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学科主题口腔医学
Immunohistochemical localization of Pax6 in the developing tooth germ of mice
Lei, Haihua1,2; Liu, He2; Ding, Yun1; Ge, Lihong2
关键词Pax6 expression Odontogenesis Immunohistochemistry Mice
刊名JOURNAL OF MOLECULAR HISTOLOGY
2014-08-01
DOI10.1007/s10735-014-9564-5
45期:4页:373-379
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]CRANIOFACIAL ABNORMALITIES ; OSTEOBLAST DIFFERENTIATION ; MOUSE ; TEETH ; GENE ; SUPERNUMERARY ; MUTATION ; CBFA1 ; OLIGODONTIA ; EXPRESSION
英文摘要

Recent studies have reported that supernumerary teeth were observed in the maxillary incisor area in several Pax6 homozygous mutant mouse and rat strains. To date, it remains unknown whether Pax6 is expressed during tooth development in any species. The study aimed to analyze the expression of Pax6 during mouse incisor and molar development. C57BL/6J mouse embryos on days E12.5, E13.5, E14.5, E16.5 and E18.5 were produced. Heads from these embryos, as well as from P1.5 mice, were processed for paraffin wax embedding (N a parts per thousand yen 3 for each stage) and prepared for immunohistochemistry. Pax6 immunostaining was found in all tooth germs examined. At the E12.5 dental placode, E13.5 bud stage, E14.5 cap stage and E16.5 early bell stage, Pax6 was expressed in ectodermally derived tissues of tooth germs and oral epithelia adjacent to the tooth germs. Cells in the underlying dental ectomesenchyme that showed Pax9 expression were Pax6 negative. At E18.5 and P1.5, Pax6 was expressed in more differentiated ameloblasts and cells of the stratum intermedium and stellate reticulum that were derived from the oral epithelium, as well as in mesenchyme-derived differentiated odontoblasts. Pax6 expression was also observed in the submandibular gland, tongue filiform papilla and hair follicle at E16.5 and P1.5. The present study demonstrated that Pax6 was expressed in incisor and molar germs during mouse tooth development. The results provide a basis for exploring the function of Pax6 during tooth development.

语种英语
WOS记录号WOS:000338284700002
项目编号81170928
资助机构National Natural Science Foundation of China
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64933
专题北京大学口腔医学院_儿童口腔科
北京大学口腔医学院_第三门诊部
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dent Ctr 3, Beijing 100083, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Dept Pediat Dent, `s\vl VE`s\vl VChina
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GB/T 7714
Lei, Haihua,Liu, He,Ding, Yun,et al. Immunohistochemical localization of Pax6 in the developing tooth germ of mice[J]. JOURNAL OF MOLECULAR HISTOLOGY,2014,45(4):373-379.
APA Lei, Haihua,Liu, He,Ding, Yun,&Ge, Lihong.(2014).Immunohistochemical localization of Pax6 in the developing tooth germ of mice.JOURNAL OF MOLECULAR HISTOLOGY,45(4),373-379.
MLA Lei, Haihua,et al."Immunohistochemical localization of Pax6 in the developing tooth germ of mice".JOURNAL OF MOLECULAR HISTOLOGY 45.4(2014):373-379.
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