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Ameliorated chrysotile-induced DNA damage in human embryo lung cells by surface modification of chrysotile with rare earth compounds
Fan, JG; Wang, QE; Liu, SJ
刊名BIOMEDICAL AND ENVIRONMENTAL SCIENCES
2001-09-01
14期:3页:220-228
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Environmental Sciences ; Public, Environmental & Occupational Health
研究领域[WOS]Environmental Sciences & Ecology ; Public, Environmental & Occupational Health
关键词[WOS]MESOTHELIAL CELLS ; CROCIDOLITE ASBESTOS ; MINERAL FIBERS ; CYTOTOXICITY ; CARCINOGENICITY ; INVITRO ; INVIVO ; LONG
英文摘要

Objective In view of the fact that asbestos is not only a key occupational hazard, but also an important environmental pollutant, it is necessary to develop a proper method to decrease the carcinogenecity of asbestos fibers. This study was designed to determine if the surface modification of chrysotile asbestos fiber (CAF) with rare earth compounds (REC) can ameliorate CAF-induced DNA damages in human embryo lung (HEL) cells. Methods After incubation with REC solution at different concentrations at mom temperature for 1 h, natural and REC-pretreated CAF was added to cell culture at various doses. At the selected time as the experiment designed, DNA damages of the HEL cells were detected by Unscheduled DNA Synthesis (UDS) and Single Cell Gel Electrophoresis (SCGE) assays. Results The UDS induced by natural CAF was elevated with the increase of CAF doses. There was a good dose-response relationship between the UDS and the amount of CAF in the medium and the coefficient of correlation (R) was 0.958 at P < 0.05. In REC-pretreated CAF groups, the UDS declined with the increase of REC doses. Both catalase (CAT) and dimethylsulfoxide (DMSO) also reduced the CAF-induced enhancement of UDS. In SCGE assay, CAF induced DNA chain breakage and the magnitude of DNA chain breakage increased in a dose-dependent manner and the coefficient of correlation (R) was 0.992 at p < 0.01, while REC-pretreated CAF significantly decreased the induction of DNA chain breakage in a dose-dependent manner(r = 0.989, P < 0.05). Conclusion It can be concluded that CAF-induced DNA damages in HEL cells may be partly mediated by oxygen derivatives, and the surface modification of CAF with REC might hide critical sites on the fiber surface, thereby reducing the fiber-mediated production of oxygen derivation and lowering the CAF-induced UDS and DNA chain breakage in HEL cells.

语种英语
WOS记录号WOS:000171205000007
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64880
Collection北京大学公共卫生学院
作者单位1.Natl Ctr Occupat Safety Sci & Technol, Beijing 100029, Peoples R China
2.Beijing Med Univ, Sch Publ Hlth, Dept Occupat Hlth, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Fan, JG,Wang, QE,Liu, SJ. Ameliorated chrysotile-induced DNA damage in human embryo lung cells by surface modification of chrysotile with rare earth compounds[J]. BIOMEDICAL AND ENVIRONMENTAL SCIENCES,2001,14(3):220-228.
APA Fan, JG,Wang, QE,&Liu, SJ.(2001).Ameliorated chrysotile-induced DNA damage in human embryo lung cells by surface modification of chrysotile with rare earth compounds.BIOMEDICAL AND ENVIRONMENTAL SCIENCES,14(3),220-228.
MLA Fan, JG,et al."Ameliorated chrysotile-induced DNA damage in human embryo lung cells by surface modification of chrysotile with rare earth compounds".BIOMEDICAL AND ENVIRONMENTAL SCIENCES 14.3(2001):220-228.
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