IR@PKUHSC  > 北京大学第二临床医学院
学科主题临床医学
A human endothelial cell feeder system that efficiently supports the undifferentiated growth of mouse embryonic stem cells
Zhou, Haisheng1,2; Yong, Jun1,2; Sun, Xiaomeng1,2; Wang, Chengyan1,2; Yang, Weifeng1,2; Zhang, Pengbo1,2; Zhu, Jingliang3; Shi, Cheng4; Ding, Mingxiao1,2; Deng, Hongkui1,2,5
关键词mouse embryonic stem cells human endothelial cells cell proliferation
刊名DIFFERENTIATION
2008-11-01
DOI10.1111/j.1432-0436.2008.00280.x
76期:9页:923-930
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Developmental Biology
研究领域[WOS]Cell Biology ; Developmental Biology
关键词[WOS]IL-6 SIGNAL TRANSDUCER ; IN-VITRO ; GENE-EXPRESSION ; SELF-RENEWAL ; YOLK-SAC ; RECEPTOR ; DIFFERENTIATION ; GP130 ; DERIVATION ; EXPANSION
英文摘要

Feeder cells are commonly used to culture embryonic stem cells to maintain their undifferentiated and pluripotent status. Conventionally, mouse embryonic fibroblasts (MEFs), supplemented with leukemia inhibitory factor (LIF), are used as feeder cells to support the growth of mouse embryonic stem cells (mESCs) in culture. To prepare for fresh MEF feeder or for MEF-conditioned medium, sacrifice of mouse fetuses repeatedly is unavoidable in these tedious culture systems. Here we report the discovery of a human endothelial cell line (ECV-304 cell line) that efficiently supports growth of mESCs LIF-free conditions. mESCs that were successfully cultured for eight to 20 passages on ECV-304 feeders showed morphological characteristics similar to cells cultured in traditional feeder cell systems. These cells expressed the stem cell markers Oct3/4, Nanog, Sox2, and SSEA-1. Furthermore, cells cultured on the ECV-304 cell line were able to differentiate into three germ layers and were able to generate chimeric mice. Compared with traditional culture systems, there is no requirement for mouse fetuses and exogenous LIF does not need to be added to the culture system. As a stable cell line, the ECV-304 cell line efficiently replaces MEFs as an effective feeder system and allows the efficient expansion of mESCs.

语种英语
WOS记录号WOS:000260254600001
项目编号NKBRP: 2005CB522504 ; 5051003 ; 20060390365
资助机构Science Fund for Creative Research Groups ; National Key Basic Research Program ; Natural Science Foundation of Beijing ; China Postdoctoral Science Foundation
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64929
专题北京大学第二临床医学院
北京大学第二临床医学院_妇科
作者单位1.Beijing Vitalriver Lab Anim Inc, Beijing, Peoples R China
2.Peking Univ, Peoples Hosp, Beijing 100871, Peoples R China
3.Peking Univ, Key Lab Cell Proliferat, Beijing 100871, Peoples R China
4.Peking Univ, Coll Life Sci, Differentiat Minist Educ, Beijing 100871, Peoples R China
5.Peking Univ, Shenzhen Grad Sch, Lab Chem Genom, Shenzhen, Peoples R China
推荐引用方式
GB/T 7714
Zhou, Haisheng,Yong, Jun,Sun, Xiaomeng,et al. A human endothelial cell feeder system that efficiently supports the undifferentiated growth of mouse embryonic stem cells[J]. DIFFERENTIATION,2008,76(9):923-930.
APA Zhou, Haisheng.,Yong, Jun.,Sun, Xiaomeng.,Wang, Chengyan.,Yang, Weifeng.,...&Deng, Hongkui.(2008).A human endothelial cell feeder system that efficiently supports the undifferentiated growth of mouse embryonic stem cells.DIFFERENTIATION,76(9),923-930.
MLA Zhou, Haisheng,et al."A human endothelial cell feeder system that efficiently supports the undifferentiated growth of mouse embryonic stem cells".DIFFERENTIATION 76.9(2008):923-930.
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