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Biochanin A Induction of Sulfotransferases in Rats
Chen, Yue1; Huang, Chaoqun1; Zhou, Tianyan2; Zhang, Shunfen1; Chen, Guangping1
关键词Biochanin A Rat sulfotransferase Induction Gene regulation
刊名JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
2010
DOI10.1002/jbt.20318
24期:2页:102-114
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Toxicology
研究领域[WOS]Biochemistry & Molecular Biology ; Toxicology
关键词[WOS]ARYL-HYDROCARBON RECEPTOR ; MESSENGER-RNA EXPRESSION ; HYDROXYSTEROID SULFOTRANSFERASE ; GENE-EXPRESSION ; LIVER ; CELLS ; PHARMACOKINETICS ; BIOAVAILABILITY ; ISOFLAVONES ; ACTIVATION
英文摘要

Biochanin A (BCA) is a dietary isoflavone present in red clover (Trifoliumn pretense) and many herbal products. BCA has been reported to have chemopreventive actions against various cancers including prostate, breast, colon cancer, and so on. Sulfotransferases are a family of phase II drug-metabolizing enzymes, which are important for xenobiotic detoxification and regulation of biological signaling molecule biological activities. Sulfotransferase gene expressions are regulated by different hormones and xenobiotics. Improper regulation of sulfotransferases leads to improper functions of biological signaling molecules, which in turn can cause cancer or other diseases. BCA inhibits the enzyme activities of the phase I drug-metabolizing enzymes CYP1A1 and CYP1B1 in Chinese hamster ovary cells and induces the phase II drug-metabolizing enzymes UDP-glucuronosyltransferases in human prostate cancer cells. BCA induction of sulfotransferases has not been studied. This investigation evaluates the in vivo regulation of sulfotransferases at protein and mRNA levels in the liver and intestine of Sprague-Dawley rats treated with BCA (0, 2, 10, and 50 mg/kg/day) for 7 days. Our experimental results demonstrate for the first time that chronic BCA treatment can significantly induce the expression of rat sulfotransferase 1A1 (rSULT1A1, AST-IV), sulfotransferase 2A1 (rSULT2A1, STa), and rat estrogen sulfotransferase (rSULT1E1, EST) in rat liver and intestine. Our Western blot results are in good agreement with real-time RT-PCR data, suggesting that BCA induction of sulfotransferases occurs at the transcriptional level. (C) 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 24:102-114, 2010; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10:1002/jbt.20318

语种英语
WOS记录号WOS:000277134900004
项目编号GM078606 ; RSG-07-028-01-CNE ; 2006-35200-17137 ; HR05-015
资助机构NIH ; American Cancer Society ; USDA ; Oklahoma Center for the Advancement of Science and Technology (OCAST)
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64886
专题北京大学药学院_药剂学系
作者单位1.Oklahoma State Univ, Dept Physiol Sci, Ctr Vet Hlth Sci, Stillwater, OK 74078 USA
2.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Pharmaceut, Beijing 100083, Peoples R China
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GB/T 7714
Chen, Yue,Huang, Chaoqun,Zhou, Tianyan,et al. Biochanin A Induction of Sulfotransferases in Rats[J]. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY,2010,24(2):102-114.
APA Chen, Yue,Huang, Chaoqun,Zhou, Tianyan,Zhang, Shunfen,&Chen, Guangping.(2010).Biochanin A Induction of Sulfotransferases in Rats.JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY,24(2),102-114.
MLA Chen, Yue,et al."Biochanin A Induction of Sulfotransferases in Rats".JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY 24.2(2010):102-114.
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