IR@PKUHSC  > 北京大学基础医学院  > 细胞生物学系
学科主题基础医学
Proteome analysis of hepatocellular carcinoma by two-dimensional difference gel electrophoresis
Sun, Wei; Xing, Baocai; Sun, Yi; Du, Xiaojuan; Lu, Min; Hao, Chunyi; Lu, Zhuang; Mi, Wei; Wu, Songfeng; Wei, Handong; Gao, Xue; Zhu, Yunping; Jiang, Ying; Qian, Xiaohong; He, Fuchu
刊名MOLECULAR & CELLULAR PROTEOMICS
2007-10-01
DOI10.1074/mcp.M600449-MCP200
6期:10页:1798-1808
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEINS ; HEAT-SHOCK PROTEINS ; MASS-SPECTROMETRY ; GENE-EXPRESSION ; MESSENGER-RNA ; HEPATITIS-C ; CELL-LINE ; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE ; BIOENERGETIC SIGNATURE ; LIVER-CIRRHOSIS
英文摘要

Hepatocellular carcinoma (HCC) is a highly malignant tumor, and chronic infection with hepatitis B virus is one of its major risk factors. To identify the proteins involved in HCC carcinogenesis, we used two-dimensional fluorescence DIGE to study the differentially expressed proteins in tumor and adjacent nontumor tissue samples. Samples from 12 hepatitis B virus-associated HCC patients were analyzed. A total of 61 spots were significantly up-regulated ( ratio >= 2, p <= 0.01) in tumor samples, whereas 158 spots were down-regulated ( ratio <= -2, p <= 0.01). Seventy-one gene products were identified among these spots. Members of the heat shock protein 70 and 90 families were simultaneously up-regulated, whereas metabolism-associated proteins were decreased in HCC samples. The down-regulation of mitochondrial and peroxisomal proteins in these results suggested loss of special organelle functions during HCC carcinogenesis. Four metabolic enzymes involved in the methylation cycle in the liver were down-regulated in HCC tissues, indicating S-adenosylmethionine deficiency in HCC. Two gene products, glyceraldehyde-3-phosphate dehydrogenase and formimidoyltransferase-cyclodeaminase, were identified from inversely altered spots, suggesting that different isoforms or post-translational modifications of these two proteins might play different roles in HCC. For the first time, the overexpression of Hcp70/Hsp90-organizing protein and heterogeneous nuclear ribonucleoproteins C1/C2 in HCC tissues was confirmed by Western blot and then by immunohistochemistry staining in 70 HCC samples, suggesting their potential as protein tumor markers. In summary, we profiled proteome alterations in HCC tissues, and these results may provide useful insights for understanding the mechanism involved in the process of HCC carcinogenesis.

语种英语
WOS记录号WOS:000250092600013
引用统计
被引频次:159[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64949
专题北京大学基础医学院_细胞生物学系
作者单位1.Beijing Canc Hosp, Beijing 100036, Peoples R China
2.Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China
3.Beijing Inst Radiat Med, Beijing Proteome Res Ctr, State Key Lab Proteom, Beijing 102206, Peoples R China
4.Peking Univ, Dept Cell Biol, Hlth Sci Ctr, Beijing 100083, Peoples R China
5.Peking Univ, Dept Pathol, Hlth Sci Ctr, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Sun, Wei,Xing, Baocai,Sun, Yi,et al. Proteome analysis of hepatocellular carcinoma by two-dimensional difference gel electrophoresis[J]. MOLECULAR &amp; CELLULAR PROTEOMICS,2007,6(10):1798-1808.
APA Sun, Wei.,Xing, Baocai.,Sun, Yi.,Du, Xiaojuan.,Lu, Min.,...&He, Fuchu.(2007).Proteome analysis of hepatocellular carcinoma by two-dimensional difference gel electrophoresis.MOLECULAR & CELLULAR PROTEOMICS,6(10),1798-1808.
MLA Sun, Wei,et al."Proteome analysis of hepatocellular carcinoma by two-dimensional difference gel electrophoresis".MOLECULAR & CELLULAR PROTEOMICS 6.10(2007):1798-1808.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Sun, Wei]的文章
[Xing, Baocai]的文章
[Sun, Yi]的文章
百度学术
百度学术中相似的文章
[Sun, Wei]的文章
[Xing, Baocai]的文章
[Sun, Yi]的文章
必应学术
必应学术中相似的文章
[Sun, Wei]的文章
[Xing, Baocai]的文章
[Sun, Yi]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。