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Development of species-specific primers for detection of Streptococcus mutans in mixed bacterial samples
Chen, Zhou; Saxena, Deepak; Caufield, Page W.; Ge, Yao; Wang, Minqi; Li, Yihong
关键词Streptococcus mutans PCR species-specific primer dental caries
刊名FEMS MICROBIOLOGY LETTERS
2007-07-01
DOI10.1111/j.1574-6968.2007.00756.x
272期:2页:154-162
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Microbiology
研究领域[WOS]Microbiology
关键词[WOS]PHOSPHOENOLPYRUVATE PHOSPHOTRANSFERASE SYSTEM ; SURFACE PROTEIN ANTIGEN ; REAL-TIME PCR ; RAPID IDENTIFICATION ; ORAL STREPTOCOCCI ; RIBOSOMAL-RNA ; STRAINS ; HTRA ; SOBRINUS ; REGION
英文摘要

Streptococcus mutans is the major microbial pathogen associated with dental caries in children. The objectives of this study were to design and evaluate species-specific primers for the identification of S. mutans. Validation of the best primer set, Sm479F/R, was performed using seven S. mutans reference strains, 48 ATCC non-S. mutans strains, 92 S. mutans clinical isolates, DNA samples of S. mutans-Streptococcus sobrinus or S. mutans-Streptococcus sanguinis, and mixed bacterial DNA of saliva samples from 33 18-month-old children. All of the S. mutans samples tested positive, and no PCR products were amplified from members of the other streptococci or nonstreptococci strains examined. The lowest detection level for PCR was 10(-2) ng of S. mutans DNA (c. 4.6 x 10(3) copies) in the test samples. The results of this study suggest that the Sm479F/R primer pair is highly specific and sensitive for identification of S. mutans in either purified or mixed DNA samples.

语种英语
WOS记录号WOS:000247319500004
引用统计
被引频次:25[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64881
专题北京大学口腔医学院
作者单位1.NYU, Coll Dent, Dept Basic Sci & Craniofacial Biol, New York, NY 10010 USA
2.Peking Univ, Sch Stomatol, Beijing 100871, Peoples R China
3.Univ Maryland, Coll Hlth & Human Performance, College Pk, MD 20742 USA
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GB/T 7714
Chen, Zhou,Saxena, Deepak,Caufield, Page W.,et al. Development of species-specific primers for detection of Streptococcus mutans in mixed bacterial samples[J]. FEMS MICROBIOLOGY LETTERS,2007,272(2):154-162.
APA Chen, Zhou,Saxena, Deepak,Caufield, Page W.,Ge, Yao,Wang, Minqi,&Li, Yihong.(2007).Development of species-specific primers for detection of Streptococcus mutans in mixed bacterial samples.FEMS MICROBIOLOGY LETTERS,272(2),154-162.
MLA Chen, Zhou,et al."Development of species-specific primers for detection of Streptococcus mutans in mixed bacterial samples".FEMS MICROBIOLOGY LETTERS 272.2(2007):154-162.
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