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学科主题: 临床医学
题名:
Inhibition of neovascularization and expression shift of pro-/anti-angiogenic vascular endothelial growth factor isoforms after intravitreal bevacizumab injection in oxygen-induced-retinopathy mouse model
作者: Shi Xuan1; Zhao Min2,3; Xie Wan-kun2,3,6; Liang Jian-hong1; Miao Yi-fei4,5; Du Wei2,3; Li Xiao-xin1,3
关键词: bevacizumab ; vascular endothelial growth factor ; retinopathy of prematurity ; neovascularization
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2013-01-20
DOI: 10.3760/cma.j.issn.0366-6999.20122425
卷: 126, 期:2, 页:345-352
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: ANTI-VEGF AGENTS ; DIABETIC-RETINOPATHY ; SPLICE VARIANT ; CORNEAL NEOVASCULARIZATION ; RANDOMIZED-TRIAL ; VEGF(165)B ; PREMATURITY ; MICE ; CARCINOMA ; THERAPY
英文摘要:

Background Retinopathy of prematurity (ROP) has become one of the leading causes of visual loss in children. Vascular endothelial growth factor A (VEGF-A) is the principal stimulator of angiogenesis. VEGF was differentially spliced from exon 8 to exons 8a and 8b to form two families: the pro-angiogenic VEGFxxx family and the anti-angiogenic VEGFxxxb family. Previous research has shown variable effeteness of bevacizumab in inhibiting retinal neovascularization in ROP. This study aimed to investigate whether the effectiveness of this inhibition depends on the relative ratio of the two VEGF isoforms.

Methods Intravitreal bevacizumab injection (IVB) was performed in the oxygen-induced-retinopathy (OIR) mice on postnatal day 12 (P12) (intravitreal phosphate buffered saline (PBS) injection as control). The Evans blue perfused retina were used to test the retinal neovascularization-leakage (NVL) area and non-perfusion (NP) area.

Results The retinal NVL and NP area in the IVB group were significantly smaller than the intravitreal PBS injection group (IVP group). On P17, the protein level of total VEGF isoforms was significantly inhibited compared to IVP group (P<0.05) while VEGF(165)b isoform was slight reduced (P>0.05). The switch from pro-angiogenic isoforms to anti-angiogenic isoforms after IVB could be found. The relative protein expression of VEGF(165)b isoform was significantly higher in IVB group than in IVP group (P <0.05) on P17 which was correlated with the reduced ischemia-induced angiogenesis in OIR mice after IVB.

Conclusions The anti-angiogenic effectiveness might depend on the relative high expression of VEGF(165)b after intravitreal bevacizumab injection. Anti-angiogenic therapy is a more effective therapy for ROP.

语种: 英语
WOS记录号: WOS:000319632800026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64907
Appears in Collections:北京大学第二临床医学院_眼科_期刊论文

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作者单位: 1.Peking Univ, Dept Ophthalmol, Peoples Hosp, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Eye Inst, Peoples Hosp, Beijing 100044, Peoples R China
3.Minist Educ, Key Lab Vis Loss & Restorat, Beijing 100044, Peoples R China
4.Peking Univ, Dept Physiol & Pathophysiol, Hlth Sci Ctr, Beijing 100083, Peoples R China
5.Minist Educ, Key Lab Cardiovasc Sci, Beijing 100083, Peoples R China
6.China Acad, Chinese Med Sci Eye Hosp, Beijing 100040, Peoples R China

Recommended Citation:
Shi Xuan,Zhao Min,Xie Wan-kun,et al. Inhibition of neovascularization and expression shift of pro-/anti-angiogenic vascular endothelial growth factor isoforms after intravitreal bevacizumab injection in oxygen-induced-retinopathy mouse model[J]. CHINESE MEDICAL JOURNAL,2013,126(2):345-352.
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