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学科主题: 临床医学
题名:
A human endothelial cell feeder system that efficiently supports the undifferentiated growth of mouse embryonic stem cells
作者: Zhou, Haisheng1,2; Yong, Jun1,2; Sun, Xiaomeng1,2; Wang, Chengyan1,2; Yang, Weifeng1,2; Zhang, Pengbo1,2; Zhu, Jingliang3; Shi, Cheng4; Ding, Mingxiao1,2; Deng, Hongkui1,2,5
关键词: mouse embryonic stem cells ; human endothelial cells ; cell proliferation
刊名: DIFFERENTIATION
发表日期: 2008-11-01
DOI: 10.1111/j.1432-0436.2008.00280.x
卷: 76, 期:9, 页:923-930
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Developmental Biology
研究领域[WOS]: Cell Biology ; Developmental Biology
关键词[WOS]: IL-6 SIGNAL TRANSDUCER ; IN-VITRO ; GENE-EXPRESSION ; SELF-RENEWAL ; YOLK-SAC ; RECEPTOR ; DIFFERENTIATION ; GP130 ; DERIVATION ; EXPANSION
英文摘要:

Feeder cells are commonly used to culture embryonic stem cells to maintain their undifferentiated and pluripotent status. Conventionally, mouse embryonic fibroblasts (MEFs), supplemented with leukemia inhibitory factor (LIF), are used as feeder cells to support the growth of mouse embryonic stem cells (mESCs) in culture. To prepare for fresh MEF feeder or for MEF-conditioned medium, sacrifice of mouse fetuses repeatedly is unavoidable in these tedious culture systems. Here we report the discovery of a human endothelial cell line (ECV-304 cell line) that efficiently supports growth of mESCs LIF-free conditions. mESCs that were successfully cultured for eight to 20 passages on ECV-304 feeders showed morphological characteristics similar to cells cultured in traditional feeder cell systems. These cells expressed the stem cell markers Oct3/4, Nanog, Sox2, and SSEA-1. Furthermore, cells cultured on the ECV-304 cell line were able to differentiate into three germ layers and were able to generate chimeric mice. Compared with traditional culture systems, there is no requirement for mouse fetuses and exogenous LIF does not need to be added to the culture system. As a stable cell line, the ECV-304 cell line efficiently replaces MEFs as an effective feeder system and allows the efficient expansion of mESCs.

语种: 英语
所属项目编号: NKBRP: 2005CB522504 ; 5051003 ; 20060390365
项目资助者: Science Fund for Creative Research Groups ; National Key Basic Research Program ; Natural Science Foundation of Beijing ; China Postdoctoral Science Foundation
WOS记录号: WOS:000260254600001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64929
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Beijing Vitalriver Lab Anim Inc, Beijing, Peoples R China
2.Peking Univ, Peoples Hosp, Beijing 100871, Peoples R China
3.Peking Univ, Key Lab Cell Proliferat, Beijing 100871, Peoples R China
4.Peking Univ, Coll Life Sci, Differentiat Minist Educ, Beijing 100871, Peoples R China
5.Peking Univ, Shenzhen Grad Sch, Lab Chem Genom, Shenzhen, Peoples R China

Recommended Citation:
Zhou, Haisheng,Yong, Jun,Sun, Xiaomeng,et al. A human endothelial cell feeder system that efficiently supports the undifferentiated growth of mouse embryonic stem cells[J]. DIFFERENTIATION,2008,76(9):923-930.
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