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学科主题: 基础医学
题名:
Characterization of a hypertriglyceridemic transgenic miniature pig model expressing human apolipoprotein CIII
作者: Wei, Jingyuan2,3,4,5; Ouyang, Hongsheng3,6; Wang, Yuhui1; Pang, Daxin3,6; Cong, Nathan X.1; Wang, Tiedong3,6; Leng, Bingfeng3,6; Li, Dong3,6; Li, Xiaoping3,6; Wu, Rong3,6; Ding, Yu2; Gao, Fei3,6; Deng, Yanhong6; Liu, Bo6; Li, Ziyi3,6; Lai, Liangxue3,6; Feng, Haihua3,6,7; Liu, George1; Deng, Xuming3,6,7
关键词: apolipoprotein CIII ; hypertriglyceridemia ; miniature pig ; model ; transgenic
刊名: FEBS JOURNAL
发表日期: 2012
DOI: 10.1111/j.1742-4658.2011.08401.x
卷: 279, 期:1, 页:91-99
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: APOA-I GENE ; INTESTINAL EXPRESSION ; TRIGLYCERIDE LEVELS ; APOCIII ENHANCER ; MICE ; ATHEROSCLEROSIS ; PLASMA ; DISEASE ; EVENTS ; LEVEL
英文摘要:

Hypertriglyceridemia has recently been considered to be an independent risk factor for coronary heart disease, in which apolipoprotein (Apo)CIII is one of the major contributory factors, as it is strongly correlated with plasma triglyceride levels. Although ApoCIII transgenic mice have been generated as an animal model for the study of hypertriglyceridemia, the features of lipoprotein metabolism in mice differ greatly from those in humans. Because of the great similarity between pigs and humans with respect to lipid metabolism and cardiovascular physiology, we generated transgenic miniature pigs expressing human ApoCIII by the transfection of somatic cells combined with nuclear transfer. The expression of human ApoCIII was detected in the liver and intestine of the transgenic pigs. As compared with nontransgenic controls, transgenic pigs showed significantly increased plasma triglyceride levels (83 +/- 36 versus 38 +/- 4 mg.dL(-1), P < 0.01) when fed a chow diet. Plasma lipoprotein profiling by FPLC in transgenic animals showed a higher peak in large-particle fractions corresponding to very low-density lipoprotein/chylomicrons when triglyceride content in the fractions was assayed. There was not much difference in cholesterol content in FPLC fractions, although a large low-density lipoprotein peak was identified in both nontransgenic and transgenic animals, resembling that found in humans. Further analysis revealed markedly delayed clearance of plasma triglyceride, accompanied by significantly reduced lipoprotein lipase activity in post-heparin plasma, in transgenic pigs as compared with nontransgenic controls. In summary, we have successfully generated a novel hypertriglyceridemic ApoCIII transgenic miniature pig model that could be of great value for studies on hyperlipidemia in relation to atherosclerotic disorders.

语种: 英语
所属项目编号: 31172172 ; 30821001 ; 2011CB503900 ; 5101004
项目资助者: National Natural Science Foundation of China ; Major National Basic Research Program of the People&prime ; s Republic of China ; Beijing Natural Science Foundation
WOS记录号: WOS:000298063400010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64923
Appears in Collections:基础医学院_心血管所_期刊论文

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作者单位: 1.Ctr Anim Embryo Engn Jilin Prov, Changchun, Peoples R China
2.Jilin Univ, Inst Zoonesis, Changchun 130062, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Inst Cardiovasc Sci, Beijing 100191, Peoples R China
4.Jilin Univ, Coll Anim Sci & Vet Med, Changchun 130062, Peoples R China
5.Jilin Univ, Lab Anim Ctr, Changchun 130062, Peoples R China
6.Jilin Prov Key Lab Anim Embryo Engn, Changchun, Peoples R China
7.Liaoning Prov Acad Analyt Sci, Shenyang, Peoples R China

Recommended Citation:
Wei, Jingyuan,Ouyang, Hongsheng,Wang, Yuhui,et al. Characterization of a hypertriglyceridemic transgenic miniature pig model expressing human apolipoprotein CIII[J]. FEBS JOURNAL,2012,279(1):91-99.
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