IR@PKUHSC  > 北京大学第三临床医学院  > 消化科
学科主题临床医学
Microwave antigen retrieval blocks endogenous peroxidase activity in imunohistochemistry
Gao, Chun; Wang, Ai-Ying; Han, Yaing
关键词immunohistochemistry microwave antigen retrieval endogenous peroxidase citric acid paraffin-embedded sections
刊名APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY
2008-07-01
16期:4页:393-399
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Anatomy & Morphology ; Medical Laboratory Technology ; Pathology
研究领域[WOS]Anatomy & Morphology ; Medical Laboratory Technology ; Pathology
关键词[WOS]PARAFFIN-EMBEDDED TISSUES ; IMMUNOHISTOCHEMISTRY ; SECTIONS ; PH
英文摘要

Endogenous peroxidase activity blocking is one of the most critical steps for a successful immunostaining. A traditional procedure for this purpose was to use diluted hydrogen peroxide (H2O2) solution to gain a better visualization. Our current study was designed to test if the conventional antigen retrieval (AR) step with microwave oven could also inhibit endogenous peroxidase. Tissue sections were treated in following different ways before incubating with 3, 3′ diaminobenzidine tetrahydrochloride reaction mixture: (1) microwave alone, (2) 0.3% or 3% of H2O2 before AR, or (3) 0.3% or 3% of H2O2 after AR. Surprisingly, we found that all different treatments completely eliminated the endogenous enzymatic activity, mostly in eosinophils and erythrocytes, suggesting that microwave exposure itself is able to block the endogenous peroxidase. The results were validated in most gastrointestinal tract mucosa from human and some tissues from mice, including blood enriched tissues, such as liver and spleen., We have obtained perfect immunostaining without any H2O2 blocking procedure. On the basis of our observation, we conclude that blockage of endogenous peroxidase with H2O2 in immunostaining could be omitted and replaced by microwave oven heating.

语种英语
WOS记录号WOS:000257270100014
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64941
Collection北京大学第三临床医学院_消化科
作者单位Peking Univ, Hosp 3, Dept Gastroenterol, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Gao, Chun,Wang, Ai-Ying,Han, Yaing. Microwave antigen retrieval blocks endogenous peroxidase activity in imunohistochemistry[J]. APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY,2008,16(4):393-399.
APA Gao, Chun,Wang, Ai-Ying,&Han, Yaing.(2008).Microwave antigen retrieval blocks endogenous peroxidase activity in imunohistochemistry.APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY,16(4),393-399.
MLA Gao, Chun,et al."Microwave antigen retrieval blocks endogenous peroxidase activity in imunohistochemistry".APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY 16.4(2008):393-399.
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