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学科主题: 基础医学
题名:
Proteome analysis of hepatocellular carcinoma by two-dimensional difference gel electrophoresis
作者: Sun, Wei; Xing, Baocai; Sun, Yi; Du, Xiaojuan; Lu, Min; Hao, Chunyi; Lu, Zhuang; Mi, Wei; Wu, Songfeng; Wei, Handong; Gao, Xue; Zhu, Yunping; Jiang, Ying; Qian, Xiaohong; He, Fuchu
刊名: MOLECULAR & CELLULAR PROTEOMICS
发表日期: 2007-10-01
DOI: 10.1074/mcp.M600449-MCP200
卷: 6, 期:10, 页:1798-1808
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEINS ; HEAT-SHOCK PROTEINS ; MASS-SPECTROMETRY ; GENE-EXPRESSION ; MESSENGER-RNA ; HEPATITIS-C ; CELL-LINE ; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE ; BIOENERGETIC SIGNATURE ; LIVER-CIRRHOSIS
英文摘要:

Hepatocellular carcinoma (HCC) is a highly malignant tumor, and chronic infection with hepatitis B virus is one of its major risk factors. To identify the proteins involved in HCC carcinogenesis, we used two-dimensional fluorescence DIGE to study the differentially expressed proteins in tumor and adjacent nontumor tissue samples. Samples from 12 hepatitis B virus-associated HCC patients were analyzed. A total of 61 spots were significantly up-regulated ( ratio >= 2, p <= 0.01) in tumor samples, whereas 158 spots were down-regulated ( ratio <= -2, p <= 0.01). Seventy-one gene products were identified among these spots. Members of the heat shock protein 70 and 90 families were simultaneously up-regulated, whereas metabolism-associated proteins were decreased in HCC samples. The down-regulation of mitochondrial and peroxisomal proteins in these results suggested loss of special organelle functions during HCC carcinogenesis. Four metabolic enzymes involved in the methylation cycle in the liver were down-regulated in HCC tissues, indicating S-adenosylmethionine deficiency in HCC. Two gene products, glyceraldehyde-3-phosphate dehydrogenase and formimidoyltransferase-cyclodeaminase, were identified from inversely altered spots, suggesting that different isoforms or post-translational modifications of these two proteins might play different roles in HCC. For the first time, the overexpression of Hcp70/Hsp90-organizing protein and heterogeneous nuclear ribonucleoproteins C1/C2 in HCC tissues was confirmed by Western blot and then by immunohistochemistry staining in 70 HCC samples, suggesting their potential as protein tumor markers. In summary, we profiled proteome alterations in HCC tissues, and these results may provide useful insights for understanding the mechanism involved in the process of HCC carcinogenesis.

语种: 英语
WOS记录号: WOS:000250092600013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64949
Appears in Collections:基础医学院_细胞生物学系_期刊论文

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作者单位: 1.Beijing Canc Hosp, Beijing 100036, Peoples R China
2.Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China
3.Beijing Inst Radiat Med, Beijing Proteome Res Ctr, State Key Lab Proteom, Beijing 102206, Peoples R China
4.Peking Univ, Dept Cell Biol, Hlth Sci Ctr, Beijing 100083, Peoples R China
5.Peking Univ, Dept Pathol, Hlth Sci Ctr, Beijing 100083, Peoples R China

Recommended Citation:
Sun, Wei,Xing, Baocai,Sun, Yi,et al. Proteome analysis of hepatocellular carcinoma by two-dimensional difference gel electrophoresis[J]. MOLECULAR &amp; CELLULAR PROTEOMICS,2007,6(10):1798-1808.
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