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学科主题: 口腔医学
题名:
Immunohistochemical localization of Pax6 in the developing tooth germ of mice
作者: Lei, Haihua1,2; Liu, He2; Ding, Yun1; Ge, Lihong2
关键词: Pax6 expression ; Odontogenesis ; Immunohistochemistry ; Mice
刊名: JOURNAL OF MOLECULAR HISTOLOGY
发表日期: 2014-08-01
DOI: 10.1007/s10735-014-9564-5
卷: 45, 期:4, 页:373-379
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: CRANIOFACIAL ABNORMALITIES ; OSTEOBLAST DIFFERENTIATION ; MOUSE ; TEETH ; GENE ; SUPERNUMERARY ; MUTATION ; CBFA1 ; OLIGODONTIA ; EXPRESSION
英文摘要:

Recent studies have reported that supernumerary teeth were observed in the maxillary incisor area in several Pax6 homozygous mutant mouse and rat strains. To date, it remains unknown whether Pax6 is expressed during tooth development in any species. The study aimed to analyze the expression of Pax6 during mouse incisor and molar development. C57BL/6J mouse embryos on days E12.5, E13.5, E14.5, E16.5 and E18.5 were produced. Heads from these embryos, as well as from P1.5 mice, were processed for paraffin wax embedding (N a parts per thousand yen 3 for each stage) and prepared for immunohistochemistry. Pax6 immunostaining was found in all tooth germs examined. At the E12.5 dental placode, E13.5 bud stage, E14.5 cap stage and E16.5 early bell stage, Pax6 was expressed in ectodermally derived tissues of tooth germs and oral epithelia adjacent to the tooth germs. Cells in the underlying dental ectomesenchyme that showed Pax9 expression were Pax6 negative. At E18.5 and P1.5, Pax6 was expressed in more differentiated ameloblasts and cells of the stratum intermedium and stellate reticulum that were derived from the oral epithelium, as well as in mesenchyme-derived differentiated odontoblasts. Pax6 expression was also observed in the submandibular gland, tongue filiform papilla and hair follicle at E16.5 and P1.5. The present study demonstrated that Pax6 was expressed in incisor and molar germs during mouse tooth development. The results provide a basis for exploring the function of Pax6 during tooth development.

语种: 英语
所属项目编号: 81170928
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000338284700002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64933
Appears in Collections:北京大学口腔医学院_儿童口腔科_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dent Ctr 3, Beijing 100083, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Dept Pediat Dent, Beijing 100081, Peoples R China

Recommended Citation:
Lei, Haihua,Liu, He,Ding, Yun,et al. Immunohistochemical localization of Pax6 in the developing tooth germ of mice[J]. JOURNAL OF MOLECULAR HISTOLOGY,2014,45(4):373-379.
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