北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学临床肿瘤学院  > 期刊论文
学科主题: 临床医学
题名:
Antitumour effects of Yangzheng Xiaoji in human osteosarcoma: The pivotal role of focal adhesion kinase signalling
作者: Jiang, Wen G.1,2; Ye, Lin1,2; Ji, Ke1,2; Ruge, Fiona1,2; Wu, Yiling3; Gao, Yong3; Ji, Jiafu1,4; Mason, Malcolm D.1,2
关键词: osteosarcoma ; in vivo model ; Yangzheng Xiaoji ; cell adhesion ; focal adhesion kinase ; electric cell-substrate impedance sensing ; paxillin ; integrin
刊名: ONCOLOGY REPORTS
发表日期: 2013-09-01
DOI: 10.3892/or.2013.2586
卷: 30, 期:3, 页:1405-1413
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: CANCER-CELLS ; IN-VITRO ; GROWTH ; ANGIOGENESIS ; INHIBITION ; IMPACT ; METASTASIS ; EXPRESSION ; MIGRATION ; ADJUVANT
英文摘要:

The present study examined, in vitro and in vivo, the potential antitumour effects of Yangzheng Xiaoji (YZXJ), a traditional Chinese medical formula used in cancer treatment, on osteosarcoma, a tumour type recently found to be sensitive to YZXJ. The human osteosarcoma cell line MG63 was used in cell-matrix adhesion and cell growth assays. The same cell line was used in an in vivo tumour model by establishing subcutaneous osteosarcoma xenografts. Oral and intraperitoneal routes were used to deliver the YZXJ extract. The effect of YZXJ on the activation of focal adhesion kinase (FAK) and paxillin was evaluated by immunofluorescence methods. It was found that YZXJ exhibited a significant inhibitory effect on cell-matrix adhesion as demonstrated by a cell-based assay and electric cell-substrate impedance sensing (ECIS) analysis. The effect was observed together with a reduction in phospho-FAK and phospho-paxillin in the cells when treated with YZXJ. In the in vivo tumour model, YZXJ was found to significantly inhibit the growth of osteosarcoma with a sustained effect observed when YZXJ was delivered intraperitoneally. YZXJ sensitized cells to the effect of FAK inhibitor in vitro and in vivo. It is concluded that Yangzheng Xiaoji plays a significant role in cell-matrix adhesion and tumour growth, likely by inhibiting the activation of the FAK pathway. The therapeutic role of Yangzheng Xiaoji in osteosarcoma warrants further investigation.

语种: 英语
项目资助者: Cancer Research Wales ; Albert Hung Foundation
WOS记录号: WOS:000322384700050
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64952
Appears in Collections:北京大学临床肿瘤学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Cardiff Univ Peking Univ Sch Oncol Joint Inst, Beijing, Peoples R China
2.Cardiff Univ, Sch Med, Inst Canc & Genet, Metastasis & Angiogenesis Res Grp, Cardiff CF14 4XN, S Glam, Wales
3.Yiling Med Res Inst Tianshan Dajie, Shijiazhuang, Hebei, Peoples R China
4.Peking Univ, Canc Hosp, Dept Gastroenterol Oncol, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100871, Peoples R China

Recommended Citation:
Jiang, Wen G.,Ye, Lin,Ji, Ke,et al. Antitumour effects of Yangzheng Xiaoji in human osteosarcoma: The pivotal role of focal adhesion kinase signalling[J]. ONCOLOGY REPORTS,2013,30(3):1405-1413.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Jiang, Wen G.]'s Articles
[Ye, Lin]'s Articles
[Ji, Ke]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Jiang, Wen G.]‘s Articles
[Ye, Lin]‘s Articles
[Ji, Ke]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace