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学科主题基础医学
Changes of ATP and ADP in cultured astrocytes under and after in vitro ischemia
Yu, ACH; Lau, AMF; Fu, AWY; Lau, LT; Lam, PY; Chen, XQ; Xu, ZY
关键词astrocytes culture in vitro ischemia energy metabolism ATP
刊名NEUROCHEMICAL RESEARCH
2002-12-01
27期:12页:1663-1668
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Neurosciences
研究领域[WOS]Biochemistry & Molecular Biology ; Neurosciences & Neurology
关键词[WOS]NECROSIS-FACTOR-ALPHA ; CELL-CULTURES ; INJURY ; NEURONS ; INTERLEUKIN-6 ; GLUTAMATE ; IMMATURE ; HYPOXIA
英文摘要

A very large body of evidence from in vivo studies has been accumulated on a link between the change of energy and cell survival/apoptosis. Using an in vitro ischemia model, we have previously shown that ischemia could induce apoptosis in astrocytes. In this study, we utilized the same in vitro model to investigate changes in ATP and ADP levels in cultured astrocytes and attempted to demonstrate an energy-cell death linkage. Astrocytes remained unaltered after 2 hr of ischemia but were moderately or severely damaged after 4 or 6-8 hr, respectively. The astrocytes that survived various lengths of in vitro ischemic incubation retained their ability to produce ATP after ischemia. Both ATP and ADP levels were increased in astrocytes that remained alive under in vitro ischemia for over 6 hr. The largest decline in the percent of viable astrocytes during ischemia corresponded well to the reduction in ATP and ADP levels in these cultures.

语种英语
WOS记录号WOS:000179862800015
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被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/64992
专题北京大学基础医学院_神经生物学系
作者单位1.Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
2.Peking Univ, Dept Neurobiol, Beijing 100083, Peoples R China
3.Hong Kong DNA Chips Ltd, Hong Kong, Hong Kong, Peoples R China
4.Hong Kong Univ Sci & Technol, Dept Biol, Kowloon, Hong Kong, Peoples R China
推荐引用方式
GB/T 7714
Yu, ACH,Lau, AMF,Fu, AWY,et al. Changes of ATP and ADP in cultured astrocytes under and after in vitro ischemia[J]. NEUROCHEMICAL RESEARCH,2002,27(12):1663-1668.
APA Yu, ACH.,Lau, AMF.,Fu, AWY.,Lau, LT.,Lam, PY.,...&Xu, ZY.(2002).Changes of ATP and ADP in cultured astrocytes under and after in vitro ischemia.NEUROCHEMICAL RESEARCH,27(12),1663-1668.
MLA Yu, ACH,et al."Changes of ATP and ADP in cultured astrocytes under and after in vitro ischemia".NEUROCHEMICAL RESEARCH 27.12(2002):1663-1668.
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