北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 北京大学人类疾病基因研究中心  > 期刊论文
学科主题: 基础医学
题名:
PTPIP51, a novel 14-3-3 binding protein, regulates cell morphology and motility via Raf-ERK pathway
作者: Yu, Chuanfei1,2; Han, Wenling1,2; Shi, Taiping1,2,3; Lv, Bingfeng1,2; He, Qihua4; Zhang, Yanfei1,2; Li, Ting1,2; Zhang, Yingmei1,2; Song, Quansheng1,2; Wang, Lu1,2; Ma, Dalong1,2,3
关键词: 14-3-3 ; Raf-1 ; ERK ; Migration
刊名: CELLULAR SIGNALLING
发表日期: 2008-12-01
DOI: 10.1016/j.cellsig.2008.07.020
卷: 20, 期:12, 页:2208-2220
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: TYROSINE-PHOSPHATASE 1B ; EPIDERMAL-GROWTH-FACTOR ; NEGATIVE REGULATION ; IN-VIVO ; PHOSPHORYLATION SITES ; BREAST-CANCER ; PC12 CELLS ; KINASE-C ; ACTIVATION ; MIGRATION
英文摘要:

Cell migration plays a critical role during the development of most organisms and the process of malignant tumor metastasis. In the present study, we investigated the role of PTPIP51 (protein tyrosine phosphatase interacting protein 51) in cell motility. Overexpression of PTPIP51 induced cell elongation, increased cell migration, adhesion, and spreading, while downregulation of PTPIP51 had the opposite effects. We demonstrated here, that PTPIP51 could regulate ERK activity on Raf level, since MEK inhibitor and dominant-negative Raf-1 but not Ras could inhibit the ERK activation induced by PTPIP51. Further studies proved that PTPIP51 could interact with Raf-1 through 14-3-3, suggesting that PTPIP51 is a regulator of the Raf-MEK-ERK cascade through modulation of Raf-1 by 14-3-3. In addition, two redundant 14-3-3 binding domains in the PTPIP51 protein have been identified by deletion/mutation studies. We conclude that PTPIP51 regulates cell morphology and cell motility via interaction with Raf-1 through 14-3-3, and that PTPIP51 binds to 14-3-3 through two redundant binding domains. (c) 2008 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 2006AA02A305
项目资助者: National High Technology Research and Development Program of China (863 Program)
WOS记录号: WOS:000261560800005
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/64974
Appears in Collections:基础医学院_北京大学人类疾病基因研究中心_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
PTPIP51, a novel 14-3-3 binding protein, regulates cell morphology and motility via Raf-ERK pathway.pdf(2429KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Chinese Natl Human Genome Ctr, Beijing 100176, Peoples R China
2.Peking Univ, Ctr Human Dis Genom, Beijing 100191, Peoples R China
3.Peking Univ, Lab Med Immunol, Sch Basic Med Sci, Hlth Sci Ctr, Beijing 100191, Peoples R China
4.Peking Univ, Hlth & Med Anal Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Yu, Chuanfei,Han, Wenling,Shi, Taiping,et al. PTPIP51, a novel 14-3-3 binding protein, regulates cell morphology and motility via Raf-ERK pathway[J]. CELLULAR SIGNALLING,2008,20(12):2208-2220.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Yu, Chuanfei]'s Articles
[Han, Wenling]'s Articles
[Shi, Taiping]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Yu, Chuanfei]‘s Articles
[Han, Wenling]‘s Articles
[Shi, Taiping]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace