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学科主题基础医学
Characterization of a hypertriglyceridemic transgenic miniature pig model expressing human apolipoprotein CIII
Wei, Jingyuan2,3,4,5; Ouyang, Hongsheng3,6; Wang, Yuhui1; Pang, Daxin3,6; Cong, Nathan X.1; Wang, Tiedong3,6; Leng, Bingfeng3,6; Li, Dong3,6; Li, Xiaoping3,6; Wu, Rong3,6; Ding, Yu2; Gao, Fei3,6; Deng, Yanhong6; Liu, Bo6; Li, Ziyi3,6; Lai, Liangxue3,6; Feng, Haihua3,6,7; Liu, George1; Deng, Xuming3,6,7
关键词apolipoprotein CIII hypertriglyceridemia miniature pig model transgenic
刊名FEBS JOURNAL
2012
DOI10.1111/j.1742-4658.2011.08401.x
279期:1页:91-99
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]APOA-I GENE ; INTESTINAL EXPRESSION ; TRIGLYCERIDE LEVELS ; APOCIII ENHANCER ; MICE ; ATHEROSCLEROSIS ; PLASMA ; DISEASE ; EVENTS ; LEVEL
英文摘要

Hypertriglyceridemia has recently been considered to be an independent risk factor for coronary heart disease, in which apolipoprotein (Apo)CIII is one of the major contributory factors, as it is strongly correlated with plasma triglyceride levels. Although ApoCIII transgenic mice have been generated as an animal model for the study of hypertriglyceridemia, the features of lipoprotein metabolism in mice differ greatly from those in humans. Because of the great similarity between pigs and humans with respect to lipid metabolism and cardiovascular physiology, we generated transgenic miniature pigs expressing human ApoCIII by the transfection of somatic cells combined with nuclear transfer. The expression of human ApoCIII was detected in the liver and intestine of the transgenic pigs. As compared with nontransgenic controls, transgenic pigs showed significantly increased plasma triglyceride levels (83 +/- 36 versus 38 +/- 4 mg.dL(-1), P < 0.01) when fed a chow diet. Plasma lipoprotein profiling by FPLC in transgenic animals showed a higher peak in large-particle fractions corresponding to very low-density lipoprotein/chylomicrons when triglyceride content in the fractions was assayed. There was not much difference in cholesterol content in FPLC fractions, although a large low-density lipoprotein peak was identified in both nontransgenic and transgenic animals, resembling that found in humans. Further analysis revealed markedly delayed clearance of plasma triglyceride, accompanied by significantly reduced lipoprotein lipase activity in post-heparin plasma, in transgenic pigs as compared with nontransgenic controls. In summary, we have successfully generated a novel hypertriglyceridemic ApoCIII transgenic miniature pig model that could be of great value for studies on hyperlipidemia in relation to atherosclerotic disorders.

语种英语
WOS记录号WOS:000298063400010
项目编号31172172 ; 30821001 ; 2011CB503900 ; 5101004
资助机构National Natural Science Foundation of China ; Major National Basic Research Program of the People&prime ; s Republic of China ; Beijing Natural Science Foundation
引用统计
被引频次:28[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64923
专题北京大学基础医学院_心血管所
北京大学第一临床医学院_病理科
北京大学口腔医学院_牙周科
作者单位1.Ctr Anim Embryo Engn Jilin Prov, Changchun, Peoples R China
2.Jilin Univ, Inst Zoonesis, Changchun 130062, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Inst Cardiovasc Sci, Beijing 100191, Peoples R China
4.Jilin Univ, Coll Anim Sci & Vet Med, Changchun 130062, Peoples R China
5.Jilin Univ, Lab Anim Ctr, Changchun 130062, Peoples R China
6.Jilin Prov Key Lab Anim Embryo Engn, Changchun, Peoples R China
7.Liaoning Prov Acad Analyt Sci, Shenyang, Peoples R China
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Wei, Jingyuan,Ouyang, Hongsheng,Wang, Yuhui,et al. Characterization of a hypertriglyceridemic transgenic miniature pig model expressing human apolipoprotein CIII[J]. FEBS JOURNAL,2012,279(1):91-99.
APA Wei, Jingyuan.,Ouyang, Hongsheng.,Wang, Yuhui.,Pang, Daxin.,Cong, Nathan X..,...&Deng, Xuming.(2012).Characterization of a hypertriglyceridemic transgenic miniature pig model expressing human apolipoprotein CIII.FEBS JOURNAL,279(1),91-99.
MLA Wei, Jingyuan,et al."Characterization of a hypertriglyceridemic transgenic miniature pig model expressing human apolipoprotein CIII".FEBS JOURNAL 279.1(2012):91-99.
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