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学科主题: 口腔医学
题名:
Development of species-specific primers for detection of Streptococcus mutans in mixed bacterial samples
作者: Chen, Zhou; Saxena, Deepak; Caufield, Page W.; Ge, Yao; Wang, Minqi; Li, Yihong
关键词: Streptococcus mutans ; PCR ; species-specific primer ; dental caries
刊名: FEMS MICROBIOLOGY LETTERS
发表日期: 2007-07-01
DOI: 10.1111/j.1574-6968.2007.00756.x
卷: 272, 期:2, 页:154-162
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
关键词[WOS]: PHOSPHOENOLPYRUVATE PHOSPHOTRANSFERASE SYSTEM ; SURFACE PROTEIN ANTIGEN ; REAL-TIME PCR ; RAPID IDENTIFICATION ; ORAL STREPTOCOCCI ; RIBOSOMAL-RNA ; STRAINS ; HTRA ; SOBRINUS ; REGION
英文摘要:

Streptococcus mutans is the major microbial pathogen associated with dental caries in children. The objectives of this study were to design and evaluate species-specific primers for the identification of S. mutans. Validation of the best primer set, Sm479F/R, was performed using seven S. mutans reference strains, 48 ATCC non-S. mutans strains, 92 S. mutans clinical isolates, DNA samples of S. mutans-Streptococcus sobrinus or S. mutans-Streptococcus sanguinis, and mixed bacterial DNA of saliva samples from 33 18-month-old children. All of the S. mutans samples tested positive, and no PCR products were amplified from members of the other streptococci or nonstreptococci strains examined. The lowest detection level for PCR was 10(-2) ng of S. mutans DNA (c. 4.6 x 10(3) copies) in the test samples. The results of this study suggest that the Sm479F/R primer pair is highly specific and sensitive for identification of S. mutans in either purified or mixed DNA samples.

语种: 英语
WOS记录号: WOS:000247319500004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64881
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.NYU, Coll Dent, Dept Basic Sci & Craniofacial Biol, New York, NY 10010 USA
2.Peking Univ, Sch Stomatol, Beijing 100871, Peoples R China
3.Univ Maryland, Coll Hlth & Human Performance, College Pk, MD 20742 USA

Recommended Citation:
Chen, Zhou,Saxena, Deepak,Caufield, Page W.,et al. Development of species-specific primers for detection of Streptococcus mutans in mixed bacterial samples[J]. FEMS MICROBIOLOGY LETTERS,2007,272(2):154-162.
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