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学科主题: 临床医学
题名:
Urotensin II increases endothelin production by vascular smooth muscle cells in rats
作者: Qi, YF; Bu, DF; Yang, J; Zhang, ZK; Niu, D; Shi, YR; Zhang, ZH; Tang, CS; Du, JB
关键词: urotensin II ; endothelin ; mRNA ; peptide/interaction
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2002-06-01
卷: 47, 期:12, 页:1007-1010
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: ENDOGENOUS LIGAND ; ORPHAN RECEPTOR ; COMPETITIVE PCR ; GPR14
英文摘要:

The aim of this study was to observe the effects of urotensin 11 (01) on production of endothelin (ET) in rat aortic vascular smooth muscle cells (VSMC). Cultured VSMCs incubated with various concentrations of UII were used to measure the VSMC H-3-TdR incorporation, the amount of ET mRNA and ET production in VSMCs. In this work we found that UII (10-(10)-10(-8) moL/L) promoted VSMC H-3-TdR incorporation (47%-83%, P < 0.01) and increased the amount of ET mRNA by 17.1% (P < 0.05) to 112.8% (P < 0.01), respectively, in a concentration dependent manner compared with control. After 4 and 8 h incubation, 10(-10)-10(-8) mol/L of UII elevated the ET synthesis and release in a concentration dependent manner. After 4 h incubation, the content of ET in medium was 4.9, 5.36 and 7.12 pg/mL (P < 0.01). After 8 h incubation, the ET content released from VSMCs was 12.6, 12.07 and 17.17 pg/mL (P < 0.01). In addition, it was found that BQ(123), a specific ETA receptor antagonist, obviously decreased the VSMC DNA synthesis Induced by UII. The results of this study showed that UII could stimulate the ET mRNA expression and ET production in VSMC. The effects of UII on VSMC DNA synthesis were partly mediated by ET autocrine pathway. It suggests that the interaction between UII and ET plays an important biological regulating role as endogenous active peptides.

语种: 英语
WOS记录号: WOS:000175994600010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64969
Appears in Collections:北京大学第一临床医学院_心血管内科_期刊论文

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作者单位: 1.Phoenix Pharmaceut Inc, Belmont, CA 94002 USA
2.Peking Univ, Hosp 1, Inst Cardiovasc Dis, Beijing 100083, Peoples R China
3.Peking Univ, Ctr Hlth Sci, Dept Physiol, Beijing 100034, Peoples R China

Recommended Citation:
Qi, YF,Bu, DF,Yang, J,et al. Urotensin II increases endothelin production by vascular smooth muscle cells in rats[J]. CHINESE SCIENCE BULLETIN,2002,47(12):1007-1010.
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