0% and 45.0%, P = 0.043 and P = 0.032, respectively). The apoptotic index was lower in the TA or hEST2 positive group (8.2 +/- 1.4, 10.7 +/- 1.1) then in the negative one (20.5 +/- 1.6, 24.2+/-2.1, P<0.05). A statistically significant inverse association was found between TA or hEST(2) and apoptotic index (r = - 0.45, P = 0.02 and r = -0.51, P=0.001, respectively). Positive correlation was also detected between TA or hEST2 and bcl-2 expression (r = 0.86, P = 0.01 and r = 0.73, P = 0.024, respectively). The level of hEST(2) mRNA and apoptotic index were associated with clinical outcome in a multivariate cox regression analysis.
Conclusions High TA and hEST(2) were frequently detected in primary non-small cell lung cancer untreated with chemotherapy, having high bcl-2 expression and a low tumor cell apoptotic rate. This suggests that both TA and hEST(2) are correlated with the deregulation of apoptosis. hEST(2) and apoptotic index have prognostic significance in patients with non-small cell lung cancer.
|作者单位||Beijing Univ, Sch Oncol, Dept Internal Med, Beijing 100036, Peoples R China|
|Wang, J,Liu, XY,Jiang, W,et al. Telomerase activity and expression of the telomerase catalytic subunit gene in non-small cell lung cancer: correlation with decreased apoptosis and clinical prognosis[J]. CHINESE MEDICAL JOURNAL,2000,113(11):985-990.|
|APA||Wang, J,Liu, XY,Jiang, W,&Liang, L.(2000).Telomerase activity and expression of the telomerase catalytic subunit gene in non-small cell lung cancer: correlation with decreased apoptosis and clinical prognosis.CHINESE MEDICAL JOURNAL,113(11),985-990.|
|MLA||Wang, J,et al."Telomerase activity and expression of the telomerase catalytic subunit gene in non-small cell lung cancer: correlation with decreased apoptosis and clinical prognosis".CHINESE MEDICAL JOURNAL 113.11(2000):985-990.|